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Study of the low molecular weight GTP binding proteins in amylase exocytosis of parotid acini in vitro.

低分子量 GTP 结合蛋白在腮腺腺泡淀粉酶胞吐作用中的体外研究。

基本信息

批准号：
06672016
负责人：
OKUMURA Kazuhiko
金额：
$ 1.22万
依托单位：
Health Sciences University of Hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The trimeric GTP binding protein plays it as information and/or transformation factor on the plasma membranes. But existence of low molecular weight GTP binding protein which expressed in cytosol fraction. Futhermore, it is inserted in the secretory granule membranes in secretory cell and as member of constitution. So, low molecular weight GTP binding protein in parotid acinar cell exists in secretory granule membranes, and is which associated with amylase secretion. We examined whether playd it. Secretory granules of rat parotid glands were isolated by percoll-gradient centrifuges as described by Hopfer et al. We report here that at least 21ow molecular GTP-binding proteins (21 kD-25 kD) are associated with the plasma membrane and secretory granule membranes from rat parotid acini by [32 [-GTP overlay assay.Results1. The secretory granules isolated by rat parotid tissues. Western blotting analysis of the rho proteins. The rho protein A and B localized the plasma menbranes and secretory granule membranes as molecular weight at 21 kD.2. Inactivation of rho p21 of parotid acini by ADP-rybosiltransferase C3 resulted in the inhibited release of amylase, suggesting that amylase secretion was mediated by rho p21.

三聚体GTP结合蛋白作为质膜上的信息和/或转化因子发挥作用。但存在小分子量的GTP结合蛋白，表达于胞浆组分中。在分泌细胞中，它被插入分泌颗粒膜中，并作为组成成员。因此，腮腺腺泡细胞中的低分子量GTP结合蛋白存在于分泌颗粒膜上，与淀粉酶的分泌有关。用Percoll梯度离心法分离大鼠腮腺腺泡分泌颗粒，用[32]-GTP覆盖法检测到至少21个低分子量的GTP结合蛋白（21 kD-25 kD）与腺泡质膜和分泌颗粒膜结合。大鼠腮腺组织分离的分泌颗粒。rho蛋白的蛋白质印迹分析。rho蛋白A和B定位于质膜和分泌颗粒膜，分子量为21 kD。2。ADP-核糖转移酶C3对腮腺腺泡rho p21的失活导致淀粉酶释放的抑制，表明淀粉酶分泌是由rho p21介导的。