High Resolution Confocal light microscope with incubation chamber (Teilfinanzierung(

目标是在亚细胞水平上了解需要在BSL3/S3生物安全实验室中在特殊安全措施下保存和处理的高致病性病毒/细菌的生命周期、宿主相互作用和感染生物学中的动态相互作用。这创造了一个独特的科学环境，从而建立创新的干预/疫苗接种战略、新的工业联系和全球知名度。为此，在BSL3/S3环境下，通过配备超分辨率探测器的高端共聚焦显微镜建立坚实的成像基础设施是必不可少的。值得注意的是，迄今为止，在德国只有少数拥有类似S3/BSL3基础设施的研究机构在运作，这为弗赖堡提供了吸引合作和博士后的竞争地位。为了保持现代感染研究的高要求，我们需要在BSL3/S3实验室安装一种具有高稳定的长时间孵育室的超分辨率光学显微镜，用于成像复杂的细胞/病毒过程。共聚焦镜可以拍摄图像，其中只有一个样品的焦平面是可见的，从而提高了信噪比和有效分辨率的增加。这在广泛的实验中具有潜在的兴趣，例如涉及厚标本，细胞内研究和共定位的实验，以及在样品的三维结构很重要的研究中。超分辨率探测器使我们能够拍摄超过衍射极限的标本图像，配备气候控制室进行活细胞成像/延时测量。在BSL3/S3区域（sars冠状病毒、流感病毒、博尔纳病毒、猴痘病毒）建立一个用户友好的、最先进的显微镜设备，为在设备指定位置对其他bsl2病原体（腺病毒、巨细胞病毒、疱疹病毒、乙型肝炎病毒和溃疡分枝杆菌）以及细菌性BSL3病原体（结核分枝杆菌）进行具有挑战性的分析创造了战略附加值，但具有跨区域相关性。提供一个独特的环境来追求旗舰项目和阐述以前未解决的问题。该设备必须用于关闭病毒/细菌生命周期中的时空差距，并特别使用光学超分辨率来分析高动态的亚细胞机制。结合稳定的活细胞孵育室，每个样本可以记录从几分钟到几天，捕捉罕见或非常短暂的事件，否则我们可能会错过，而且肯定会错过。

The goal is to gain insights at the subcellular level into life cycles, host interactions and dynamic interactions in infection biology of highly pathogenic viruses/bacteria that need to be kept and handled under special security measurements in the BSL3/S3 biosafety laboratory. This creates a unique scientific environment to then establish innovative intervention/vaccination strategies, new industrial contacts and world-wide visibility. To do so, a solid imaging infrastructure in the BSL3/S3 environment through a high-end confocal microscope with superresolution detector is indispensable. Of note, only few research institutions with similarly equipped S3/BSL3 infrastructures are operational to date in Germany providing Freiburg a competitive position to attract collaborations and post docs. To keep the demands high in modern infection research, we need to implement a superresolution light microscope with a highly stable long-time incubation chamber for imaging of complex cellular/viral processes into our BSL3/S3 laboratory. Confocals can take images in which only the focal plane of a sample is visible resulting in an improved signal-to-noise ratio and increase in effective resolution. This is of potential interest in a wide range of experiments, for example those involving thick specimens, intracellular studies, and colocalization, as well as in studies where the three-dimensional structure of the sample is important. The super-resolution detector enables us to take images of specimens beyond the diffraction limit with an equipped climate control chamber to perform live-cell imaging/timelapse measurements. The set-up of a user-friendly, state-of-the-art microscopy facility in the BSL3/S3 area (SARS-CoV, Influenza, Borna Virus, Monkeypox) creates a strategic added value to perform challenging analyses then also with regard to other BSL-2 pathogens (Adenoviruses, Cytomegaloviruses, Herpesviruses, Hepatitis B Viruses and M. ulcerans) as well as bacterial BSL3 pathogens (M. tuberculosis) at the designated location of the device, but with a cross-regional relevance, offering a unique environment to pursue flagship projects and elaborate on previously unsolved questions. The device must be applied to close spatio-temporal gaps in the viral/bacterial life cycle, and to specifically use optical super-resolution to analyze highly dynamic subcellular mechanisms. Combined with a stable live-cell incubation chamber, each sample can be recorded from minutes to days, capturing rare or very short-lived events that we might otherwise miss and surely missed up to know.