Antisense Therapy for Reperfusion Injury following Liver Transplantation

为了选择合适的反义探针，检测了17mer-1-PO、PS、PS3和17mer-2-PO、18mer-PS3、30mer-PS3的TNFα、P50-PS3、P65-PS3的反义寡核苷酸以及针对NF-kB的三链形成的寡核苷酸对TNFα产生的反义作用。这些反义基因都没有显示出有效的抑制作用，脂质体对寡核苷酸的摄取还不够。我们未能获得有效的反义脂质体用于体内研究。在本研究中，我们通过原位杂交成功地检测到了肿瘤坏死因子α基因的信号，该方法将在不久的将来应用于临床。

To select appropriate antisense probe, the following oligonucleotides were examined ; 17mer-1-PO,PS,PS3 and 17mer-2-PO,18mer-PS3,30mer-PS3 for TNFalpha, P50-PS3, P65-PS3 and triplex forming oligonucleotides for NF-kB.Antisense effect on TNFalpha Production was examined using RAW264 cell-lines with LPS stimulation. None of these antisenses showed effective suppression.Oligonucleotide uptake by liposomes was not so enough. We failed to get effective antisense liposome for in vivo study. In this study, we succeeded to detect signals from TNFalpha mRNA by in situ hybridization.This method will be used in clinic in the near future.