QUANTITATIVE EVALUATION FOR INFLAMMATORY ACTIVITIES USING A CHEMILUMINESCENT METHOD IN RAT'S AIR POUCH MODEL.

1994年，探测者布置了一个光多增强器、一个光计数器、一个光导和一个暗盒，组成了一个化学发光测量系统。在暗箱中的大鼠气囊模型中，用MCLA作为化学发光的增加因子，该系统具有测量化学发光的能力。MCLA是一种非常灵敏的材料，其浓度对检测超氧化物的奇异性有很大影响。1995年研究的目的是建立适当的测量方法。侵袭者在6~8周龄大鼠背部制作气囊模型，体重200~240g，用苦味素A和微小棒状杆菌刺激气囊中的白细胞。7日龄气囊内注入2mlPicnin-A和短小棒状杆菌溶液。浓度分别为0.1%、0.5%、1.0%。在注射Picnin-A溶液后3、6、12、24和48小时，以及注射微小棒状杆菌溶液48小时后，用2毫升20mU的MCLA测定化学发光，化学发光强度随Picnin-A和棒状杆菌浓度的增加而增强。刺激剂的种类影响化学发光量。注射刺激剂和测量之间的时间不影响化学发光量。

The invastigators arranged a photo-multi-intensifier, a photo-counter, a light guide and a dark-box to make up a chemiluminescent measuring system in 1994. The system was revealed to have a capability of measuring the chemiluminescence using MCLA as a increasing factor for the chemiluminescence in the rat's air pouch model in the dark-box. The MCLA is very sensitive material, and its concentration effects much to the singularity of detecting the superoxide. The pourpose of the study during 1995, is to establish the adequate methods of the measurement. The invastigators made a air pouch model on the back of the rat at age of 6 to 8 weeks, the body weight of which were 200 to 240 g. Picnin-A and Corynebacterium parvum were used as a stimulant for the leukocytes in the air pourch. Two ml of Picnin-A and Corynebacterium parvum solution were injected into 7-days-old air pouch. The concentrations of the Picnin-A and Corynebacterium parvum solution were 0.1%, 0.5% and 1.0%. At 3,6,12,24 and 48 hours after injecting a Picnin-A solution, and at 48 hours after injecting a Corynebacterium parvum solution, the chemiluminescences were measured using 2 ml of 20mus of MCLA.The chemiluminescence incresed as the consentration of Picnin-A and Corynebacterium parvum increased. The kind of stimulant influenced the amount of chemilumineses. The duration between the injection of the stimulant and measurement did not effect the amount of chemiluminescence.