Studies on Mechanism of Offensive Odor Evolution in Porcine Small Intestine and Its Suppression by Natural Materials

(1)猪小肠(PSI)的厌氧储存不能抑制恶臭的形成，而一些化学物质，包括那些被列为合法食品添加剂的化学物质，可以延缓恶臭的形成和细菌的生长。甲硫醇(MM)是恶臭的主要挥发物，其含量与厌氧菌的细菌数量显著相关。此外，接种厌氧菌会导致产生相同的典型恶臭。这些结果表明，PSI在储存过程中恶臭的产生主要是由于厌氧细菌的生长所致。对储藏PSI中产生甲硫醇的厌氧菌进行了分离和鉴定，其中奇异变形杆菌的分离数和MM产生量均最高。(2)使用杜库达米和炼油厂成品糖蜜与某些化学物质进行的实验证实，这些物质的除臭和抗菌作用导致了对恶臭的抑制。我们从土田美中分离出一种除臭剂化合物。在不久的将来，我们将确定它的结构，并阐明它的除臭作用机制。(3)对猪大肠、牛小肠和大肠在储藏过程中挥发出的挥发物进行了分析和鉴定，挥发物的主要成分与PSI相似，微生物区系也类似于PSI。这些结果表明，在PSI的情况下获得的观察结果和发展的实验方法可以用于研究这些器官中恶臭的演变及其抑制。

(1) Anaerobic storage of porcine small intestine (PSI) was unable to inhibit offensive odor development, while some chemicals, including those listed as legal food-additives, retarded the evolution of offensive odor and bacterial growth. Amount of methyl mercaptan (MM), the main volatile responsible for the offensive odor, was found to correlate significantly with the bacterial counts of anaerobes. Furthermore, inoculation of anaerobes leaded to develop the same and typical offensive odor. These results indicate that the evolution of offensive odor during storage of PSI is mostly attributable to the growth of anaerobic bacteria. Methyl mercaptan-producing anaerobes in PSI under storage were isolated and identified, and Proteus mirabilis was the greatest both in the number of isolation and in the amounts of MM production. (2) The experiments using dokudami and refinery final molasses with some chemicals confirmed that the deodorant and antibacterial actions of those materials resulted in suppression of the offensive odor. We isolated one component of deodorant compounds from dokudami. In near future, we will determine its structure and clarify the mechanism of deodorant action of dokudami. (3) Volatiles evolved from porcine large intestine, bovine small or large intestine during storage were analyzed and identified in much the same way as those from PSI.The major components in volatiles were similar to the case of PSI,and so were microbial floras. These results suggest that the observations obtained and experimental methods developed in the case of PSI can be applied to studies on offensive odor evolution and its suppression in those organs.