Transient expression of somatostatin immunoreactivity in the developing olfactory-forebrain region of the chick.

生长抑素免疫反应性在小鸡发育中的嗅前脑区域的瞬时表达。

基本信息

批准号：
08680869
负责人：
MURAKAMI Shizuko
金额：
$ 1.15万
依托单位：
Juntendo University, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680869/
关键词：
somatostatin migrating cells LHRH neurons development olfactory placode immunohistochemistry chick embryo

项目摘要

SST-ir cells and fibers appear in the olfactory-forebrain axis in early chick embryos but these SST-ir elements virtually disappear by the day of hatching (Murakami and Arai, 1994). The time of appearance and distribution of the SST-ir neural elements are similar to those of LHRH neurons which have been reported to originate in the olfactory placode and migrate into the forebrain. The results of double-staining for SST and LHRH indicate that these two types of peptidergic neurons belong to two different cell populations. The placodal epithelial cells of the embryonic day 2.5-3.5 (Stages 17-23) chick embryos were labeled with a fluorescent carbocyanine dye, DiI.Four to five days after DiI application, the labeled cells were recognized in the medial forebrain and in the septo-preoptic area. Double staining for DiI and SST in the brain showed that some of SST-ir cells in the medial forebrain were labeled with DiI.These results indicate that SST-ir cells originate in the olfactory placode and migrate into the medial forebrain. Moreover, a number of LHRH neurons in the medial forebrain surface are found to be accompanied by a small subset of transient SST-ir fibers extending from the SST-ir olfactory nerve. It is possibility that these transient SST-ir fibers provide structural support for the migration of LHRH neurons from the nasal region to the septo-preoptic area. Electron microscopic or conforcal laser microscopic study is needed to elucidate whether leading processes of LHRH neurons contact with a transient SST-ir fibers.

SST-IR细胞和纤维出现在早期鸡胚胎中的嗅觉前桥轴上，但这些SST-IR元素几乎在孵化之日消失（Murakami and Arai，1994）。 SST-IR神经元素的外观和分布与LHRH神经元的外观和分布相似，这些神经元据报道起源于嗅觉斑点并迁移到前脑。 SST和LHRH的双重染色结果表明，这两种类型的肽基神经元属于两个不同的细胞群体。胚胎天第2.5-3.5天（第17-23阶段）的斑点上皮细胞用荧光的碳糖酸染料标记，DII。涂抹后五天至五天后，在内侧前脑中和隔膜中的隔离区域中识别出标记的细胞。大脑中DII和SST的双重染色表明，内侧前脑中的某些SST-IR细胞被标记为DII。这些结果表明SST-IR细胞源自嗅觉位置，并迁移到内侧前脑。此外，发现内侧前脑表面中的许多LHRH神经元伴随着一小部分瞬态SST-IR纤维，这些临床SST-IR纤维从SST-IR嗅觉神经延伸。这些瞬态SST-IR纤维可能为LHRH神经元从鼻部区域迁移到隔preopoptic区域的迁移提供结构支持。需要电子显微镜或亲量激光显微镜研究，以阐明LHRH神经元的主要过程是否与瞬时SST-IR纤维接触。