High Resolution Confocal light microscope with incubation chamber (Teilfinanzierung(

带孵化室的高分辨率共焦光学显微镜（部分资助（

• 批准号: 538776603
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/538776603?language=en
• 关键词: Resolution; Confocal; light; microscope; incubation

The goal is to gain insights at the subcellular level into life cycles, host interactions and dynamic interactions in infection biology of highly pathogenic viruses/bacteria that need to be kept and handled under special security measurements in the BSL3/S3 biosafety laboratory. This creates a unique scientific environment to then establish innovative intervention/vaccination strategies, new industrial contacts and world-wide visibility. To do so, a solid imaging infrastructure in the BSL3/S3 environment through a high-end confocal microscope with superresolution detector is indispensable. Of note, only few research institutions with similarly equipped S3/BSL3 infrastructures are operational to date in Germany providing Freiburg a competitive position to attract collaborations and post docs. To keep the demands high in modern infection research, we need to implement a superresolution light microscope with a highly stable long-time incubation chamber for imaging of complex cellular/viral processes into our BSL3/S3 laboratory. Confocals can take images in which only the focal plane of a sample is visible resulting in an improved signal-to-noise ratio and increase in effective resolution. This is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. The super-resolution detector enables us to take images of specimens beyond the diffraction limit with an equipped climate control chamber to perform live-cell imaging/timelapse measurements. The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as bacterial BSL3 pathogens (M. tuberculosis) at the designated location of the device, but with a cross-regional relevance, offering a unique environment to pursue flagship projects and elaborate on previously unsolved questions. The device must be applied to close spatio-temporal gaps in the viral/bacterial life cycle, and to specifically use optical super-resolution to analyze highly dynamic subcellular mechanisms. Combined with a stable live-cell incubation chamber, each sample can be recorded from minutes to days, capturing rare or very short-lived events that we might otherwise miss and surely missed up to know.

目标是在亚细胞水平上深入了解高致病性病毒/细菌的生命周期、宿主相互作用和感染生物学中的动态相互作用，这些病毒/细菌需要在BSL 3/S3生物安全实验室的特殊安全措施下保存和处理。这创造了一个独特的科学环境，然后建立创新的干预/疫苗接种战略，新的工业联系和全球知名度。为此，BSL 3/S3环境中通过高端共焦显微镜和超分辨率探测器的坚实成像基础设施是必不可少的。值得注意的是，迄今为止，德国只有少数几个具有类似S3/BSL 3基础设施的研究机构在运营，这为弗赖堡提供了吸引合作和博士后的竞争优势。为了保持现代感染研究的高要求，我们需要在我们的BSL 3/S3实验室中实现具有高度稳定的长时间孵育室的超分辨率光学显微镜，用于复杂细胞/病毒过程的成像。共焦镜可以拍摄只有样品的焦平面可见的图像，从而提高信噪比并增加有效分辨率。这在广泛的实验中具有潜在的意义，例如涉及厚标本、细胞内研究和共定位的实验，以及在样品的三维结构很重要的研究中。超分辨率探测器使我们能够通过配备的气候控制室拍摄超过衍射极限的样品图像，以进行活细胞成像/时移测量。在BSL 3/S3区域（SARS-CoV、流感病毒、博尔纳病毒、猴痘病毒）建立了一个用户友好的、最先进的显微镜设施，为执行具有挑战性的分析创造了战略性的附加值，然后也对其他BSL-2病原体（腺病毒、巨细胞病毒、疱疹病毒、B肝炎病毒和M.溃疡）以及细菌BSL 3病原体（M.结核病），但具有跨区域的相关性，提供了一个独特的环境，以追求旗舰项目和阐述以前未解决的问题。该设备必须应用于关闭病毒/细菌生命周期中的时空间隙，并专门使用光学超分辨率来分析高度动态的亚细胞机制。结合稳定的活细胞孵育室，每个样本都可以从几分钟到几天进行记录，捕捉我们可能会错过的罕见或非常短暂的事件。