Diversity of biological clocks in the tidal environment : molecular mechanism

潮汐环境中生物钟的多样性：分子机制

• 批准号: 13839011
• 负责人: SAIGUSA Masayuki
• 金额: $ 2.24万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839011/
• 关键词: Terrestrial crab; Hatching program; Hatching-program inducing substance; circulation water; Postbranchial chamber; OHSS; Acetone; Circatidal clock; 概潮汐時計; 単離と化学構造; 孵化機構; 潮汐リズム; アセトン; プロテアーゼ; 同調; ゾエア幼生; 胚脱落誘発物質(OHSS); 分泌組織; 潮汐時計

The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg-laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching.OHSS was purified, and its active form had a molecular mass of 25 kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759 by long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33-38%. The predicted protein (54.7 kDa) secreted as a zymogen may be cleaved post-translationally, separating the C-terminal from the N-terminal region.Two years before, we found fantastic phenomena about hatching of an estuarine crab. To investigate mechanisms of hatching in decapod crustaceans, we tried to induce … More hatching by use of many kinds of chemicals. Almost chemicals are ineffective, but he found that only acetone and acetonitrile solutions can cause hatching of embryos. No embryos detached from the female hatched under the control water with aeration (400-500 ml of 5‰ artificial seawater). Hatching started around 49 hrs after the treatment with acetone for 10 min, and hatching ratio attained maximum 55 hrs after. All zoeas treated with acetone swam, and their behavior was completely the same as zoeas released from the female.The ratio of hatching was largely different according to the concentration of acetone. Ninety-five percent hatched among the embryos treated with 60% acetone, and 87% hatched among those treated with 80% acetone. Thus, the solution of 60% acetone was estimated to cause maximum ratio of hatching.At the first step of this study, we thought that hatching of S. haematocheir embryos could be caused by a substance such as acetone. Hatching is certainly caused by an active factor released from the female. But many experiments suggested that the hatching inducing substance is largely different from a chemical such as acetolie. We are analyzing this substance. Less

蟹类的卵附着系统是在产卵后在母体卵毛上形成的，孵化后从卵毛上脱落。剥毛是由一种活性因子引起的，我们称之为OHSS（卵毛剥毛物质），它在孵化时由胚胎释放。从人胚胎cDNA文库中克隆的OHSS cDNA全长1759个，编码492个氨基酸，开放阅读框（ORF）。预测的蛋白质的C-末端部分由胰蛋白酶样丝氨酸蛋白酶结构域组成，与其他动物中的对应物的同源性为33- 38%。预测的蛋白质（54.7kDa）作为酶原分泌，可能在孵化后被切割，将C-末端与N-末端区域分开。为了研究十足类甲壳动物的孵化机制，我们试图诱导 ...更多信息 用多种化学物质孵化。几乎所有的化学物质都是无效的，但他发现只有丙酮和乙腈溶液可以导致胚胎孵化。对照组（400-500 ml 5‰人工海水）中，无胚胎脱落。用丙酮处理10 min后49 h左右开始孵化，55 h后孵化率达到最大值。用丙酮处理的蚤状幼体均能游动，其行为与雌蚤释放的蚤状幼体完全相同，孵化率随丙酮浓度的不同而有较大差异。用60%丙酮处理的胚胎中有95%孵化，用80%丙酮处理的胚胎中有87%孵化。因此，60%丙酮溶液的孵化率最高。红螯蟹胚胎可能是由丙酮之类的物质引起的。孵化当然是由雌性释放的一种活跃因子引起的。但许多实验表明，孵化诱导物质与诸如丙酮之类的化学物质有很大不同。我们正在分析这种物质。少

项目成果

Saigusa, M.*, Terajima, M., M.Yamamoto: "Structure, formation, mechanical properties, and disposal of the embryo attachment system of an estuarine crab, Sesarma haematocheir."The Biological Bulletin. 203. 289-306 (2002)

Saigusa, M.*、Terajima, M.、M.Yamamoto：“河口蟹 Sesarma haematocheir 胚胎附着系统的结构、形成、机械特性和处理。”《生物通报》。

Ikeda, H., Y.Hirano, M.Saigusa: "A pair of rosette glands in the embryo and zoea larva of an estuarinecrab Sesarma haematocheir, and classification of the tegumental glands in the embryos of other crabs."Journal of Morphology. 259. 55-68 (2004)

Ikeda, H., Y.Hirano, M.Saigusa：“河口蟹 Sesarma haematocheir 胚胎和幼虫中的一对莲座腺，以及其他螃蟹胚胎中被皮腺的分类。”形态学杂志。

Azuma, H., M.Saigusa, H.Fukuda: "Sediment structures in the tidal flat of the mangrove, and remnants of organisms in the subtropical sea. (In Japanese)"Geological Journal of Okayama University (In Japanese). 9. 9-18 (2003)

Azuma, H.、M.Saigusa、H.Fukuda：“红树林潮滩中的沉积物结构和亚热带海洋中的生物残留物。（日语）”冈山大学地质学杂志（日语）。

M.Saigusa, T.Okochi, S.Ikei: "The tidal rhythm of invertebrate assemblage in the subtropical estuary : nocturnal emergence, and variety of synchrony with tidal and lunar cycles"Acta Oecologica. (In press). (2003)

M.Saigusa、T.Okochi、S.Ikei：“亚热带河口无脊椎动物群落的潮汐节律：夜间出现以及与潮汐和月球周期同步的变化”《生态学报》。

三枝誠行(分担執筆): "生物学データ大辞典(「潮汐リズム」の項目)"朝倉書店(不明). (2002)

三枝雅之（合著者）：《生物数据百科全书（“潮汐节奏”部分）》朝仓书店（未知）。