Development of loricrin monoclonal antibody and its specific location

兜甲素单克隆抗体的研制及其具体定位

• 批准号: 06670850
• 负责人: SAKAI Hiroyuki
• 金额: $ 0.83万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670850/
• 关键词: psoriasis; loricrin; involucrin; cornified cell enrelope; protein kinase C; 単クローン抗体; ハイブリドーマ

We analyzed CE formation in normal skin and in lesional psoriatic skin by immunoelectron microscopy. In the superficial granular cells of normal epidermis, involucrin labeling was detected along the plasma membrane, whereas loricrin staining was mostly distributed diffusely within the cells, occasionally with some additional granular aggregates within the cytoplasm and nucleus. Loricrin was also present predominantly on the desmosomal attachment plaques, colocalizing with desmoglein. In the cornified cells, involucrin labeling was reduced, whereas loricrin labeling was retained and continuously decorated the CEs, with relative sparing of the desmosomal areas. In typical psoriatic epidermis, involucrin staining was very intense but loricrin labeling was markedly reduced. The involucrin-positive CEs were formed precociously and further maturation of CE did not occur.

我们用免疫电子显微镜分析了正常皮肤和银屑病皮损中CE的形成。在正常表皮的浅层颗粒细胞中，总蛋白标记沿细胞膜分布，而氯蛋白染色主要弥漫分布于细胞内，偶见胞浆和胞核内有少量颗粒聚集。Loricrin也主要出现在桥粒附着斑块上，与桥粒芯糖蛋白共存。在角化细胞中，总蛋白的标记减少，而氯蛋白的标记被保留，并继续装饰细胞，相对较少的桥粒区域。在典型的银屑病皮损中，包皮蛋白染色很深，而氯丙蛋白的标记明显减少。包膜蛋白阳性的CEs早熟形成，未发生进一步成熟。