High Resolution Confocal light microscope with incubation chamber (Teilfinanzierung(

带孵化室的高分辨率共焦光学显微镜（部分资助（

• 批准号: 538776603
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/538776603?language=en
• 关键词: Resolution; Confocal; light; microscope; incubation

The goal is to gain insights at the subcellular level into life cycles, host interactions and dynamic interactions in infection biology of highly pathogenic viruses/bacteria that need to be kept and handled under special security measurements in the BSL3/S3 biosafety laboratory. This creates a unique scientific environment to then establish innovative intervention/vaccination strategies, new industrial contacts and world-wide visibility. To do so, a solid imaging infrastructure in the BSL3/S3 environment through a high-end confocal microscope with superresolution detector is indispensable. Of note, only few research institutions with similarly equipped S3/BSL3 infrastructures are operational to date in Germany providing Freiburg a competitive position to attract collaborations and post docs. To keep the demands high in modern infection research, we need to implement a superresolution light microscope with a highly stable long-time incubation chamber for imaging of complex cellular/viral processes into our BSL3/S3 laboratory. Confocals can take images in which only the focal plane of a sample is visible resulting in an improved signal-to-noise ratio and increase in effective resolution. This is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. The super-resolution detector enables us to take images of specimens beyond the diffraction limit with an equipped climate control chamber to perform live-cell imaging/timelapse measurements. The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as bacterial BSL3 pathogens (M. tuberculosis) at the designated location of the device, but with a cross-regional relevance, offering a unique environment to pursue flagship projects and elaborate on previously unsolved questions. The device must be applied to close spatio-temporal gaps in the viral/bacterial life cycle, and to specifically use optical super-resolution to analyze highly dynamic subcellular mechanisms. Combined with a stable live-cell incubation chamber, each sample can be recorded from minutes to days, capturing rare or very short-lived events that we might otherwise miss and surely missed up to know.

其目标是在亚细胞水平上深入了解高致病性病毒/细菌的生命周期、宿主相互作用和感染生物学中的动态相互作用，这些病毒/细菌需要在BSL3/S3生物安全实验室的特殊安全措施下保存和处理。这创造了一个独特的科学环境，然后建立创新的干预/疫苗接种战略、新的行业联系和世界范围的知名度。要做到这一点，在BSL3/S3环境中通过配备超分辨率探测器的高端共焦显微镜建立坚实的成像基础设施是必不可少的。值得注意的是，到目前为止，德国只有少数几家研究机构拥有类似装备的S3/BSL3基础设施，这为弗莱堡提供了吸引合作和博士后的竞争地位。为了保持现代感染研究的高要求，我们需要在我们的BSL3/S3实验室中安装一台具有高度稳定的长期孵化室的超分辨率光学显微镜，用于对复杂的细胞/病毒过程进行成像。共焦可以拍摄只有样品的焦平面可见的图像，从而改善信噪比并提高有效分辨率。这在广泛的实验中具有潜在的意义，例如那些涉及厚样品、细胞内研究和共定位的实验，以及在样品的三维结构重要的研究中。超分辨率探测器使我们能够用配备的气候控制室拍摄超出衍射极限的样品的图像，以执行活细胞成像/时间测量。在BSL3/S3地区(SARS冠状病毒、流感、博尔纳病毒、猴痘)建立一个用户友好的最先进的显微镜设施，为执行具有挑战性的分析创造了战略附加值，然后也对设备的指定位置的其他BSL-2病原体(腺病毒、巨细胞病毒、疱疹病毒、乙肝病毒和溃疡分枝杆菌)以及细菌BSL3病原体(结核分支杆菌)创造了一个独特的环境，为开展旗舰项目和阐述以前尚未解决的问题提供了一个独特的环境。该装置必须用于弥合病毒/细菌生命周期中的时空差距，并特别使用光学超分辨率来分析高度动态的亚细胞机制。与稳定的活细胞孵化室相结合，每个样本可以从几分钟到几天进行记录，捕捉到我们可能会错过的罕见或非常短暂的事件，否则我们可能会错过，也肯定会错过。