Study for the role of microcirculation for exocrine and endocrine functions of pancreas.

用双点显微光谱仪研究了微循环对小鼠胰腺外分泌和内分泌功能的影响。结果(1)用麻醉大鼠肝脏和灌流离体肝，成功地建立了单个微血管(直径约10微米)O_2转运参数的分析装置和分析方法.(2)将上述技术应用于研究微循环对胰腺功能的作用：1)首次获得了O_2转运参数和胰腺外分泌微血管释放O_2的速率，2)O_2释放率与外分泌功能有关。(1)O_2释放率强烈依赖于饮食，而红细胞速度、血红蛋白氧合和血管内血红蛋白浓度则不受影响。(2)抑制糖酵解可显著降低小鼠胰腺O_2释放率，其机制有待进一步深入研究。

The role of microcirculation for the exocrine and endocrine functions of murine pancreas was investigated with dual-spot microspectroscopy. The study was performed from 1994 to 1995.<Results in 1994>(1) Construction of apparatus and establishment for analytical method of O_2 transport parameters in single microvessels (ca. 10 um in diameter) were succeeded with anesthetized rat liver and perfused isolated liver.(2) The above technique was applied to the investigation for the role of microcirculation for the pancreatic functions : 1) O_2 transport parameters and rate of O_2 release from exocrine pancreatic microvessels were obtained for the first time, and 2) the rate of O_2 release were coupled with the exocrine function.<Results in 1995>(1) The rate of O_2 release strongly depended on a diet, but red blood cells velocity, oxygenation of hemoglobin and intravascular hemoglobin concentration did not.(2) The rate of O_2 release was significantly reduced by inhibition of glycolysis in the murine pancreas.The mechanisms for these results should be further investigated in detail.