Study of bioactivity and biological adhesion of bone-marrow derived Osteoblastic cells cultured on titanium surface

人工材料与周围骨组织之间的初始相互作用通过成骨细胞克隆TMS-12细胞的体外培养来研究。为了确定钛的生物学特性，研究了钛表面对TMS-12细胞的各种细胞功能的影响。TMS-12细胞在钛板上的增殖能力明显低于塑料培养板。然而，每种材料的早期细胞附着和碱性磷酸酶活性相等。此外，1 α，25-二羟维生素D_3还能显著抑制脾细胞与TMS-12细胞共培养诱导的骨细胞形成，而在钛表面则能显著促进TMS-12细胞产生前列腺素E_2。在非粘附条件下，钛表面维持TMS-12细胞粘附和含乙酰基-甘氨酰-乙酰基的基质蛋白同样参与细胞与每种材料的粘附。这些结果表明，TMS-12细胞与钛表面直接接触可诱导前列腺素E_2的大量产生，而不影响破骨细胞的形成，提示在钛表面成骨细胞增殖的减少可通过抑制破骨细胞的形成而得到补偿，前列腺素E_2可能作为成骨细胞紧密附着在钛表面而释放的内源性因子在骨代谢中发挥作用。

Initial interaction between the artificial materials and the surrounding bone tissue were investigated by means of in vitro cultures of osteoblastic clone TMS-12 cells. To determine the biological properties of titanium, the effect of titanium surface on various cell functions of TMS-12 cells was studied. On titanium surface, proliferation of TMS-12 cells was decreased significantly compared with that on plastic tissue culture plates. However, early cell attachment and alkaline phosphatase activity were equal is each material. Moreover, significant inhibition of osteoclastic cell formation induced by the coculture of spleen cells with TMS-12 cells in the presence of 1alpha, 25-dihydroxyvitamin D_3 was observed : prostaglandin E_2 production in TMS-12 cells, on the other hand, was markedly stimulated on titanium surface. Under non-adherent conditions, titanium surface maintained TMS-12 cell adhesion and arginyl-glycyl-aspartyl-containing matrix proteins are equally involved in cell attachment to each material. These results indicate that high production of prostaglandin E_2 is induced by direct contact of TMS-12 cells to titanium surface and does not affect on osteoclast formation, suggesting that on titanium surface decrease of osteoblast proliferation is compensated by inhibition of osteoclast formation and that prostaglandin E_2 may play a role in bone metabolism as endogenous factor of osteoblast released by tight attachment ot titanium surface.