Investigation of stretch activated calcium signaling in tenocytes in situ

点击翻译按钮获取中文摘要

The importance of the mechanical environment to homeostasis of connective tissue is well known. While alterations in this environment are known to affect the biochemical and biomaterial characteristics of these tissue, the exact mechanism by which mechanical stress is mediated by the cell is still unclear. The current study investigates the effect of in situ cell deformation on calcium ion signaling in tendons under tensile load, Materials are tails from adult rat. The tendons were harvested from the tail. Fluo-3 AM was used as a indicator of the cytosolic calcium ion. Laser scanning confocal microscope was employed to detect the fluorescence. The cells were deformed and amount of cytosolic calcium ion increased as indicated by fluorescence of Fluo-3 under tensile load. Changes of cytosolic concentrations of calcium ion have been associated with several cell processes including DNA synthesis. The results of this study have demonstrated that tissue strain and resultant in situ cellar deformation causes a significant increase in the cytosolic levels of calcium ion.