Effects caused by mechanical stretch to the connective tissue

机械拉伸对结缔组织造成的影响

• 批准号: 11671417
• 负责人: SHIRAKURA Kenji
• 金额: $ 2.24万
• 依托单位: Gunma University Faculty of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671417/
• 关键词: Calcium ion; stretch; rat; fluo-3-acetoxymethyl; 結合組織

The importance of the mechanical environment to homeostasis of connective tissue is well known. While alterations in this environment are known to affect the biochemical and biomaterial characteristics of these tissue, the exact mechanism by which mechanical stress is mediated by the cell is still unclear. The current study investigates the effect of in situ cell deformation on calcium ion signaling in tendons under tensile load. Materials are tails from adult rat. The tendons were harvested from the tail. Fluo-3 AM was used as a indicator of the cytosolic calcium ion. Laser scanning confocal microscope was employed to detect the fluorescence. The cells were deformed and amount of cytosolic calcium ion increased as indicated by fluorescence of Fluo-3 under tensile load. Changes of cytosolic concentrations of calcium ion have been associated with several cell processes including DNA synthesis. The results of this study have demonstrated that tissue strain and resultant in situ cellar deformation causes a significant increase in the cytosolic levels of calcium ion.

机械环境对结缔组织动态平衡的重要性是众所周知的。虽然已知这种环境的变化会影响这些组织的生化和生物材料特性，但细胞介导机械应力的确切机制仍不清楚。本研究旨在探讨在拉伸载荷作用下，肌腱细胞原位变形对钙离子信号的影响。材料为成年大鼠的尾巴。肌腱是从尾巴上采集的。用Fluo-3 AM作为细胞内钙离子的指示剂。用激光扫描共聚焦显微镜检测荧光。在拉伸载荷下，Fluo-3的荧光显示细胞变形，胞内钙离子含量增加。细胞内钙离子浓度的变化与包括DNA合成在内的几个细胞过程有关。这项研究的结果表明，组织应变和随之而来的细胞原位变形导致细胞内钙离子水平显著增加。