ESTABLISHMENT AND CHARACTERIZATION OF CULTURED VASCULAR ENDOTHELIAL CELLS DERIVED FROM PATIENTS WITH MOYAMOYA DISEASE

(1)我们培养并建立了三个来自烟雾病患者的人血管内皮细胞。从搭桥手术中获得的网膜毛细血管中培养内皮细胞。研究了细胞的一般生物学特性，包括增殖潜力，并与对照患者的细胞进行了比较。我们目前未发现烟雾病患者和对照组细胞的生物学特性有任何差异。我们现在正在研究其他特性，包括内皮细胞的渗透性和粘附分子的表达。这一结果将有助于阐明烟雾病早期内膜增厚的机制。(2)我们对烟雾病患者的颞浅动脉进行了组织学检查和电镜观察。烟雾病患者内膜增厚的年龄明显早于对照组。烟雾病患者血管平滑肌细胞（SMC）从中膜向内膜迁移和增殖。在烟雾病增厚的血管内膜中观察到平滑肌细胞的合成表型。结果表明，系统因素，促进迁移和增殖的平滑肌细胞在烟雾病。(3)我们研究了兔颈动脉球囊内皮剥脱后内膜增厚的发展。结果发现，内膜平滑肌细胞增殖期结束后进入静止期，其弹性蛋白的形成和弹性蛋白原基因的表达增加。这一结果有助于了解烟雾病患者增厚内膜中多层弹性膜的形成机制。

(1) We cultured and established three human vascular endothelial cells derived from patients with moyamoya disease. The endothelial cells were cultured from the capillary of the omentum obtained during bypass surgeries. General biological characteristics of the cells, including proliferative potentials, were investigated and compared with cells from control patients. We have found no difference in the biological charcteristics between cells of moyamoya and control patients presently. We are now investigating other charcteristics, including the permeability and the expression of adhesion molecules of the endothelial cells. The results will be potentially useful in elucidating the mechanism of early development of intimal thickening in moyamoya disease. (2) We histologically examined superficial temporal arteries of moyamoya patients with electron microscopies and immunohistochemistries. Intimal thickenings appeared significantly earlier of age in moyamoya patients than in controls. Migration and proliferation of smooth muscle cells (SMCs) from the media to the intima was often seen in moyamoya patients. Synthetic phenotype of SMCs was obesrved in the thickened intima of moyamoya disease. The results indicate systemic factors that promote migration and proliferation of SMCs in moyamoya disease. (3) We examined the development of intimal thickening after balloon endothelial denudation in rabbit carotid arteries. We found that elastin formation and the expression of tropoelastin gene increased in SMCs entering quiescence after the end of the proliferative phase in the intima. The results will be of help in understanding the mechanisms of formation of multilayred elastic lamina in the thickend intima of moyamoya patients.