Purification and their physiological function of proteins as indicators of aging involved in adrenoceptors

肾上腺素受体相关蛋白质的纯化及其作为衰老指标的生理功能

• 批准号: 03670884
• 负责人: ABE Kimio
• 金额: $ 1.28万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670884/
• 关键词: Aging; Autonomic receptor; Salivary protein; MRL; 1 mouse; ODU rat; Cystatins; Abnormal immunity; Lymphocyte; Salivary gland; Agonist; Ageing; Salivary Gland; Saliva protein; Amino acid composition; Rat and mouse(MRL; Ipr); Postーtranslati

Some proteins, as indicators of aging, involving in the physiological functions of the adrenoceptor were investigated and purified in rat. and mouse submandibular saliva. In the MRL/1 mouse at the old age, acidic and basic proteins prominently increased in submandibular saliva. These proteins were purified and determined their biochemical properties and N-terminal amino acid sequences followed by their homology analysis in ddy mouse submandibular saliva. Acidic and basic proteins, increased in aged MRL/1 mouse submandibular saliva, had cystatin activities. The isoelectric points and molecular weights of an acidic and a basic proteins were 4.6 and 9.1, and 12,900 and 14,500, respectively. These proteins may be also involved in gingival pocket formation of the ODU rats which caused periodontitis in gingivae. Further studies are necessary to elucidate the relationships between differentiation and development of lymphocytes and these cystatins.

研究并纯化了与大鼠肾上腺素受体生理功能有关的衰老指标蛋白质。和小鼠下颌下唾液。在MRL/1小鼠在老年，酸性和碱性蛋白质显着增加下颌下腺唾液。这些蛋白质的纯化，并确定其生化性质和N-末端氨基酸序列，然后在ddy小鼠下颌下腺唾液中进行同源性分析。酸性和碱性蛋白质，增加老年MRL/1小鼠下颌下唾液，半胱氨酸蛋白酶抑制剂的活动。酸性蛋白质和碱性蛋白质的等电点和分子量分别为4.6和9.1，以及12，900和14，500。这些蛋白可能也参与了ODU大鼠牙龈袋的形成，导致牙龈炎。进一步的研究是必要的，以阐明淋巴细胞的分化和发育与这些半胱氨酸蛋白酶抑制剂之间的关系。

项目成果

Okina,A.,Abe,K.,Inuzuka,H.,Yano,T.,Okina,T.,et.al.: "The effects of m-octopamine on salivary flow rates and protein secretion by rat submandi-bular glands" Comparative Biochemistry and Physiol.103. 469-476 (1992)

Okina,A.,Abe,K.,Inuzuka,H.,Yano,T.,Okina,T.,et.al.：“m-章鱼胺对大鼠颌下腺唾液流速和蛋白质分泌的影响

Shibaike,S.Abe,K.,Okina,A.,Nishiura,T.: "The effects of clonidine and three 2-imidazoline derivatives on the secretion of protein and some electrolytes by rat submandibular---" Comparative Biochemistry and Physiology.101. 547-556 (1992)

Shibaike，S.Abe，K.，Okina，A.，Nishiura，T.：“可乐定和三种2-咪唑啉衍生物对大鼠颌下分泌蛋白质和一些电解质的影响——”比较生物化学和生理学。

翁淳二: "ラット顎下腺の分泌機能に及ぼす3種類のフェノールアミンの作用" 福岡歯科大学学会雑誌. 19. 75-100 (1992)

Junji Okina：“三种酚胺对大鼠颌下腺分泌功能的影响”福冈齿科大学学报19. 75-100（1992）。

Okina,A.,Abe,K.Tashiro,M.and Ishibashi,K.: "The effects of p-octopamine on salivary flow rates and protein secretion by rat submandibular glands" Journal of Dental Research. 72. 993-1000 (1993)

Okina，A.，Abe，K.Tashiro，M. 和 Ishibashi，K.：“p-章鱼胺对大鼠颌下腺唾液流速和蛋白质分泌的影响”牙科研究杂志。

Satoru Shibaike: "The effects of clonidine and three 2-imidazoline derivatives on the secretion of protein and some electrolytes by rat submandibular and parotid glands" Comp. Biochem. Physiol.101C(3). 547-556 (1992)

Satoru Shibaike：“可乐定和三种 2-咪唑啉衍生物对大鼠颌下腺和腮腺分泌蛋白质和一些电解质的影响”Comp。