权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Functional relationships between Ca-ion channels and adrenergic receptors for protein secretion

钙离子通道与肾上腺素能受体之间蛋白质分泌的功能关系

基本信息

批准号：
06671874
负责人：
ABE Kimio
金额：
$ 1.34万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The functional relationships between Ca-ion channels and various autonomic receptors in the rat submandibular glands were studied by using various agonist with four kinds of Ca-ion channel blockers (CdCl_2, verapamil, nifedipine and diltiazem). The Ca-ion channel was greatly involved in fluid and protein secretion by the Salivary glands of rats. However, the mode of its physiological actions was quite complicated depending upon the combination of the agonists and Ca-ion channel blockers. Fluid and protein secretion in response to several agonists was significantly accerelated or was significantly inhibited due to combination modes between Ca-blockers and agonists. The actions of Ca-blockers were thus compared to those of reserpine, which had noradrenaline release via autonomic nerve terminals. For fluid and protein secretion, two drugs were very similar each other, but for the catecholamine behavior, they were quite different. Therefore, three catecholamine contents were determined in various tissue homogenates. The level of adrenaline, but not for dopamine and noradrenaline, was significantly affected with a Ca-blocker, especially in the salivary gland. Furthermore, alphal- and betal-adrenergic receptor responses in the salivary gland were argumented with a Ca-blocker. However, further studies are necessary to elucidate how Ca-blockers act and interact with cholinergic and adrenergic receptors and how ca-ion channels are involved in fluid and protein secretion by the salivary glands.

用不同的激动剂与四种钙离子通道阻滞剂(氯化镉、维拉帕米、硝苯地平和地尔硫卓)合用，研究了大鼠颌下腺钙离子通道与各种自主神经受体的功能关系。钙离子通道是大鼠唾液腺分泌液体和蛋白质的重要通道。然而，其生理作用模式相当复杂，依赖于激动剂和钙离子通道阻滞剂的组合。由于钙拮抗剂和激动剂之间的结合方式，对几种激动剂的液体和蛋白质分泌显著加速或显著抑制。因此，钙阻滞剂的作用与利血平的作用进行了比较，利血平通过自主神经末梢释放去甲肾上腺素。对于液体和蛋白质的分泌，两种药物非常相似，但对儿茶酚胺的行为，它们有很大的不同。因此，测定了不同组织匀浆中三种儿茶酚胺的含量。钙阻滞剂显著影响肾上腺素水平，但不影响多巴胺和去甲肾上腺素水平，尤其是唾液腺。此外，唾液腺中的α-和β-肾上腺素能受体反应也用钙阻滞剂进行了论证。然而，需要进一步的研究来阐明钙阻滞剂如何作用并与胆碱能和肾上腺素能受体相互作用，以及钙离子通道如何参与唾液腺分泌液体和蛋白质。