Purification and their physiological function of proteins as indicators of aging involved in adrenoceptors

研究并纯化了与大鼠肾上腺素受体生理功能有关的衰老指标蛋白质。和小鼠下颌下唾液。在MRL/1小鼠在老年，酸性和碱性蛋白质显着增加下颌下腺唾液。这些蛋白质的纯化，并确定其生化性质和N-末端氨基酸序列，然后在ddy小鼠下颌下腺唾液中进行同源性分析。酸性和碱性蛋白质，增加老年MRL/1小鼠下颌下唾液，半胱氨酸蛋白酶抑制剂的活动。酸性蛋白质和碱性蛋白质的等电点和分子量分别为4.6和9.1，以及12，900和14，500。这些蛋白可能也参与了ODU大鼠牙龈袋的形成，导致牙龈炎。需要进一步的研究来阐明淋巴细胞的分化和发育与这些半胱氨酸蛋白酶抑制剂之间的关系。

Some proteins, as indicators of aging, involving in the physiological functions of the adrenoceptor were investigated and purified in rat. and mouse submandibular saliva. In the MRL/1 mouse at the old age, acidic and basic proteins prominently increased in submandibular saliva. These proteins were purified and determined their biochemical properties and N-terminal amino acid sequences followed by their homology analysis in ddy mouse submandibular saliva. Acidic and basic proteins, increased in aged MRL/1 mouse submandibular saliva, had cystatin activities. The isoelectric points and molecular weights of an acidic and a basic proteins were 4.6 and 9.1, and 12,900 and 14,500, respectively. These proteins may be also involved in gingival pocket formation of the ODU rats which caused periodontitis in gingivae. Further studies are necessary to elucidate the relationships between differentiation and development of lymphocytes and these cystatins.