Investigation of stretch activated calcium signaling in tenocytes in situ

原位肌腱细胞拉伸激活钙信号传导的研究

• 批准号: 08671637
• 负责人: SHIRAKURA Kenji
• 金额: $ 1.47万
• 依托单位: Gunma University Faculty of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671637/
• 关键词: Calcium ion signaling; stretch activated ion channel; fluo-3-acetoxymethyl; rat; tenocyte; カルシウムイオンチャンネル; 張力依存性イオンチャンネル; Fluo-3 acetoxymethyl; 腱線維芽細胞; Fluo3-acetoxymethyl

The importance of the mechanical environment to homeostasis of connective tissue is well known. While alterations in this environment are known to affect the biochemical and biomaterial characteristics of these tissue, the exact mechanism by which mechanical stress is mediated by the cell is still unclear. The current study investigates the effect of in situ cell deformation on calcium ion signaling in tendons under tensile load, Materials are tails from adult rat. The tendons were harvested from the tail. Fluo-3 AM was used as a indicator of the cytosolic calcium ion. Laser scanning confocal microscope was employed to detect the fluorescence. The cells were deformed and amount of cytosolic calcium ion increased as indicated by fluorescence of Fluo-3 under tensile load. Changes of cytosolic concentrations of calcium ion have been associated with several cell processes including DNA synthesis. The results of this study have demonstrated that tissue strain and resultant in situ cellar deformation causes a significant increase in the cytosolic levels of calcium ion.

机械环境对结缔组织稳态的重要性是众所周知的。虽然已知这种环境的改变会影响这些组织的生物化学和生物材料特性，但细胞介导机械应力的确切机制仍不清楚。本研究旨在探讨拉伸载荷下肌腱原位细胞变形对钙离子信号的影响。肌腱是从尾巴上取下来的。Fluo-3 AM被用作细胞溶质钙离子的指示剂。激光扫描共聚焦显微镜检测荧光。在拉伸负荷下，Fluo-3的荧光显示细胞变形，胞浆钙离子量增加。细胞内钙离子浓度的变化与包括DNA合成在内的几种细胞过程有关。本研究的结果表明，组织应变和由此产生的原位细胞变形导致细胞内钙离子水平的显着增加。

项目成果

白倉,賢二: "ストレッチが結合組織に及ぼす影響・ラット腱線維芽細胞におけるカルシウムイオンチャンネルの観察・" 日本リハビリテーション医学会誌. 印刷中.

Kenji Shirakura：“拉伸对结缔组织的影响：大鼠肌腱成纤维细胞中钙离子通道的观察”日本康复医学会杂志正在出版。

白倉賢二,寺内正憲,柳沢治: "ストレッチが結合組織に及ぼす影響ラット線維芽細胞におけるカルシウムイオンチャンネルの観察" リハビリテーション医学. Vol.35(11). 820-821 (1999)

Kenji Shirakura、Masanori Terauchi、Osamu Yanagisawa：“拉伸对大鼠成纤维细胞钙离子通道观察的影响”康复医学第 35 卷（11）。

Kenji Shirakura et al.: "Observation of stretch activated calcium ion channel of the tenocyte in situ" Japanese Journal of Rehabilitation Medicine. 35(11). 820-821 (1998)

Kenji Shirakura 等人：“肌腱细胞原位拉伸激活钙离子通道的观察”日本康复医学杂志。

K.Shirakura: "Deformation Induced Calcium Signaling In Tenocyte In Situ" Transact.of 2nd Combined Meeting of ORS.94- (1995)

K.Shirakura：“原位肌腱细胞中变形诱导的钙信号传导”Transact.of 第二次 ORS.94 联合会议- (1995)