Significance of HPV DNA/mRNA in metastasissuspected tissues in carcinoma of the cervix : A prospective observational study

宫颈癌转移疑似组织中 HPV DNA/mRNA 的意义：一项前瞻性观察研究

• 批准号: 09671703
• 负责人: KANAZAWA Koji
• 金额: $ 1.41万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671703/
• 关键词: carcinoma of the cervix; HPV DNA; diagnosis of metastasis; prospective study; mRNA

Objective : to prospectively clarify the clinical significance of HPV DNA in nodal and other tissues, with special reference to its possible determinant in diagnosing occult metastasis in cervical cancer.Methods : 293 who were tested for HPV DNA of primary tumor before initiation of treatment, were prospectively analysed for a possible correlation between presence or absence of HPV DNA and histological findings in metastasis-suspected tissue samples. The polymerase chain reaction was performed using fresh tissue or cell specimens, immediately after sampling.Results : In 250 of 293 patients (85.3%), HPV DNA was detected in cervical tumor tissues. The positive rate by histology was 89.9% in squamous cell carcinoma, 93.8% in adenosquamous carcinoma and 51.4% in adenocarcinoma, and the former two figures were significantly higher than the latter (p<O.OO1 and p=O.OO2). HIPV type 16 was most predominant in squamous cell carcinoma, while type 18 was relatively high prevalent in adenocarcinoma … More . 52% were not determined for HPV types. After start of treatment, in 489 nodal and other tissue samples which were obtained from 113 of 250 HPV DNA-positive patients, HPV DNA was amplified in all of 55 samples metastasized and even in 12 of 434 not metastasized (2.8%). HPV types specified in these samples were always identical with the HPV types determined in their primary tumors. In 154 samples from 29 of 43 HPV DNA-negative patients, HPV DNA was not detected in both sapmles metastasized and not metastasized. Cancer-free but HPV DNA-positive tissues could be interpreted to be already involved at the time of examination, by checking them with clinical courses of the disease.Conclusions : in carcinoma of the cervix, oncogenic HPV DNA analysis of nodes and other tissues may be a useful adjunct to routine microscopic evaluation, possibly as a determinant of occult metastasis. Patietns who have tumor-free but HPV DNA-positive samples should be planned to receive some treatment for the sample sites and also monitored as a possible high-risk group of recurrence. Less

目的：方法：前瞻性分析293例宫颈癌原发灶治疗前HPV DNA检测结果，并对可疑转移灶组织中HPV DNA的检出与病理学检查结果进行相关性分析。结果：293例宫颈癌患者中，250例（85.3%）宫颈癌组织中HPV DNA阳性。鳞癌、腺鳞癌和腺癌的阳性率分别为89.9%、93.8%和51.4%，前两者明显高于后者（p<0.001和p= 0.002）。HIPV 16型在鳞状细胞癌中最常见，而18型在腺癌中相对较高 ...更多信息 . 52%未确定HPV类型。治疗开始后，在从250例HPV DNA阳性患者中的113例获得的489个淋巴结和其他组织样本中，HPV DNA在所有55个转移的样本中扩增，甚至在434个未转移的样本中有12个（2.8%）扩增。在这些样本中指定的HPV类型总是与在其原发性肿瘤中确定的HPV类型相同。在43例HPV DNA阴性的患者中，29例的154份标本中，转移和未转移的标本均未检测到HPV DNA。无癌，但HPV DNA阳性组织可以解释为已经参与检查时，通过检查他们的临床病程的diseases.Conclusions：在宫颈癌，致癌HPV DNA分析的节点和其他组织可能是一个有用的辅助常规显微镜评价，可能作为一个决定因素的隐匿性转移。无肿瘤但HPV DNA阳性样本的患者应计划接受样本部位的一些治疗，并作为可能的复发高危人群进行监测。少

项目成果

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