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Mechanism of calcification of hard tissue suggested by special calcification preceding to bone induction

骨诱导前特殊钙化提示的硬组织钙化机制

基本信息

批准号：
06807142
负责人：
YAMASHITA Kikuji
金额：
$ 1.22万
依托单位：
University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807142/
关键词：
Bone Induction Demineralized Bone Matrix Gelatin Acellular Mineral Deposition Cross-linked Collagen Calcification Tooth germ Pre-dentin op op mouse

项目摘要

In the present study, we found that an appearance of spherical mineral deposits in demineralized bone matrix gelatin (BMG) preceded the true bone formation induced by bone morphogenetic protein (BMP) and named that "acellular mineral deposition" (AMD) because of their formation independent of osteogenic cells. Still more, in order to elucidate the mechanism of AMD and its relation to hard tissue formation, heat-denatured BMG,heat-denatured demineralized tooth germ (DTG), or calf skin collagen cross-linked with glutaraldehyde was implanted, and then histological changes in the implants were observed on day 1,3,5,7 and 10 after implantation. The results showed that spherical mineralized structures, which resembled globular dentine, appeared first on day 3 both in the bone matrix of heat-denatured BMG and in the dentine matrix, but not in the pre-dentine matrix, of heat-denatured DTG.In cross-linked collagen, however, a calcification which resembled secondary calcification of bone appeare … More d first on day 3. The above post implantation calcifications all occurred independently in any hard tissue normally forming cells and matrix vesicles. These results suggests that calcification in bone and dentine formation may occur physicochemically. Thus, we propose a "Matrix Transforming Theory" for hard tissue formation : The main points of this theory are as follows. (1) Hard tissue forming cells first form the immature matrix. (2) The immature matrix is changed to any of the various calcifiable matrices. (3) The calcification physicochemically occurs in the calcifiable matrix. On the op/op mouse, It was found that endochondral ossication was suppresed stronger than intramembranous ossification, from the histological and structural study. Especially, transform from chondrocyte to osteoblaste and differentiation of osteoblasts was severly inhibited. Still more, special calcification on cartilagenous matrix was found. Now, in order to elucidate these points, we ultrastructurally and histochemically study. Less

在本研究中，我们发现脱矿骨基质明胶（BMG）中的球形矿物沉积物的出现先于骨形态发生蛋白（BMP）诱导的真骨形成，并命名为“无细胞矿物沉积”（AMD），因为它们的形成独立于成骨细胞。为进一步阐明AMD的发病机制及其与硬组织形成的关系，将热变性BMG、热变性脱矿牙胚（DTG）、戊二醛交联小牛皮胶原分别植入种植体中，观察植入后1、3、5、7、10 d的组织学变化。结果表明，热变性BMG的骨基质和热变性DTG的牙本质基质中均在第3天首先出现类似于球形牙本质的球形矿化结构，而交联胶原中则出现类似于骨次生钙化的钙化，热变性DTG的前牙本质基质中未出现类似于球形牙本质的钙化。 ...更多信息 d第3天。上述植入后钙化均独立发生在任何正常形成细胞和基质囊泡的硬组织中。这些结果表明，钙化骨和牙本质的形成可能发生物理化学。因此，我们提出了硬组织形成的“基质转化理论”：该理论的要点如下。(1)硬组织形成细胞首先形成未成熟的基质。(2)未成熟基质变成各种可钙化基质中的任何一种。(3)钙化以物理化学方式发生在可钙化基质中。在OP/OP小鼠，从组织学和结构学研究发现，软骨内骨化比膜内骨化受到更强的抑制。尤其是软骨细胞向成骨细胞转化及成骨细胞分化受到严重抑制。此外，在软骨基质上还发现了特殊的钙化，为了阐明这一点，我们对其进行了超微结构和组织化学研究。少