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QUANTITATIVE EVALUATION FOR INFLAMMATORY ACTIVITIES USING A CHEMILUMINESCENT METHOD IN RAT'S AIR POUCH MODEL.

使用化学发光方法对大鼠气囊模型中的炎症活动进行定量评估。

基本信息

批准号：
06671438
负责人：
SHIRAKURA Kenji
金额：
$ 1.09万
依托单位：
GUNMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671438/
关键词：
ARTHRITIS SUPEROXIDE CHEMILUMIESCENCE POLYNUCLEAR LEUKOCYTE MCLA RAT AIR POUCH MODEL DIAGNOSIS ENDOSCOPY 活性酵素

项目摘要

The invastigators arranged a photo-multi-intensifier, a photo-counter, a light guide and a dark-box to make up a chemiluminescent measuring system in 1994. The system was revealed to have a capability of measuring the chemiluminescence using MCLA as a increasing factor for the chemiluminescence in the rat's air pouch model in the dark-box. The MCLA is very sensitive material, and its concentration effects much to the singularity of detecting the superoxide. The pourpose of the study during 1995, is to establish the adequate methods of the measurement. The invastigators made a air pouch model on the back of the rat at age of 6 to 8 weeks, the body weight of which were 200 to 240 g. Picnin-A and Corynebacterium parvum were used as a stimulant for the leukocytes in the air pourch. Two ml of Picnin-A and Corynebacterium parvum solution were injected into 7-days-old air pouch. The concentrations of the Picnin-A and Corynebacterium parvum solution were 0.1%, 0.5% and 1.0%. At 3,6,12,24 and 48 hours after injecting a Picnin-A solution, and at 48 hours after injecting a Corynebacterium parvum solution, the chemiluminescences were measured using 2 ml of 20mus of MCLA.The chemiluminescence incresed as the consentration of Picnin-A and Corynebacterium parvum increased. The kind of stimulant influenced the amount of chemilumineses. The duration between the injection of the stimulant and measurement did not effect the amount of chemiluminescence.

1994年，该实验室利用光电倍增管、光电计数器、光导管和暗箱组成了一套光致发光测量系统。在大鼠气袋模型中，采用MCLA作为化学发光的增强因子，在暗箱中显示该系统具有测量化学发光的能力。MCLA是一种非常敏感的物质，其浓度对超氧阴离子检测的灵敏度影响很大。1995年研究的目的是建立适当的测量方法。实验动物于6 ~ 8周龄体重200 ~ 240 g的大鼠背部制作气囊模型。用Picnin-A和短小棒状杆菌作为刺激剂，刺激空气中的白细胞。将2 ml的Picnin-A和短棒状杆菌溶液注射到7日龄的气囊中。Picnin-A和短棒状杆菌溶液的浓度分别为0.1%、0.5%和1.0%。在注射Picnin-A溶液后3、6、12、24、48小时和注射棒状杆菌溶液后48小时，用2 ml的20 μ mMCLA测定化学发光，随着Picnin-A和棒状杆菌浓度的增加，化学发光强度增加。刺激物的种类影响着神经元的分泌量。注射刺激物和测量之间的持续时间不影响化学发光的量。