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Transient expression of somatostatin immunoreactivity in the developing olfactory-forebrain region of the chick.

生长抑素免疫反应性在小鸡发育中的嗅前脑区域的瞬时表达。

基本信息

批准号：
08680869
负责人：
MURAKAMI Shizuko
金额：
$ 1.15万
依托单位：
Juntendo University, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680869/
关键词：
somatostatin migrating cells LHRH neurons development olfactory placode immunohistochemistry chick embryo

项目摘要

SST-ir cells and fibers appear in the olfactory-forebrain axis in early chick embryos but these SST-ir elements virtually disappear by the day of hatching (Murakami and Arai, 1994). The time of appearance and distribution of the SST-ir neural elements are similar to those of LHRH neurons which have been reported to originate in the olfactory placode and migrate into the forebrain. The results of double-staining for SST and LHRH indicate that these two types of peptidergic neurons belong to two different cell populations. The placodal epithelial cells of the embryonic day 2.5-3.5 (Stages 17-23) chick embryos were labeled with a fluorescent carbocyanine dye, DiI.Four to five days after DiI application, the labeled cells were recognized in the medial forebrain and in the septo-preoptic area. Double staining for DiI and SST in the brain showed that some of SST-ir cells in the medial forebrain were labeled with DiI.These results indicate that SST-ir cells originate in the olfactory placode and migrate into the medial forebrain. Moreover, a number of LHRH neurons in the medial forebrain surface are found to be accompanied by a small subset of transient SST-ir fibers extending from the SST-ir olfactory nerve. It is possibility that these transient SST-ir fibers provide structural support for the migration of LHRH neurons from the nasal region to the septo-preoptic area. Electron microscopic or conforcal laser microscopic study is needed to elucidate whether leading processes of LHRH neurons contact with a transient SST-ir fibers.

在早期的小鸡胚胎中，SST-ir细胞和纤维出现在嗅觉-前脑轴上，但这些SST-ir元素实际上在孵化当天就消失了（Murakami和Arai， 1994）。SST-ir神经元件的出现时间和分布与LHRH神经元相似，LHRH神经元起源于嗅觉区并迁移到前脑。SST和LHRH的双染色结果表明，这两种类型的肽能神经元属于两个不同的细胞群。用荧光碳菁染料（DiI）标记胚胎期2.5 ~ 3.5天（17-23期）的鸡胎盘上皮细胞。应用DiI后4 ~ 5天，标记的细胞在内侧前脑和隔视前区被识别。脑内DiI和SST双染色显示，内侧前脑部分SST-ir细胞被DiI标记。这些结果表明SST-ir细胞起源于嗅觉区并迁移到内侧前脑。此外，在前脑内侧表面的许多LHRH神经元被发现伴随着一小部分从SST-ir嗅神经延伸出来的瞬时SST-ir纤维。这些瞬时SST-ir纤维可能为LHRH神经元从鼻区迁移到中隔-视前区提供了结构支持。需要通过电子显微镜或共焦激光显微镜研究来阐明LHRH神经元的前导过程是否与瞬时SST-ir纤维接触。