Functional relationships between Ca-ion channels and adrenergic receptors for protein secretion

采用多种激动剂（CdCl_2、维拉帕米、硝苯地平、地尔硫平）联合四种钙离子通道阻滞剂，研究了大鼠颌下腺钙离子通道与多种自主神经受体的功能关系。钙离子通道在很大程度上参与了大鼠唾液腺的液体和蛋白质分泌。然而，它的生理作用模式是相当复杂的，取决于激动剂和钙离子通道阻滞剂的组合。由于钙受体阻滞剂和激动剂的联合模式，对几种激动剂反应的液体和蛋白质分泌显着加速或显着抑制。因此，钙受体阻滞剂的作用与利血平的作用进行了比较，后者通过自主神经末梢释放去甲肾上腺素。对于液体和蛋白质的分泌，两种药物彼此非常相似，但对于儿茶酚胺的行为，他们有很大的不同。因此，测定了不同组织匀浆中三种儿茶酚胺的含量。钙受体阻滞剂显著影响肾上腺素水平，但不影响多巴胺和去甲肾上腺素水平，尤其是唾液腺。此外，唾液腺α和β -肾上腺素能受体的反应与钙受体阻滞剂有关。然而，需要进一步的研究来阐明钙受体阻滞剂如何与胆碱能受体和肾上腺素能受体相互作用，以及钙离子通道如何参与唾液腺的液体和蛋白质分泌。

The functional relationships between Ca-ion channels and various autonomic receptors in the rat submandibular glands were studied by using various agonist with four kinds of Ca-ion channel blockers (CdCl_2, verapamil, nifedipine and diltiazem). The Ca-ion channel was greatly involved in fluid and protein secretion by the Salivary glands of rats. However, the mode of its physiological actions was quite complicated depending upon the combination of the agonists and Ca-ion channel blockers. Fluid and protein secretion in response to several agonists was significantly accerelated or was significantly inhibited due to combination modes between Ca-blockers and agonists. The actions of Ca-blockers were thus compared to those of reserpine, which had noradrenaline release via autonomic nerve terminals. For fluid and protein secretion, two drugs were very similar each other, but for the catecholamine behavior, they were quite different. Therefore, three catecholamine contents were determined in various tissue homogenates. The level of adrenaline, but not for dopamine and noradrenaline, was significantly affected with a Ca-blocker, especially in the salivary gland. Furthermore, alphal- and betal-adrenergic receptor responses in the salivary gland were argumented with a Ca-blocker. However, further studies are necessary to elucidate how Ca-blockers act and interact with cholinergic and adrenergic receptors and how ca-ion channels are involved in fluid and protein secretion by the salivary glands.