Microscopy and Image Analysis Core

摘要：显微镜和图像分析核心 拟议的COBRE将在缅因州大学（U缅因州）建立一个卓越中心，重点是 细胞外信号对细胞行为的影响。本提案中的五个相互关联的项目将 阐明细胞外信号的复杂性，这将需要复杂的和动态的分析， 体内的细胞结构。此外，所有五个拟议的研究项目都是围绕 纵向设计需要在较长时间段内对实时系统进行更高吞吐量的成像。的 五个项目还将纳入新颖的，无偏见的图像分析范式，这将增加严谨性， 再现性为了满足这些需求，拟议的COBRE显微镜和图像分析核心（MIAC） 有两个具体的目标，提供培训和访问（1）最先进的，高分辨率的实时成像服务 以及（2）实验计划、图像采集和图像分析服务以及数据存储， 管理MIAC将提供培训和获得最先进的高分辨率实时成像 服务，包括最近获得的倒置徕卡共焦/光片显微镜，直立蔡司 Airyscan 2和Olympus FV-1000共聚焦。此外，通过NIH COBRE计划提供的资金将允许 UMaine将获得一台倒置的Zeiss LSM 900，配有Airyscan 2共聚焦显微镜，用于实时成像， 传染病，这将是一个国家的独特能力。MIAC将从以下方面提供全面服务： 实验计划专家数据管理和图像分析。这些服务对于 严谨性，可重复性和最大限度地提高显微镜设备对拟议研究项目的影响 和其他用户。MIAC核心将为用户提供一对一的指导， 分析和存储他们的数据。这将包括在显微镜的理论和实践教学，以及 作为显微镜故障排除的持续支持，使用Zen，ImageJ，Matlab，Imaris和Aivia进行图像分析 软件包。MIAC核心是创新的，因为它将利用现有的工程，物理和 在成像和分析方面的生物化学专业知识，以指导项目，并利用我们的显微镜的力量， 分析软件从开始到结束在缅因大学建立显微镜和图像分析中心 将填补该州生物医学基础设施的重要空白，并促进重要的新发现， 细胞行为在多种情况下由细胞外信号传导控制。

ABSTRACT: MICROSCOPY AND IMAGE ANALYSIS CORE The proposed COBRE will establish a center of excellence at the University of Maine (UMaine) focusing on the impacts of extracellular signaling on cell behavior. The five interconnected projects in this proposal will elucidate the intricacies of extracellular signaling, which will require sophisticated and dynamic analyses of cellular structure in vivo. In addition, all five of the proposed research projects are structured around longitudinal designs that require higher throughput imaging of live systems over a longer period of time. The five projects will also incorporate novel, unbiased image analysis paradigms that will increase rigor and reproducibility. To address these needs, the proposed COBRE Microscopy and Image Analysis Core (MIAC) has two specific aims to provide training and access to (1) state-of-the art, high resolution live imaging services and (2) experiment planning, image acquisition and image analysis services as well as data storage and management. MIAC will provide training and access to state-of-the-art, high resolution live imaging services, including a recently-acquired inverted Leica confocal/lightsheet microscope, an upright Zeiss Airyscan2, and an Olympus FV-1000 confocal. In addition, funding through the NIH COBRE program will allow UMaine to acquire an inverted Zeiss LSM900 with Airyscan2 confocal microscope designed for live imaging of infectious disease, which will be a unique capacity in the state. MIAC will provide wraparound services from experimental planning to expert data management and image analysis. These services are critical for rigor, reproducibility and maximizing the impact of microscopy equipment on the proposed research projects and for other users. The MIAC core will provide one-on-one mentorship of users in planning, executing, analyzing, and storing their data. This will include instruction in the theory and practice of microscopy, as well as ongoing support for microscope troubleshooting, image analysis with Zen, ImageJ, Matlab, Imaris and Aivia software packages. The MIAC core is innovative in that it will leverage existing engineering, physics and biochemistry expertise in imaging and analysis to guide projects and exploit the power of our microscopes and analysis software from start to finish. The establishment of a Microscopy and Image Analysis Core at UMaine will fulfill important gaps in biomedical infrastructure in the state and catalyze important new discoveries in how cell behavior is governed by extracellular signaling in multiple contexts.