Immunopathologic studies of oral lichen planus

口腔扁平苔藓（OLP）是最常见的口腔黏膜疾病之一。临床上，OLP经常表现为多发白色花边病变，最常见于颊黏膜和舌。组织学上，OLP的特征是直接上皮亚区致密的带状淋巴细胞浸润，伴有基底上皮细胞破坏。虽然该病的发病机制被认为与细胞介导的免疫有关，但其病因尚不清楚。在本研究中，我们检查了OLP的免疫组织化学调查和人类白细胞抗原（HLA）分型。免疫组化研究表明，OLP淋巴细胞浸润主要由T细胞组成。观察到这些T细胞靠近受损的基底膜。与正常上皮组织相比，糜烂性OLP组织T淋巴细胞数量显著增加（p< 0.05）。侵蚀性OLP上皮中表达HLAⅱ类（HLA- dr、DQ、DP）的细胞数量明显高于正常（p< 0.05）。然而，mb -l阳性细胞无显著差异。因此，hla - ii阳性细胞可能激活浸润性T细胞并造成上皮损伤。HLA是细胞表面受体，是所有免疫反应中细胞间相互作用的基础。在许多情况下，发现疾病易感性与一种或多种特定HLA的反应密切相关。因此，我们测定了日本OLP患者淋巴细胞上HLA I类和II类抗原的频率。研究组17例OLP患者，对照组17例。HLA-A2、All、B35、Cwl和Cw7的表达频率在OLP中可能升高。我们还研究了HLA-DRB1、dqb1和dbb1等位基因。在一组OLP患者中发现DRB1*0405、DQB1*0301和DQB1*0401的频率略有增加。然而，在OLP患者和对照组之间，HLA的频率没有显著差异。我们将在一些OLP患者和对照组中研究HLA分型。少

Oral lichen planus (OLP) is one of the most common oral mucosal diseases. Clinically, OLP frequently presents as multiple white lacy lesions that occur most commonly on the buccal mucosa and tongue. Histologically, OLP is characterized by a dense band-like lymphocytic infiltrate in the immediate sub-epithelial region with basal epithelial cell destruction. Etiologies for OLP remain unknown, although the pathogenesis of the disease is considered to involve cell-mediated immunity. In this studies, we have examined immunohistochemical investigation and human leukocyte antigens (HLA) typing in OLP.Immunohistochernical study indicated that the OLP lymphocyte infiltrate comprises principally T cells. These T cells were observed to be close to the damaged basement membrane. Numbers of T lymphocytes were significantly increased in erosive OLP tissue compared with normal epithelium (p<O.05). Numbers of cells expressing HLA class II (HLA-DR, DQ, DP) were also increased in erosive OLP epithelium … More compared with normal (p<O.O5). However, MIB-l-positive cells did not differ significantly. Thus, HLA-II-positive cells may be activating infiltrating T cells and causing damaging epithelium.HLA are cell-surface receptors that are fundamental to cell-to-cell interaction in all immunologic responses. In many instances, close associations have been found between disease susceptibility and the response of one or more specific HLA.Therefore, we determined the frequency of HLA class I and class II antigens on lymphocytes in Japanese patients with OLP.The study group consisted 17 OLP patients and 17 control. The frequency of HLA-A2, All, B35, Cwl and Cw7 may be increased in OLP.We also studied HLA-DRB1, DQB 1 and DPB 1 alleles. Slight increase in the frequency of DRB1*0405, DQB1*0301 and DQB1*0401 found in a group of OLP patients. However, there were no significant differences in the frequency of HLA between OLP patients and control. We will be studding HLA typing in a number of OLP patients and control. Less