Correlation of Lipoprotein Abnormalities in Childhood withe the Risk for Atherosclerotic Coronary Heart Disease (CAD) in Adulthood (Is abnormaltites in childhood a risk factor for CAD? )

1.我们研究了脂蛋白A-Ⅰ（仅含载脂蛋白A-Ⅰ的高密度脂蛋白（HDL））亚类对血浆胆固醇酯化和泡沫细胞胆固醇净流出的影响。LpA-I由三种不同尺寸的颗粒组成：大（Lg-LpA-I）、中（Md-LpA-I）和小（Sm-LpA-I）。血浆LpA-I浓度仅与Lg-LpA-I水平呈正相关。血浆Lg-LpA-I浓度与血浆及VLDL和LDL耗竭血浆中胆固醇酯化率（FERplasma和FER_1）呈负相关<HDL>。当巨噬泡沫细胞与Md-和Sm-LpA-I孵育时，细胞胆固醇质量减少约70%。相反，Lg-LpA-I的细胞胆固醇降低能力可以忽略不计。Lg-LpA-I抑制泡沫细胞的净胆固醇清除，这是由Md-和Sm-LpA-I介导的，并与这些颗粒的胆固醇酯的产生。这些结果表明，Md-和Sm-LpA-I可能积极参与了 关于我们 Lg-LpA-I可能调节Md-1和Sm-LpA-I的上述功能。2.我们研究了低密度脂蛋白（LDL）颗粒大小与FER_2<HDL>和血脂水平的关系。血浆<HDL>胆固醇与LDL颗粒大小呈负相关（r=-0.728、-0.598和0.572，P&gt;0.0001）。多元逐步回归分析显示，FER_<HDL>与LDL颗粒大小的相关性最显著。由于FER_1<HDL>受血浆LpA-I浓度的调节，（HDL含有apoA-I但不含apoA-II），其是HDL的抗动脉粥样硬化组分，目前的结果表明，LDL颗粒大小的调节可能是LpA-1.3抗动脉粥样硬化性质的一部分。低血浆LpA-I儿童的LpA-I定性和定量变化与冠状动脉疾病（CAD）患者的LpA-I定性和定量变化难以区分低血浆LpA-I的儿童是冠心病的高危人群。少

1.We investigated the effects of subclasses of LpA-I [high density lipoprotein (HDL) containing only apoA-I] on cholesterol esterification in plasma and net cholestrol efflux from foam cells. LpA-I was composed of particles of three different sizes : large (Lg-LpA-I), medium (Md-LpA-I) and small (Sm-LpA-I). Plasma concentrations of LpA-I were positively correlated only with the level of Lg-LpA-I.Plasma concentrations of Lg-LpA-I were inversely correlated with the rate of cholesterol esterification in plasma and VLDL and LDL-depleted plasma (FERplasma and FER_<HDL>). When macrophage foam cells were incubated with Md-and Sm-LpA-I,cellular cholesterol mass was reduced by approximately 70%. In contrast, the cellular cholesterol-reducing capacity of Lg-LpA-I was negligible. Lg-LpA-I inhibited net cholesterol removal from foam cells that was mediated by Md-and Sm-LpA-I and cholesteryl ester production with these particles. These results suggest that Md-and Sm-LpA-I may actively participate i … More n cellular cholesterol removal and cholesterol esterification in plasma and HDL,while Lg-LpA-I may regulate these functions of Md-and Sm-LpA-I.2.We examined the correlations between low-density-lipoprotein (LDL) particle size and both FER_<HDL> and plasma lipid levels. FER_<HDL> and plasma triglyceride were negatively, and HDL-cholesterol was positively, correlated with LDL particle size (r=-0.728, -0.598and0.572, p>0.0001). Stepwise multiple regression analysis revealed that FER_<HDL> was most significantly associated with LDL particle size. Since FER_<HDL> is regulated by the plasma consentration of LpA-I (HDL containig apoA-I but not apoA-II), which is the anti-atherogenic fraction of HDL,the present results suggest that the regulation of LDL particle size may be part of the anti-atherogenic nature of LpA-1.3.Qualitative and quantitative changes of LpA-I in children with low plasma LpA-I were indistinguishable from those in patients with coronary artery disease (CAD) with low plasma LpA-I.All of our data suggest that children with low plasma LpA-I are high risk for CAD. Less