Antisense Therapy for Reperfusion Injury following Liver Transplantation

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To select appropriate antisense probe, the following oligonucleotides were examined ; 17mer-1-PO,PS,PS3 and 17mer-2-PO,18mer-PS3,30mer-PS3 for TNFalpha, P50-PS3, P65-PS3 and triplex forming oligonucleotides for NF-kB.Antisense effect on TNFalpha Production was examined using RAW264 cell-lines with LPS stimulation. None of these antisenses showed effective suppression.Oligonucleotide uptake by liposomes was not so enough. We failed to get effective antisense liposome for in vivo study. In this study, we succeeded to detect signals from TNFalpha mRNA by in situ hybridization.This method will be used in clinic in the near future.