Prevalence of HPV infection in women from Okinawa: Is high-risk HPV infection a potential factor in carcinoma of the cervix?

冲绳女性 HPV 感染率：高危 HPV 感染是宫颈癌的潜在因素吗？

• 批准号: 12671618
• 负责人: KANAZAWA Koji
• 金额: $ 2.37万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671618/
• 关键词: HPV; potential factor; carcinoma of the cervix; HPV infection; prevalence; cervical carcinoma; Okinawa

[Objective]: To analyze the prevalence of human papillomavirus (HPV) infection in a large number of women with cytologically normal cervix and patients with dysplasia or carcinoma of the cervix from Okinawa.[Methods]: From 1994 to 1999, in a total of approximately 4800 subjects, the swabbed cells from the cervix were smeared on glass-slides for light microscopy, and cell suspensions were made for HPV DNA assay. HPV DNA assay by PCR was performed independent of histologic examination. L1 consensus primer was employed for HPV DNA detection. For the HPV DNA positive samples, the PCR for identification of HPV types was performed using the primers including HPV type 16 and 18.[Results]: 1) HPV DNA was positive in 10.6% (434/4078 cases) of cervical cytology-normal women and the detection rate of type 16/18 was only 3.0% (13/434 cases). 2) HPV DNA was positive in 64.2% (102/159 cases) of patients with cervical dysplasia and in 85.6% (415/485 cases) of those with cervical carcinoma. Type 16/18 was identified in 15.7% (16/102 cases) of the former group and in 31.3% (152/415 cases) of the latter. Thus, the positive rate of HPV DNA and of type 16/18 were.significantly higher in the dysplasia or carcinoma patients than in the cervix-normal women (p<0.001), and also significantly higher in the carcinoma patients than in the dysplasia (p<0.001).[Conclusion]: It was strongly suggested that HPV, especially high-risk type HPV is a potential factor in the carcinogenesis of cervical carcinoma through this crosssectional analysis.

【目的】：分析冲绳地区宫颈细胞学正常的大量女性及宫颈发育不良或癌患者的人乳头瘤病毒（HPV）感染情况。【方法】：1994年至1999年，共约4800名受试者，将宫颈拭子细胞涂片于玻片上进行光镜观察，并制作细胞悬液进行HPV DNA检测。HPV DNA PCR检测独立于组织学检查。采用L1一致引物进行HPV DNA检测。对于HPV DNA阳性样本，使用HPV 16型和18型引物进行PCR鉴定HPV型别。【结果】：1)宫颈细胞学正常女性HPV DNA检出率为10.6%（434/4078例），16/18型检出率仅为3.0%（13/434例）。2)宫颈发育不良患者HPV DNA阳性率为64.2%（102/159例），宫颈癌患者阳性率为85.6%（415/485例）。前一组16/18型占15.7%（16/102例），后一组为31.3%（152/415例）。因此，HPV DNA和16/18型的阳性率分别为。宫颈异常增生和癌组明显高于宫颈正常组（p<0.001），癌组明显高于宫颈异常增生组（p<0.001）。【结论】：通过本次横断面分析，强烈提示HPV，特别是高危型HPV是宫颈癌发生的潜在因素。

项目成果

Nagai Y, Maehama T, Kanazawa K, et al.: "Detection of human papillomavirus DNA in primary and metastatic lesions of carcinoma of the cenvix in women from Okinawa, Japan"Am J Clin Oncol. 24. 160-166 (2001)

Nagai Y、Maehama T、Kanazawa K 等人：“日本冲绳女性宫颈癌原发性和转移性病变中人乳头瘤病毒 DNA 的检测”Am J Clin Oncol。

Nagai Y., Maehama T., Kanazawa K., et al: "Detection of human papillomavirus DNA in primary and metastatic lesions of carcinoma of the cervix in women from Okinawa, Japan."Am J. Clin. Oncol.. 24. 160-166 (2001)

Nagai Y.、Maehama T.、Kanazawa K. 等人：“日本冲绳女性宫颈癌原发性和转移性病变中人乳头瘤病毒 DNA 的检测。”Am J. Clin。

Nagai Y Maehama T, Kanazawa K et al.: "Persistence of human papillomavirus infection after therapeutic conization for CIN3 : is it an alarm for disease recurrence ?"Gynecol Oncol. 79. 294-299 (2000)

Nagai Y Maehama T、Kanazawa K 等人：“CIN3 锥切治疗后人乳头瘤病毒感染的持续存在：这是疾病复发的警报吗？”Gynecol Oncol。

前濱俊之, 金澤浩二: "HPV感染の疫学と自然史"産婦実験. 49. 643-646 (2000)

Toshiyuki Maehama、Koji Kanazawa：“HPV 感染的流行病学和自然史”母体实验 49. 643-646 (2000)。

Maehama T,Asato T,Kanazawa K: "Prevalence of HPV infection in cervical cytology-normal women in Okinawa, Japan, as determined by a polymerase chain reaction."International Journal of Gynecology Obstetrics. 69. 175-176 (2000)

Maehama T、Asato T、Kanazawa K：“通过聚合酶链反应确定日本冲绳宫颈细胞学正常女性 HPV 感染的患病率。”国际妇产科杂志。