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Functional relationships between Ca-ion channels and adrenergic receptors for protein secretion

钙离子通道与肾上腺素能受体之间蛋白质分泌的功能关系

基本信息

批准号：
06671874
负责人：
ABE Kimio
金额：
$ 1.34万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The functional relationships between Ca-ion channels and various autonomic receptors in the rat submandibular glands were studied by using various agonist with four kinds of Ca-ion channel blockers (CdCl_2, verapamil, nifedipine and diltiazem). The Ca-ion channel was greatly involved in fluid and protein secretion by the Salivary glands of rats. However, the mode of its physiological actions was quite complicated depending upon the combination of the agonists and Ca-ion channel blockers. Fluid and protein secretion in response to several agonists was significantly accerelated or was significantly inhibited due to combination modes between Ca-blockers and agonists. The actions of Ca-blockers were thus compared to those of reserpine, which had noradrenaline release via autonomic nerve terminals. For fluid and protein secretion, two drugs were very similar each other, but for the catecholamine behavior, they were quite different. Therefore, three catecholamine contents were determined in various tissue homogenates. The level of adrenaline, but not for dopamine and noradrenaline, was significantly affected with a Ca-blocker, especially in the salivary gland. Furthermore, alphal- and betal-adrenergic receptor responses in the salivary gland were argumented with a Ca-blocker. However, further studies are necessary to elucidate how Ca-blockers act and interact with cholinergic and adrenergic receptors and how ca-ion channels are involved in fluid and protein secretion by the salivary glands.

用不同的钙通道激动剂和四种钙通道阻断剂（CdCl_2、维拉帕米、硝苯地平和地尔硫卓），研究了大鼠下颌下腺钙通道与植物神经受体的功能关系。钙离子通道在大鼠唾液腺的液体和蛋白质分泌中起重要作用。然而，其生理作用的模式是相当复杂的，这取决于激动剂和钙离子通道阻滞剂的组合。液体和蛋白质的分泌响应于几种激动剂是显着accerestrated或显着抑制，由于钙受体阻滞剂和激动剂之间的组合模式。因此，钙受体阻滞剂的行动进行了比较，利血平，通过自主神经末梢去甲肾上腺素释放。对于液体和蛋白质分泌，两种药物非常相似，但对于儿茶酚胺的行为，它们有很大的不同。因此，在各种组织匀浆中测定了三种儿茶酚胺含量。肾上腺素的水平，但不是多巴胺和去甲肾上腺素，显着影响与钙受体阻滞剂，特别是在唾液腺。此外，唾液腺中的β-和β-肾上腺素能受体反应与Ca-受体阻滞剂有关。然而，进一步的研究是必要的，以阐明钙受体阻滞剂的作用和相互作用与胆碱能和肾上腺素能受体和钙离子通道是如何参与唾液腺的液体和蛋白质分泌。