Ion channel analysis of neurons of the mesencephalic trigeminal nucleus in in vitro preparations

体外制剂中中脑三叉神经元的离子通道分析

• 批准号: 06671850
• 负责人: KATAKURA Nobuo
• 金额: $ 1.28万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671850/
• 关键词: trigeminal mesencephalic nucleus; slice preparation; isolated brainstem-spinal cord preparation; ion-channel; rhythm; 舌活動; 吸啜運動; スライス標本; 閉口筋; 筋紡錘; 歯根膜機械受容器; 多極性ニューロン; 偽単極性ニューロン

In the trigeminal mesencephalic nucleus (MTN), there are two functional types of neurons : one supplying the muscle spindles in jaw-closing muscles and the other the periodontal mechanoreceptors, and also two anatomical types of neurons : pseudomonopolar and multipolar. The purpose of this study was to investigate the organization of ion-channels on each type of MTN neurons in in vitro preparations.To achieve this goal, I had developed two types of in vitro preparations : slice preparation and isolated brainstem-spinal cord preparation. Although the slice preparation is of use to an analysis of ion-channels, it will not achieve characterizations of individual cells and a system analysis. On the other hand, the isolated brainstem-spinal cord preparation involves intact circuits including MTN neurons and generates reflex responses and rhythmical activity.The in vitro brainster-spinal cord preparation were isolated from neonatal rats (from 0- to 3-day-old). Neural activities were monitored in trigeminal sensory (Vs), trigeminal motor (Vm), hypoglossal (Xll) nerves as well as ventral roots of the cervical spinal cord (C4-C5) with suction electrodes. The following results were obtained : (1) electrical stimulation of Vs evoked nerve volley in Vm and Xll ; (2) rhythmical nerve discharges distinct from spontaneously generated respiratory activities recorded in Vm, Xll and C4,5 nerves could be observed in the Xll nerve after application of N-menthyl-D-aspartate into the bath solution.I conclude that this isolated brainstem-spinal cord preparation will be useful for the investigation of cellular mechanisms (ion-channel) and neuronal organization (system) related to the function of MTN neurons. At present, I am trying to analyze ion-channel mechanisms of MTN neurons in these preparations.

在三叉神经中脑核（MTN），有两种功能类型的神经元：一个供应肌肉梭在下颌闭合肌肉和其他牙周机械感受器，也有两种解剖类型的神经元：假单极和多极。本研究的目的是在离体标本中研究MTN神经元上离子通道的组织结构，为此，我研制了两种类型的离体标本：切片标本和离体脑干-脊髓标本。虽然切片制备用于离子通道的分析，但它不能实现单个细胞的表征和系统分析。另一方面，分离的脑干-脊髓制备物涉及包括MTN神经元在内的完整回路，并产生反射反应和节律活动。用抽吸电极监测三叉神经感觉（Vs）、三叉神经运动（Vm）、舌下神经（Xll）以及颈脊髓（C4-C5）的前根的神经活动。结果表明：（1）电刺激Vs可引起Vm和X11的神经放电;（2）在Vm、X11和C4中记录到与自发产生的呼吸活动不同的节律性神经放电，将N-薄荷基-D-天冬氨酸应用于浴液中后，在X11神经中可观察到5条神经。脊髓标本将有助于研究与MTN神经元功能相关的细胞机制（离子通道）和神经元组织（系统）。目前，我正试图在这些制剂中分析MTN神经元的离子通道机制。

项目成果

Katakura, N.: "Brain and Oral Functions Oral motor function and dysfunction" Morimoto, T., Matsuya, T., Takada, K., 648 (1995)

Katakura, N.：“大脑和口腔功能、口腔运动功能和功能障碍” Morimoto, T.、Matsuya, T.、Takada, K.，648 (1995)

Katakura, N., Sahara, Y.and Nakamura, Y: "Pharmacologically induced rhythmical activities in the hypoglossal nerve of isolated brainstem-spinal cord preparation from neonatal rats." Osaka International Oral Physiology Symposium Abstracts. 56. (1994)

Katakura, N.、Sahara, Y. 和 Nakamura, Y：“新生大鼠离体脑干脊髓制剂的舌下神经中药理学诱导的节律活动。”

Katakura,N.: "NMDA-induced rhythmical activities of the hypoglossal motoneurons in an in vitro brainstem-spinal cord preparation from newborn rats." Neurosci.Res. Suppl.19. S176 (1994)

Katakura,N.：“在新生大鼠的体外脑干脊髓制剂中，NMDA 诱导舌下运动神经元的节律活动。”

Katakura, N., Lia J.and Nakamura, Y: "NMDA-induced sucking-like activity in isolated brainstem-spinal cord preparation from newborn rats." The Second Joint Meeting of The Physiological Societies of Japan and U.K.and Eire ; Okazaki Symposium Abstracts. 132

Katakura, N.、Lia J. 和 Nakamura, Y：“新生大鼠离体脑干脊髓制剂中 NMDA 诱导的吸吮样活动。”

Katakura, N., Lia J.and Nakamura, Y: "NMDA-induced rhythmical activity in Xll nerve of isolated CNS from newborn rats." Neuro Report. 6. 601-604 (1995)

Katakura, N.、Lia J. 和 Nakamura, Y：“NMDA 诱导新生大鼠离体中枢神经系统 XII 神经节律活动。”