Study for the role of microcirculation for exocrine and endocrine functions of pancreas.

研究微循环对胰腺外分泌和内分泌功能的作用。

• 批准号: 06807004
• 负责人: SEIYAMA Akitoshi
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807004/
• 关键词: Pancreas; Exocrine Function; Endocrine Function; Microcirculation; Oxygen Release; Red Blood Cell Velocity; Hemoglobin Oxygenation; Hemoglobin Concentration; Endocrine Function; Microcir culation; 膵臓; 外分泌; 微小循環; 酸素放出速度; 循環パラメーター; セクレチン; アセ

The role of microcirculation for the exocrine and endocrine functions of murine pancreas was investigated with dual-spot microspectroscopy. The study was performed from 1994 to 1995.<Results in 1994>(1) Construction of apparatus and establishment for analytical method of O_2 transport parameters in single microvessels (ca. 10 um in diameter) were succeeded with anesthetized rat liver and perfused isolated liver.(2) The above technique was applied to the investigation for the role of microcirculation for the pancreatic functions : 1) O_2 transport parameters and rate of O_2 release from exocrine pancreatic microvessels were obtained for the first time, and 2) the rate of O_2 release were coupled with the exocrine function.<Results in 1995>(1) The rate of O_2 release strongly depended on a diet, but red blood cells velocity, oxygenation of hemoglobin and intravascular hemoglobin concentration did not.(2) The rate of O_2 release was significantly reduced by inhibition of glycolysis in the murine pancreas.The mechanisms for these results should be further investigated in detail.

采用双点光谱学研究微循环对小鼠胰腺外分泌和内分泌功能的作用。这项研究从1994年到1995年进行。[结果][1994](1)用麻醉大鼠肝脏和离体灌注肝成功建立了直径约10 μ m的单根微血管O_2输运参数分析方法和仪器。(2)应用上述技术研究了微循环对胰腺功能的影响：1)首次获得了胰腺外分泌微血管的O_2转运参数和O_2释放速率，2)将O_2释放速率与胰腺外分泌功能耦合。[结果](1)O_2的释放速率与饮食密切相关，而红细胞速度、血红蛋白氧合和血管内血红蛋白浓度与饮食无关。(2)抑制糖酵解可显著降低小鼠胰腺中O_2的释放速率。这些结果的机制应进一步详细研究。

项目成果

Akitoshi Seiyama: "Assessment for rate of O_2 release from single hepatic sinusoids of rats." American Journal of Physiology. 267. H944-H951 (1994)

Akitoshi Seiyama：“评估大鼠单个肝窦的 O_2 释放率。”

A.Seiyama: "Measurement of oxygen transport parameters in single microvessels of exocrene pancreas of rat." Microcirculation annual. 10. 119-120 (1994)

A.Seiyama：“大鼠外分泌胰腺单个微血管中氧传输参数的测量。”

Akitoshi Seiyama: "Assessment for rate of O_2 release from single hepatit sinusoids of rats." American Journal of Physiology. 267. H944-H951 (1994)

Akitoshi Seiyama：“评估大鼠单个肝窦的 O_2 释放率。”

A.Seiyama: "Assessment of rate O_2 release from single hepatic sinusoids of rates." American Journal of Physiology. 267. H944-H951 (1994)

A.Seiyama：“评估单个肝窦的 O_2 释放率。”

Akitoshi Seiyama: "Tissue Perfusion and Organ Function : Ischemia/Reperfusion Injury" Elsevier Science, 263 (1996)

Akitoshi Seiyama：“组织灌注和器官功能：缺血/再灌注损伤”Elsevier Science，263 (1996)