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Clinical study on immunological causative background in repeated unsuccess of In Vitro fertilization and embryo transfer (IVF-ET)

体外受精胚胎移植（IVF-ET）多次失败的免疫学病因背景临床研究

基本信息

批准号：
06671668
负责人：
KANAZAWA Koji
金额：
$ 1.34万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671668/
关键词：
IVF-ET repeated unsuccess immunological causative background steroid danazol therapy ダナゾール療法

项目摘要

This study was focused on clinical analysis of immunologcal causative backgrounds in women with a history of repeated unsuccess of IVF-ET.The results were as follows : (1) A detection rare of micro-organisms in culture of vaginal fluor was significantly higher in the pregnant women treated with IVF-ET than in control group. (2) A positive rate of autoantibodies (antiphospholipid antibodies, lupus anticoagulant, rheumatoid factors, etc.) was significantly higher in the IVF-ET received women than in control group. (3) An incidence of elevated serum immunoglobulin (IgG/IgA/IgM) levels and/or of declined serum complement (C3/C4/CH50) titers were significantly higher in the IVF-ET group than in control group. (4) The majority of the autoantibody-positive women were affected by bacterial vaginosis. (5) Immuno-suppressive therapy using steroid hormone (p.o.) or danazol (p.o.) was applied to the women with a history of repetitive failure of IVF-ET.The trial brought a rise of a success rate of IVF-ET with improvement of abnormal immunologic findings. (6) Anticoagulant therapy with heparin (i.v.) resulted in a successful continuation of pregnancy in the women of habitual early abortion.These data suggests that immunological disorder with production of various autoantibodies can contribute to unsuccess of IVF-ET.As a next step, analysis on In Vitro polyclonal B-cell activation is indispensable to examine an interaction between infection of genital tract and production of autoantibodies in IVF-ET received women.

本研究对有反复IVF-ET失败史的妇女进行免疫学背景的临床分析，结果表明：（1）IVF-ET治疗组阴道分泌物培养菌群检出率明显高于对照组; (2)自身抗体阳性率（抗磷脂抗体、狼疮抗凝剂、类风湿因子等）IVF-ET组患者的血清总胆红素水平明显高于对照组。(3)IVF-ET组血清免疫球蛋白（IgG/伊加/IgM）水平升高和/或血清补体（C3/C4/CH 50）滴度下降的发生率显著高于对照组。(4)大多数自身抗体阳性的妇女受到细菌性阴道病的影响。(5)使用类固醇激素的免疫抑制疗法（p.o.）或达那唑（p.o.）对有IVF-ET反复失败史的妇女进行了IVF-ET治疗，提高了IVF-ET的成功率，改善了免疫学异常。(6)肝素抗凝治疗（i. v.）这些数据表明，免疫紊乱与各种自身抗体的产生可能导致IVF-ET失败。作为下一步，体外多克隆B细胞活化分析是必不可少的，以研究生殖道感染和IVF-ET接受妇女自身抗体产生之间的相互作用。