The molecular effect of general anesthesia on alveolar macrophages

全身麻醉对肺泡巨噬细胞的分子效应

基本信息

  • 批准号:
    06671500
  • 负责人:
  • 金额:
    $ 0.45万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

HUMAN STUDY : We studied patients anesthetized with propofol (n=30) or isoflurane (n=30) during major orthopedic surgery. Alveolar macrophages were harvested by bronchoalveolar lavage immediately, and 2,4, and 6 hours after induction anesthesia and at the end of surgery. The fraction of aggregated and nonviable macrophages was determined. We then measured phagocytosis by ingestion of opsonized and unopsonized particles. Finally, microbicidal activity was determined as the ability of the macrophages to directly kill Listeria monocytogenes.The fraction of alveolar macrophages ingesting opsonized and non-opsonized particles, and the number of particles ingested, decreased significantly over time, with the decrease being slightly but significantly greater during isoflurane anesthesia. Microbicidal function progressively decreased during anesthesia and surgery, with the decrease being almost twice as great during isoflurane than propofol anesthesia. The fraction of aggregated macrophages and recovered neutrophils increased over time in the patients given each anesthetic.ANIMAL STUDY : Rats received a tracheostomy followed by mechanical ventilation. We performed bronchoalveolar lavage to harvest AMs at the following time points : after two-hour mechanical ventilation without inhaled anesthetics, after two-hour mechanical ventilation with 1-MAC isoflurane, and after two-hour mechanical ventilation with 1-MAC halothane. We extracted RNAs and cDNA was synthesized with reverse transcriptase. We used specific 3'- and 5'-primers for four cytokines : IL-1alpha and beta, IL-6, IFN and TNF-alpha and macrophage inflammatory protein-2 (MIP-2). Before the experiment, we established the PCR condition that resulted in exponential amplification. For all cytokines, gene expression of all cytokines was minimal after two-hour mechanical ventilation without anesthetics. We found gene expression of IL-1, IFN,and TNF two hours after mechanical ventilation with isoflurane.
人体研究:我们研究了在大型骨科手术中使用丙泊酚(n=30)或异氟烷(n=30)麻醉的患者。分别于麻醉诱导后即刻、2、4、6 h及手术结束时行支气管肺泡灌洗,收集肺泡巨噬细胞。测定聚集的和无活力的巨噬细胞的分数。然后,我们通过摄取调理和未调理的颗粒来测量吞噬作用。最后,杀微生物活性被确定为巨噬细胞直接杀死单核细胞增生李斯特菌的能力。肺泡巨噬细胞吞噬经调理和未经调理的颗粒的分数,以及摄入的颗粒数量,随着时间的推移显著下降,在异氟烷麻醉期间下降幅度较小但显著更大。在麻醉和手术期间,杀微生物功能逐渐下降,异氟烷麻醉期间的下降幅度几乎是丙泊酚麻醉期间的两倍。聚集的巨噬细胞和恢复的中性粒细胞的分数增加,随着时间的推移,给予每一个anesthesies.Animal研究:大鼠接受气管切开术,然后机械通气。我们在以下时间点进行支气管肺泡灌洗以收获AM:在不使用吸入麻醉剂的机械通气2小时后,在使用1-MAC异氟烷的机械通气2小时后,以及在使用1-MAC氟烷的机械通气2小时后。我们提取RNA,用逆转录酶合成cDNA。我们使用了4种细胞因子的特异性3 '-和5'-引物:IL-1 α和β、IL-6、IFN和TNF-α以及巨噬细胞炎性蛋白-2(MIP-2)。在实验之前,我们建立了导致指数扩增的PCR条件。对于所有细胞因子,所有细胞因子的基因表达是最小的两个小时的机械通气后,没有麻醉剂。我们发现IL-1,IFN和TNF的基因表达后两个小时的机械通气异氟醚。

项目成果

期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kotani N,et al.: "Gene expression of cytokines in rat alveolar macrophages during anesthesia." Anesthesiology. 87. 1093-1093 (1998)
Kotani N 等人:“麻醉期间大鼠肺泡巨噬细胞中细胞因子的基因表达。”
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    0
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Kotani N, et al.: "Gene expression of cytokines in human alveolar macrophages during anesthesia and surgery" Anesthesiology. 87. 275-275 (1998)
Kotani N 等人:“麻醉和手术期间人肺泡巨噬细胞中细胞因子的基因表达”麻醉学。
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    0
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Kotani N, et al.: "Volatile anesthetics increase gene expression of cytokines in alveolar macrophages" Anesthesiology. 87. 366-366 (1998)
Kotani N 等人:“挥发性麻醉剂增加肺泡巨噬细胞中细胞因子的基因表达”麻醉学。
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    0
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kotani N,et al.: "a rapid increase in foot temperature predicts cardiovascular collapse during anaphylactic and anaphylactoid reaction." Anesthesiology. 559-568 (1997)
kotani N 等人:“在过敏和类过敏反应期间,足部温度的快速升高预示着心血管衰竭。”
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    0
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Kotani N: "Increased interlekin-1 secretion during general anesthesia" Anesthesia and Analgesia. 80. 278- (1995)
Kotani N:“全身麻醉期间 interlekin-1 分泌增加”麻醉和镇痛。
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    0
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KOTANI Naoki其他文献

KOTANI Naoki的其他文献

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{{ truncateString('KOTANI Naoki', 18)}}的其他基金

Research of accelerated method for reinforcement learning and its evaluation using redundant system with high-dimensional input
高维输入冗余系统强化学习加速方法及其评估研究
  • 批准号:
    16K16118
  • 财政年份:
    2016
  • 资助金额:
    $ 0.45万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Application of gene therapy to acute lung injury
基因治疗在急性肺损伤中的应用
  • 批准号:
    08457399
  • 财政年份:
    1996
  • 资助金额:
    $ 0.45万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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