High-resolution fluorescence microscope with photomanipulation

我们通过相分离研究生物凝聚体的形成，并研究与改变细胞质材料属性相关的应激反应。研究生物相分离的标准方法是荧光显微镜。由于其工作重点，工作组需要一台精确和高分辨率的显微镜，用于定量细胞生物学和活细胞制剂(哺乳动物细胞、酵母等)的生化分析。和体外样本(生物冷凝物)。这些任务需要一台具有自动舞台和大视场的显微镜来收集统计上显著数量的事件。此外，生物过程在活细胞制剂中是暂时分解的。这些都需要用一台非常快速和灵敏的相机进行跟踪。大多数实验都需要极高的精度和稳定性，特别是在轴向焦平面上。这必须通过精确的自动保持聚焦来确保，该聚焦还允许拍摄和分析受目标温度变化影响的样品。

We study the formation of biological condensates by phase separation and investigate stress responses associated with altering the material properties of the cytoplasm. The standard method for studying biological phase separations is fluorescence microscopy. Because of its focus, the working group needs a precise and high-resolution microscope for quantitative cell biology and biochemical analysis of live cell preparations (mammalian cells, yeasts, etc.) and in vitro samples (biological condensates). These tasks require a microscope with automatic stage and large field of view to collect statistically significant numbers of events. In addition, biological processes are temporally resolved in live cell preparations. These require tracking with a very fast and sensitive camera. Most of the experiments require extreme precision and stability, especially at the axial focal plane. This must be ensured by a precise automatic holding focus, which also allows the filming and analysis of samples subjected to a targeted temperature change.