High Resolution Confocal light microscope with incubation chamber (Teilfinanzierung(

带孵化室的高分辨率共焦光学显微镜（部分资助（

• 批准号: 538776603
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/538776603?language=en
• 关键词: Resolution; Confocal; light; microscope; incubation

The goal is to gain insights at the subcellular level into life cycles, host interactions and dynamic interactions in infection biology of highly pathogenic viruses/bacteria that need to be kept and handled under special security measurements in the BSL3/S3 biosafety laboratory. This creates a unique scientific environment to then establish innovative intervention/vaccination strategies, new industrial contacts and world-wide visibility. To do so, a solid imaging infrastructure in the BSL3/S3 environment through a high-end confocal microscope with superresolution detector is indispensable. Of note, only few research institutions with similarly equipped S3/BSL3 infrastructures are operational to date in Germany providing Freiburg a competitive position to attract collaborations and post docs. To keep the demands high in modern infection research, we need to implement a superresolution light microscope with a highly stable long-time incubation chamber for imaging of complex cellular/viral processes into our BSL3/S3 laboratory. Confocals can take images in which only the focal plane of a sample is visible resulting in an improved signal-to-noise ratio and increase in effective resolution. This is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. The super-resolution detector enables us to take images of specimens beyond the diffraction limit with an equipped climate control chamber to perform live-cell imaging/timelapse measurements. The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as bacterial BSL3 pathogens (M. tuberculosis) at the designated location of the device, but with a cross-regional relevance, offering a unique environment to pursue flagship projects and elaborate on previously unsolved questions. The device must be applied to close spatio-temporal gaps in the viral/bacterial life cycle, and to specifically use optical super-resolution to analyze highly dynamic subcellular mechanisms. Combined with a stable live-cell incubation chamber, each sample can be recorded from minutes to days, capturing rare or very short-lived events that we might otherwise miss and surely missed up to know.

目标是在亚细胞水平上深入了解高致病性病毒/细菌的生命周期、宿主相互作用和感染生物学中的动态相互作用，这些病毒/细菌需要在 BSL3/S3 生物安全实验室的特殊安全措施下保存和处理。这创造了一个独特的科学环境，以建立创新的干预/疫苗接种策略、新的行业联系和全球知名度。为此，通过带有超分辨率探测器的高端共焦显微镜在 BSL3/S3 环境中建立可靠的成像基础设施是必不可少的。值得注意的是，迄今为止，德国只有少数拥有类似装备的 S3/BSL3 基础设施的研究机构在运营，这为弗莱堡提供了吸引合作和博士后的竞争优势。为了满足现代感染研究的高要求，我们需要在 BSL3/S3 实验室中安装具有高度稳定的长时间孵育室的超分辨率光学显微镜，用于对复杂的细胞/病毒过程进行成像。共焦可以拍摄仅样品焦平面可见的图像，从而提高信噪比并提高有效分辨率。这在广泛的实验中具有潜在的意义，例如涉及厚样本、细胞内研究和共定位的实验，以及样品的三维结构很重要的研究。超分辨率探测器使我们能够使用配备的气候控制室拍摄超出衍射极限的样本图像，以执行活细胞成像/延时测量。在 BSL3/S3 区域（SARS-CoV、流感、博尔纳病毒、猴痘）建立用户友好、最先进的显微镜设施，为执行具有挑战性的分析创造了战略附加值，然后还针对其他 BSL-2 病原体（腺病毒、巨细胞病毒、疱疹病毒、乙型肝炎病毒和溃疡分枝杆菌）以及 细菌 BSL3 病原体（结核分枝杆菌）位于设备的指定位置，但具有跨区域相关性，为开展旗舰项目和详细阐述以前未解决的问题提供了独特的环境。该设备必须用于缩小病毒/细菌生命周期中的时空间隙，并专门使用光学超分辨率来分析高度动态的亚细胞机制。与稳定的活细胞培养室相结合，每个样本都可以记录几分钟到几天的时间，捕获罕见或非常短暂的事件，否则我们可能会错过并且肯定会错过了解这些事件。