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An analysis of the cells derived from the olfactory placode and its possibl correlation of the migrating LHRH neurons.

对源自嗅觉基板的细胞的分析及其与迁移的 LHRH 神经元的可能相关性。

基本信息

批准号：
06680793
负责人：
MURAKAMI Shizuko
金额：
$ 0.9万
依托单位：
Juntento University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The actual migration of LHRH neurons from the nasal epithelium to the forebrain was demonstrated in the chick embryo using fluorescent dye-labeling. There were a number of labeled cells which did not co-express LHRH.These results suggest that some non-LHRH neurons in the forebrain are of olfactory placodal origin. In order to identify the other cell types migrating into the forebrain together with LHRH neurons, the tissue distribution of somatostatin (SST) immunoreactivity was studied in the nasal and forebain region in the chick embryo. On embryonic day (ED) 3.5, SST immunoreactive (-ir) cells and -ir fibers appeared in the olfactory epithelium and olfactory nerve bundles. At ED6-8, one component of the SST-ir fibers was found to separate from the olfactory nerve and it entered the medial forebrain surface. During this same period, a few SST-ir cells were observed in the medial forebrain adjacent to the SST-ir fibers. SST immunoreactivity in the nasal and forebrain areas was most striking at ED5-8 but it virtually disappeared by the day of hatching. These results indicate that the expression of SST in the nasal and forebrain region is transient in the chick embryo. Since the SST-ir cells did not co-express LHRH, it appears that SST-ir cells belong to a different cell population from LHRH neurons. However, a close relationship exist between SST-ir neural elements and LHRH neurons. This may play a role in development of LHRH neurons. Additionally, the appearance of the SST-ir cells in the forebrain at ED6-8 suggest their migraiton from the nasal region.

在鸡胚中，用荧光染料标记证实了LHRH神经元从鼻上皮向前脑的实际迁移。这些结果表明，前脑内的非LHRH神经元可能起源于嗅基板。为了确定与LHRH神经元一起迁移到前脑的其他细胞类型，研究了生长抑素（SST）免疫反应阳性细胞在鸡胚鼻区和前脑区的组织分布。在胚胎（艾德）3.5天，嗅上皮和嗅神经束中出现SST免疫反应阳性细胞和SST免疫反应阳性纤维。在ED 6 -8，发现SST-1 R纤维的一个组分从嗅神经分离，并进入内侧前脑表面。在同一时期，少数SST-IR细胞观察到在内侧前脑相邻的SST-IR纤维。SST免疫反应在鼻和前脑区是最引人注目的ED 5 -8，但它几乎消失的孵化当天。这些结果表明SST在鸡胚鼻和前脑区域的表达是短暂的。由于SST-ir细胞不共表达LHRH，因此似乎SST-ir细胞属于与LHRH神经元不同的细胞群。但SST阳性神经元与LHRH神经元之间存在密切关系。这可能在LHRH神经元的发育中起作用。此外，在ED 6 -8前脑中出现SST阳性细胞，提示它们是从鼻区迁移而来。