Induction of Odontoblast from Stromal Cell in Dental Pulp and Bone Marrow

组织工程学是继器官移植之后最受关注的医学技术。开展硬组织中骨与牙齿的组织工程研究是口腔科学研究者的使命。因此，本课题研究从牙髓和骨髓基质细胞中诱导分化成骨细胞和成牙本质细胞，以实现临床骨和牙齿再生。在牙髓研究中，我们发现H-2k^b-tsA 58小鼠永生化牙髓细胞表达DSPP基因，具有碱性磷酸酶活性，并形成羟基磷灰石，在颗粒培养中更形成软骨组织。结果表明，成年小鼠牙髓中存在可分化为成牙本质细胞和成软骨细胞的多能基质细胞。由于细胞外基质的形成对细胞的分化具有重要作用，本研究以成骨细胞MC 3 T3-E1为模型，分析了成骨细胞外基质的形成机制。 关于我们 本实验结果阐明了成骨样细胞与钛形成细胞外基质的过程。此外，我们成功地开发了组织整合的生物材料结合细胞外基质的方法分离细胞和细胞外基质。目前，以骨细胞外基质为载体，在钛板上诱导骨髓基质细胞向成骨细胞分化的研究正在进行中。在本报告中，本论文共分五章，第一章生物整合材料与组织工程--第四章细胞外基质中胶原纤维的形成开始于一个球体。第五章完整的细胞外基质作为生物材料和组织工程的应用。少

The attracted medical technology next to organ transplant is tissue engineering. It is thought to be mission entrusted to the researcher in dental science that tissue engineering of bone and tooth in hard tissue is developed. Therefore, we are studying on induction of differentiation for osteoblast and odontoblast from stromal cell in dental pulp and bone marrow, in order to carry out clinical regeneration of bone and tooth. On the study of dental pulp, we found that immortal pulp cell of H-2k^b-tsA58 mouse expressed DSPP gene, had alkaline phosphatase activity and formed hydroxyapatite, still more formed cartilage tissue in pellet culture. These results indicated that multipotential stromal cell which can differentiate to odontoblast and chondroblast presented in dental pulp of adult mouse. Then, as extracellular matrix formed by cultured cell was important for cell differentiation, the mechanism of formation of bone extra cellular matrix was analyzed on osteoblastic MC3T3-E1 cell cul … More tured on bone slice and titanium.The course of extracellular matrix formation between titanium and cultured osteoblast-like cell was clarified by the present results. Moreover, we succeeded in development of tissue integrated biomaterial combined with extracellular matrix by the method of separating cell from extracellular matrix.. Now, the study on induction of osteoblast from stromal cell in bone marrow on the titan plate with bone extracellular matrix was currying out. In the present reports, these results were explained in details in following five chapters.Chapter 1 Bio-integrated materials and tissue engineering-Bio-integrated material tender to cell.Chapter 2 A dental pulp cell has multi-potential for differentiation to odontoblast and chondroblast.Chapter 3 The role and fate of MC3T3-E1 cell cultured on bone slice.Chapter 4 Collagen fiber formation in extracellular matrix starts as a spherical body.Chapter 5 Intact extracellular matrix for use as biomaterial and in tissue engineering. Less