Active factors in crab hatch water -OHSS and a proteinase, and their role on hatching-

螃蟹孵化水中的活性因子-OHSS和蛋白酶及其对孵化的作用-

• 批准号: 06839017
• 负责人: SAIGUSA Masayuki
• 金额: $ 1.34万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06839017/
• 关键词: Marine crustaceans; Hatching mechanisms; OHSS; Proteinase; Purification; Egg-envelope structure; Hatching process; Egg-envelope digesting activity; 海産甲殻類の孵化; OHSSの精製; 卵殻構造の変化; 孵化水; 活性物質

Embryos of intertidal and estuarine crabs are clustered on the ovigerous seta of the female, and ventilated 2-4 weeks by the female's abdomen. When the embryonic development is complete, hatching occurs and zoea larvae are released into water. This study indicates that the crab hatch water (i.e., the filtered medium into which zoeas were released) contains at least two kinds of active substance : i.e., OHSS (ovigerous-hair-stripping substance) and a proteolytic enzyme. Both factors were easily separated on gel filtration.OHSS was purified by the combination of three kinds of chromatography : i.e., hydrophobic and anion-exchange chromatographies, and gel filtration. Its molecular size was 12 K in Superdex 75 (Pharmacia), but 35 K in SDS-PAGE.The proteinase dissolved debris of the egg capsule, suggesting that this enzyme actually acts on it. But this activity was at very low level, compared with casein digestion. This enzyme possibly digest a thin, sticky layr enclosing embryos, and would not act on the thick, tough layr, i.e., the main component of the egg capsule. Accordingly, it would be difficult to suppose that such a small scale dissolution causes the breakage of the egg capsule.

潮间带和河口蟹的胚胎聚集在雌性的产卵刚毛上，并通过雌性的腹部通风2-4周。当胚胎发育完成时，孵化发生，幼虫被释放到水中。这项研究表明，螃蟹孵化水（即释放幼虫的过滤介质）至少含有两种活性物质：即 OHSS（产卵脱毛物质）和蛋白水解酶。两种因子都很容易通过凝胶过滤分离。OHSS 通过三种色谱的组合进行纯化：即疏水色谱和阴离子交换色谱以及凝胶过滤。在 Superdex 75 (Pharmacia) 中其分子大小为 12 K，但在 SDS-PAGE 中为 35 K。蛋白酶溶解了蛋壳的碎片，表明该酶实际上对其起作用。但与酪蛋白消化相比，这种活性处于非常低的水平。这种酶可能会消化包裹胚胎的薄而粘的蛋层，而不会作用于厚而坚韧的蛋层，即卵囊的主要成分。因此，很难假设如此小规模的溶解会导致卵囊破裂。

项目成果

M.Saigusa: "The tidal rhythm of emergence,and ・・・・・" Biological Bulletin. 188. 166-178 (1995)

M. Saigusa：“出现的潮汐节奏，以及……”《生物通报》188. 166-178 (1995)。

M.Saigusa: "Ovigerous-hair stripping substance (OHSS) in…" Biological Bulletin. 188(印刷中(6月号)). (1995)

M. Saigusa：“……”《生物通报》188（目前正在印刷（6 月号））（1995 年）。

M.Saigusa: "Bioassay and preliminary characterization of …" Biological Bulletin. 189. 175-184 (1995)

M. Saigusa：“……的生物测定和初步表征”《生物通报》189. 175-184 (1995)。

三枝 誠行: "生物リズムの研究テクニック" 海洋と生物. 17. 252-258 (1995)

Masayuki Saegusa：“生物节律的研究技术”海洋与生物学。17. 252-258 (1995)

M.Saigusa: "A substance inducing the loss of premature embryos…" Biological Bulletin. 186. 81-89 (1994)

M. Saigusa：“一种导致过早胚胎丢失的物质……”《生物通报》186. 81-89 (1994)。