THE ISCHEMIC CHANGES IN THE MITOCHONDRIA CALCIUM CONCENTRATION IN THE RAT BRAIN, HEART, AND LIVER

细胞内钙主要储存在线粒体和SR中。SR中的钙用于控制稳态时细胞内钙浓度。缺血状态下钙浓度的临界增加被认为是由线粒体的钙释放引起的。因此，我们研究了缺血状态下线粒体内钙浓度的变化，以观察缺血细胞损伤的初始变化。我们尝试分别按照Hogeboom的方法、Palmer的方法和Inoue的方法分离大鼠脑、心脏和肝脏的线粒体，并使用钙荧光剂测量缺血状态下的细胞内钙浓度。然而，不幸的是，我们不能分离足够的纯线粒体样品以具有高呼吸活性并被这些荧光剂染色。这可能是由于我们采用的线粒体分离方法，以及由于我们不熟练的处理。进一步的研究必须进行，以阐明在线粒体中的钙的初始缺血性变化。

The intracellular calcium is stored mostly in the mitochondria and SR. The calcium in SR is utilized for the control of the intracellular concentration of calcium in the steady-state. The critical increase in calcium concentration in the ischemic state is thought to be induced by the calcium release from mitochondria. Therefore, we investigate the change in calcium concentration in mitochondria in the schemic state in order to observe the initial change in the ischemic cellular damage.We tried to separate mitochondria from rat brain, heart, and liver, according to Hogeboom's method, Palmer's method, and Inoue's method, respectively, and to measure the intracellular calcium concentration in the ischemic condition by using calcium fluorescent agents.However, we were not able to separate pure mitochondrial samples enough to have high respiratory activity and to be stained by these fluorescent agents unfortunately. This might be due to the mitochondria separation methods we had employed, and due to our unskillful treatments. Further investigations have to be performed to elucidate the initial ischemic change of calcium in mitochondria.