Research on Therapeutic Mechanisms of Ultraviolet Therapy

1.补骨脂素光化学疗法联合维生素D3软膏治疗寻常型银屑病的临床研究一项左右对照研究表明，补骨脂素光化学疗法联合他卡西醇软膏治疗寻常型银屑病的疗效优于单纯使用PUVA+类固醇软膏或赋形剂的疗效。组织学和免疫组织化学研究表明，PUVA+TL治疗后皮损中表皮增厚、Ki-67+细胞、浸润性白细胞和细胞黏附分子表达增加的数量或程度明显低于PUVA+ST或V治疗后。PUVA+TL的治疗机制可能与其抗增殖和免疫调节作用有关。环孢素A联合紫外光照射环孢素A(CyA，5~50 mg/kg/d)或单用赋形剂(V)对BALB/c小鼠连续5天的治疗作用，小鼠耳暴露于中波紫外线(100~500mJ/sq cm)。经UVB照射后，5-溴脱氧尿苷(BrdU)+细胞数在短暂抑制后24小时显著增加。CyA可显著抑制UVB诱导的BrdU+细胞增殖，而V不是单独作用。CyA+UVB较单独处理显著降低朗格汉斯细胞(LC)数。CyA和UVB可能协同抑制表皮增殖和LC计数的增加，这两者都是银屑病的部分原因。

1. Clinical Research on Therapeutic Mechanisms of Psoraren Photochemotherapy (PUVA) Combined With Vitamin D3 Ointment in Psoriasis VulgarisA right-left comparative study in psoriasis vulgaris demonstrated that PUVA + tacalcitol ointment (TL) was better than PUVA + steroid ointment (ST) or vehicle (V) alone. Histological and immunohistochemical studies showed that the increased number or degree of epidermal thickness, Ki-67+ cells, infiltrating leukocytes, and cell adhesion molecule expression in pretreated lesion was reduced to a greater extent after PUVA + TL than after PUVA + ST or V.Therapeutic mechanisms of PUVA + TL may be attributed to both antiproliferative and immunomodulatory effects.2. Basic Research on Therapeutic Mechanisms of Cyclosporin A Combined With Ultraviolet-B-PhotocherapyCyclosporin A (CyA,5-50 mg/kg/day) or vehicle (V) alone was administered per os to BALB/c mice for 5 days, and mouse ears were exposed to UVB (100-500 mJ/sq cm). UVB radiation inceased the number of 5-bromodeoxyuridine (BrdU)+ cells at 24h after transient suppression. CyA,but not V alone, significantly suppressed UVB-induced increment in BrdU+ cells. Cya+UVB significantly decreased Langerhans cell (LC) coants than each treatment alone. CyA and UVB may cooperate to suppress increased epidermal proliferation and LC counts, both of which are partly responsible for psoriasis.