QUANTITATIVE EVALUATION FOR INFLAMMATORY ACTIVITIES USING A CHEMILUMINESCENT METHOD IN RAT'S AIR POUCH MODEL.

1994年，研究人员将一个光多增强器、一个光计数器、一个导光板和一个暗箱组成了一个化学发光测量系统。实验结果表明，该系统能够以MCLA为增加因子测量暗箱中大鼠气囊模型的化学发光。MCLA是一种非常敏感的材料，其浓度对超氧化物检测的奇异性有很大影响。1995年研究的目的是建立适当的测量方法。6 ~ 8周龄大鼠，体重200 ~ 240 g，在大鼠背部制作气囊模型。用皮宁- a和小棒状杆菌作为刺激物刺激气袋中的白细胞。将匹宁- a和细小棒状杆菌溶液2 ml注射到7 d龄的气囊中。松果素- a和细小棒状杆菌溶液的浓度分别为0.1%、0.5%和1.0%。在注射匹克林- a溶液后3、6、12、24和48小时，以及注射细小棒状杆菌溶液后48小时，使用2ml 20mus MCLA测量化学发光。化学发光随匹克林- a和细小棒状杆菌浓度的增加而增加。刺激物的种类影响化学发光的数量。注射兴奋剂和测量之间的时间不影响化学发光的量。

The invastigators arranged a photo-multi-intensifier, a photo-counter, a light guide and a dark-box to make up a chemiluminescent measuring system in 1994. The system was revealed to have a capability of measuring the chemiluminescence using MCLA as a increasing factor for the chemiluminescence in the rat's air pouch model in the dark-box. The MCLA is very sensitive material, and its concentration effects much to the singularity of detecting the superoxide. The pourpose of the study during 1995, is to establish the adequate methods of the measurement. The invastigators made a air pouch model on the back of the rat at age of 6 to 8 weeks, the body weight of which were 200 to 240 g. Picnin-A and Corynebacterium parvum were used as a stimulant for the leukocytes in the air pourch. Two ml of Picnin-A and Corynebacterium parvum solution were injected into 7-days-old air pouch. The concentrations of the Picnin-A and Corynebacterium parvum solution were 0.1%, 0.5% and 1.0%. At 3,6,12,24 and 48 hours after injecting a Picnin-A solution, and at 48 hours after injecting a Corynebacterium parvum solution, the chemiluminescences were measured using 2 ml of 20mus of MCLA.The chemiluminescence incresed as the consentration of Picnin-A and Corynebacterium parvum increased. The kind of stimulant influenced the amount of chemilumineses. The duration between the injection of the stimulant and measurement did not effect the amount of chemiluminescence.