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Study of bioactivity and biological adhesion of bone-marrow derived Osteoblastic cells cultured on titanium surface

钛表面培养骨髓成骨细胞的生物活性及生物粘附研究

基本信息

批准号：
06671996
负责人：
NAGURA Hideaki
金额：
$ 1.34万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Initial interaction between the artificial materials and the surrounding bone tissue were investigated by means of in vitro cultures of osteoblastic clone TMS-12 cells. To determine the biological properties of titanium, the effect of titanium surface on various cell functions of TMS-12 cells was studied. On titanium surface, proliferation of TMS-12 cells was decreased significantly compared with that on plastic tissue culture plates. However, early cell attachment and alkaline phosphatase activity were equal is each material. Moreover, significant inhibition of osteoclastic cell formation induced by the coculture of spleen cells with TMS-12 cells in the presence of 1alpha, 25-dihydroxyvitamin D_3 was observed : prostaglandin E_2 production in TMS-12 cells, on the other hand, was markedly stimulated on titanium surface. Under non-adherent conditions, titanium surface maintained TMS-12 cell adhesion and arginyl-glycyl-aspartyl-containing matrix proteins are equally involved in cell attachment to each material. These results indicate that high production of prostaglandin E_2 is induced by direct contact of TMS-12 cells to titanium surface and does not affect on osteoclast formation, suggesting that on titanium surface decrease of osteoblast proliferation is compensated by inhibition of osteoclast formation and that prostaglandin E_2 may play a role in bone metabolism as endogenous factor of osteoblast released by tight attachment ot titanium surface.

通过体外培养成骨克隆TMS-12细胞，研究了人工材料与周围骨组织的初始相互作用。为了确定钛的生物学特性，研究了钛表面对TMS-12细胞各项功能的影响。与塑料组织培养板相比，钛表面TMS-12细胞的增殖能力明显降低。然而，两种材料的早期细胞附着和碱性磷酸酶活性是相等的。此外，在1α， 25-二羟基维生素D_3存在的情况下，脾脏细胞与TMS-12细胞共培养诱导的破骨细胞形成明显受到抑制，另一方面，钛表面明显刺激TMS-12细胞中前列腺素E_2的产生。在非黏附条件下，钛表面维持TMS-12细胞黏附，含精氨酸酰-甘氨酸-天冬氨酸的基质蛋白同样参与细胞对每种材料的黏附。这些结果表明，TMS-12细胞与钛表面直接接触可诱导前列腺素E_2的大量产生，而不影响破骨细胞的形成，提示在钛表面，成骨细胞增殖的减少可通过抑制破骨细胞的形成来补偿，前列腺素E_2可能作为成骨细胞紧密附着于钛表面释放的内源性因子，在骨代谢中发挥作用。