Evaluation of anticancer agents against the colon cancer using the analysis of DNA ploidy and apoptosis.

使用 DNA 倍性和细胞凋亡分析来评估抗结肠癌的抗癌药物。

• 批准号: 06671276
• 负责人: MAKINO Masato
• 金额: $ 0.45万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671276/
• 关键词: Apoptosis; Colorectal cancer; 5-fluorouracil; Chemotherapy; 大腸癌化学療法; 細胞核DNA

We examined whether the administration of 5-fluorouracil (5-FU) could enhance apoptosis of colorectal cancer cells, and we investigated the relationship between apoptosis and the expression of Ki-67, p53, and c-myc. Twenty patients with advanced colorectal cancer were divided into two groups. Ten received continuous intravenous 5-FU at 500 mg/body/day for 7 days preoperatively and 10 did not receive 5-FU and served as controls. Apoptotic cells were stained by so-called TUNEL method (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling). Apoptosis of cancer cells increased signinficantly to 18 <plus-minus> 6% in the 5-FU-treated group as compare to the controls (3 <plus-minus> 1% ; p<0.001). Expression of Ki-67, a indicator of cell proliferation, was inversely correlated with the TUNEL staining that was specific for apoptosis and the percentage of cells immunostained for Ki-67 fell significantly to 34 <plus-minus> 14% of the apoptotic area as compared to 72 <plus-minus> 12% in controls (p<0.001). There was no clear correlation between expression of apoptosis and the expression of p53 o

我们研究了5-氟尿嘧啶（5-FU）的管理是否可以增强大肠癌细胞的凋亡，我们研究了凋亡和Ki-67，p53，c-myc的表达之间的关系。将20例晚期结直肠癌患者分为两组。10例患者术前连续静脉注射5-FU 500 mg/人/天，持续7天，10例未接受5-FU，作为对照。凋亡细胞通过所谓的TUNEL法（末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记）染色。5-FU组肿瘤细胞凋亡率为<plus-minus>18.6%，明显高于对照组的3.1%<plus-minus>（P&lt;0.001）。Ki-67的表达是细胞增殖的一个指标，与凋亡特异性的TUNEL染色呈负相关，Ki-67免疫染色的细胞百分比显著下降至<plus-minus>凋亡面积的34 - 14%，而对照组为72 <plus-minus>- 12%（p&lt;0.001）。凋亡与p53 o表达无明显相关性

项目成果

M. Makino, et al.: "Increased induction of apoptosis of human colorectal cancer cells after preoperative treatment with 5-fluorouracil." ONCOLOGYREPORTS. 3. 281-285 (1996)

M. Makino 等人：“术前使用 5-氟尿嘧啶治疗后，可增加对人结直肠癌细胞凋亡的诱导。”

M.Makino, et al.: "Increased induction of apoptosis of human colorectal cancer cells after preoperative treatment with 5-fluorouracil." ONCOLOGY REPORTS. 3. 281-285 (1996)

M.Makino 等人：“术前使用 5-氟尿嘧啶治疗后，可增加对人结直肠癌细胞凋亡的诱导。”

M.Makino,et al.: "Increased induction of apoptosis of human colorectal cancer cells after preoperative treatment with 5-fluorouracil." ONCOLOGY REPORTS. 3. 281-285 (1996)

M.Makino 等人：“术前使用 5-氟尿嘧啶治疗后，可增加对人结直肠癌细胞凋亡的诱导。”