Observation of Biological Specimens by a Zone-Plate X-Ray Microscope using a Laser Plasma Source

使用激光等离子体源通过波带板 X 射线显微镜观察生物样本

• 批准号: 06680652
• 负责人: ARITOME Hiroaki
• 金额: $ 1.47万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680652/
• 关键词: X-ray; Microscope; X-ray optics

Various specimens are observed by a zone-plate x-ray microscope using x-rays (wavelength 3.37 nm) from a cabon plasma generated by a moving slab glass laser (5 J,3 Hz) and a backside illuminated CCD.In the case of a metal mesh, good contrast was obtained by 5 x-ray pulses for the magnification of 100. For the sample of a zone plate 100 pulses were necessary for the magnification of 100. The resolution of 0.5 um was obtained.As a biological spcecimen, dryed Hela cells were observed. By 20 x-ray pulses, good contrast was obtained for the magnification of 100. Microvillus of about 0.5 um width could be observed.By improving x-ray optics, the improvement of resolution can be expected.

利用带板x射线显微镜，利用移动平板玻璃激光器（5j, 3hz）和背面照明CCD产生的碳等离子体所产生的x射线（波长3.37 nm）对不同样品进行观察。在金属网的情况下，通过5个x射线脉冲获得了良好的对比度，放大倍数为100。对于带片样品，要放大100倍，需要100个脉冲。得到了0.5 um的分辨率。作为生物标本，观察干燥的Hela细胞。通过20个x射线脉冲，获得了良好的对比度，放大倍数为100。微绒毛直径约0.5 um。通过改进x射线光学，可以预期分辨率的提高。

项目成果

A.Ito et al.: "Measorement of soft X-ray absorption spectra and elemental alalysis in local regions of mamimalian cells using an electronic zooingitobe" J.Microscopy. 181. 54-60 (1996)

A.Ito 等人：“使用电子动物显微镜测量哺乳动物细胞局部区域的软 X 射线吸收光谱和元素分析”J.Microscopy。

A. Ito, et al.: "Measurement of soft x-ray absorption spectra and elemental analysis in local regions of mammalian cells using an electronic zooming tobe" J. Microscopy. 181. 54-60 (1996)

A. Ito 等人：“使用电子变焦镜测量哺乳动物细胞局部区域的软 X 射线吸收光谱和元素分析”J. Microscopy。

Guan-ming Zeng: "Soft x-ray spectra of highly ionized elements with atomic numbers ranging from 57 to 82 produced by compact lasers" J. Appl. Phys.75. 1923-1930 (1994)

曾冠明：“紧凑型激光器产生的原子序数为 57 至 82 的高度电离元素的软 X 射线光谱”J. Appl。

Hiroyuki Daido, et al.: "Subnanosecond in-line soft x-ray holography using germanium laser in the 20nm wavelength region" J. X-Ray Science and Technology. 5. 105-120 (1995)

Hiroyuki Daido 等人：“在 20 nm 波长范围内使用锗激光器进行亚纳秒在线软 X 射线全息术”J. X 射线科学与技术。

Kunio Shinohara: "X-ray holographic microscopy of biological spcimens" J.Synchrotron Radiation. 3. 35-40 (1996)

Kunio Shinohara：“生物标本的 X 射线全息显微镜”J.Synchrotron Radiation。