Detection and analysis of a factor concerned with cell differentiation and growth during mouse molar tooth development by means of the grafting to a chick wing bud.

通过移植到鸡翼芽上来检测和分析与小鼠磨牙发育过程中的细胞分化和生长有关的因素。

• 批准号: 06671810
• 负责人: YAMAAI Yuichiro
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671810/
• 关键词: Mouse; Tooth germ; ZPA; Grafting; Chick limb bud; In situ hybridization; モルフォゲン; 移植; 重復肢誘導; マウス; 歯胚; 間葉

Tooth development involves reciprocal epithelio-mesenchymal interactions and complex morphogenetic events. Because these processes bear similarities to those occurring in the developing limb, we asked whether signaling mechanisms found in the limb also occur in the tooth. We grafted mouse embryonic tooth germs to the anterior margin of the host chick embryonic wing buds and determined whether the tooth tissues had polarizing activity. Indeed the grafted tooth germs induced the formation of the ectopic digits as a miller image as well as the surface structures. These activity of the molar tooth germ increased from bud to cap stage and reached at the maximum at late bell stage. With further development, the polarizing activity began to decrease, however, once it became the highest level at neonates. And then the activity decreased to undetectable level at 3-week old.In situ hybridization demonstrated that the tooth germ grafts induced expression of chick HoxD cluster genes prior to induction of ectopic digits. We concluded that the factors produced by the tooth germs were similar to those in the zone of polarizing activity in the limb development and might regulate morphogenesis and differentiation in the molar tooth development.

牙齿发育涉及相互的上皮间质相互作用和复杂的形态发生事件。由于这些过程与发育中的肢体中发生的过程相似，我们想知道在肢体中发现的信号机制是否也发生在牙齿中。我们将小鼠胚胎牙胚移植到宿主鸡胚胎翼芽的前缘，并测定牙齿组织是否具有极化活性。事实上，移植的牙胚诱导了异位手指的形成，如米勒图像以及表面结构。磨牙牙胚的这些活性从芽期到冠期逐渐增加，并在钟形后期达到最大。随着进一步的发展，极化活动开始下降，然而，一旦达到新生儿的最高水平。然后在3周龄时活性下降至不可检测的水平。原位杂交表明，牙胚移植物在异位指诱导之前诱导了鸡HoxD簇基因的表达。我们得出的结论是，牙胚产生的因子与肢体发育中极化活动区的因子相似，可能调节臼齿发育中的形态发生和分化。