High Resolution Confocal light microscope with incubation chamber (Teilfinanzierung(

带孵化室的高分辨率共焦光学显微镜（部分资助（

• 批准号: 538776603
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/538776603?language=en
• 关键词: Resolution; Confocal; light; microscope; incubation

The goal is to gain insights at the subcellular level into life cycles, host interactions and dynamic interactions in infection biology of highly pathogenic viruses/bacteria that need to be kept and handled under special security measurements in the BSL3/S3 biosafety laboratory. This creates a unique scientific environment to then establish innovative intervention/vaccination strategies, new industrial contacts and world-wide visibility. To do so, a solid imaging infrastructure in the BSL3/S3 environment through a high-end confocal microscope with superresolution detector is indispensable. Of note, only few research institutions with similarly equipped S3/BSL3 infrastructures are operational to date in Germany providing Freiburg a competitive position to attract collaborations and post docs. To keep the demands high in modern infection research, we need to implement a superresolution light microscope with a highly stable long-time incubation chamber for imaging of complex cellular/viral processes into our BSL3/S3 laboratory. Confocals can take images in which only the focal plane of a sample is visible resulting in an improved signal-to-noise ratio and increase in effective resolution. This is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. The super-resolution detector enables us to take images of specimens beyond the diffraction limit with an equipped climate control chamber to perform live-cell imaging/timelapse measurements. The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as bacterial BSL3 pathogens (M. tuberculosis) at the designated location of the device, but with a cross-regional relevance, offering a unique environment to pursue flagship projects and elaborate on previously unsolved questions. The device must be applied to close spatio-temporal gaps in the viral/bacterial life cycle, and to specifically use optical super-resolution to analyze highly dynamic subcellular mechanisms. Combined with a stable live-cell incubation chamber, each sample can be recorded from minutes to days, capturing rare or very short-lived events that we might otherwise miss and surely missed up to know.

目的是在高度致病的病毒/细菌的感染生物学中获得亚细胞水平的洞察力，宿主相互作用以及在BSL3/S3 Biosafety Laboratory中需要保存和处理的高度致病性病毒/细菌的动态相互作用。这创建了一个独特的科学环境，然后建立创新的干预/疫苗接种策略，新的工业接触和全球知名度。为此，通过高端共聚焦显微镜在BSL3/S3环境中的实体成像基础架构是必不可少的。值得注意的是，迄今为止，在德国，只有很少有具有类似配备的S3/BSL3基础设施的研究机构在德国运作，从而为弗雷堡提供了吸引合作和后文档的竞争地位。为了保持现代感染研究的高需求，我们需要用高度稳定的长期孵育室实施超分辨率光学显微镜，以将复杂的蜂窝/病毒过程成像为我们的BSL3/S3实验室。共焦性可以拍摄图像，其中仅可见样品的焦平面，从而提高了信噪比和有效分辨率的增加。这是在广泛的实验中具有潜在感兴趣的，例如涉及厚标本，细胞内研究和共定位的实验，以及样品的三维结构很重要的研究。超分辨率检测器使我们能够使用配备有气候控制室的标本图像超出衍射极限，以执行活细胞成像/时间段测量。 The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as细菌BSL3病原体（结核分枝杆菌）在该设备的指定位置，但具有跨区域相关性，提供了一个独特的环境来追求旗舰项目并详细介绍以前未解决的问题。该设备必须应用于病毒/细菌生命周期中的时空间隙，并专门使用光学超分辨率来分析高度动态的亚细胞机制。结合一个稳定的活细胞孵化室，可以从几分钟到几天记录每个样本，捕获稀有或非常短暂的事件，我们否则可能会错过，并且肯定会错过知道。