Two-dimensional gas chromatography system, coupled to olfactometry, flame ionization detector as well as a time of flight mass spectrometer.

二维气相色谱系统，耦合嗅觉测定法、火焰电离检测器以及飞行时间质谱仪。

• 批准号: 448729161
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/448729161?language=en
• 关键词: Two; dimensional; gas; chromatography; system

For a comprehensive sample analysis within a large number of ongoing and applied projects in the fields of sensory sciences (e.g. aroma and odor characterization, metabolite profiles), food chemistry (e.g. reactive food ingredients, lipidomics) and geology (e.g. volatile components of aquatic biogeochemical processes), a powerful, undirected analysis is required. An analysis using GCxGC-ToF-MS is indispensable in order to analyse the respective sample extracts according to the state of the art and to achieve optimal separation results. This is not possible with the heart-cut systems currently available in the research group, as these only separate selected eluents via two columns, which is why the target-compound analysis represents the primary focus here. However, the sensitivity and resolution of these systems is not suitable for sample screening (non-target analysis), especially for trace components. GCxGC modulation allows the separation of complex extracts with coeluting analytes. Due to the high resolution paired with the resulting useful application of multivariate data analysis, it is possible to detect statistical differences between samples with complex compositions. The sample composition can be determined in its full complexity and unknown active components can be evaluated qualitatively as well as quantitatively. Especially, for the planned research activities in the field of "volatilomics" and "metabolomics" as an integral part of analytics in the sensory sciences, this technology coupled with a time-of-flight mass-spectrometer (ToF-MS) is a prerequisite. By implementing the GCxGC-ToF-MS technology in the FAU laboratories, this requirement for non-target analytics will be created. This is essential for successful research in food chemistry (food safety and quality, aroma and odor research), environmental sciences (geology) and occupational medicine. The proposed GCxGC-ToF-MS is an ideal and highly flexible solution for several analytical challenges faced by research at FAU and will contribute significantly to the continuous expansion of the internationally relevant scientific success.

为了在感官科学（如香气和气味表征，代谢物谱），食品化学（如反应性食品成分，脂质组学）和地质学（如水生生物地球化学过程的挥发性成分）领域的大量正在进行和应用的项目中进行全面的样品分析，需要一个强大的，无方向的分析。使用GCxGC-ToF-MS进行分析是必不可少的，以便根据目前的技术水平分析各自的样品提取物并获得最佳分离结果。这对于研究小组目前可用的心脏切割系统来说是不可能的，因为这些系统只能通过两列分离选定的洗脱液，这就是为什么目标化合物分析是这里的主要重点。然而，这些系统的灵敏度和分辨率不适合样品筛选（非目标分析），特别是痕量成分。GCxGC调制允许分离复杂的萃取物与溶出分析物。由于高分辨率与由此产生的多变量数据分析的有用应用相匹配，可以检测具有复杂成分的样品之间的统计差异。样品组成可以确定其完整的复杂性，未知的有效成分可以定性和定量评价。特别是，在“挥发物学”和“代谢组学”领域的计划研究活动中，作为感官科学分析的一个组成部分，该技术与飞行时间质谱仪（ToF-MS）相结合是先决条件。通过在FAU实验室实施GCxGC-ToF-MS技术，将创建非目标分析的需求。这对于食品化学（食品安全和质量、香气和气味研究）、环境科学（地质学）和职业医学的成功研究至关重要。提出的GCxGC-ToF-MS是FAU研究面临的几个分析挑战的理想和高度灵活的解决方案，将为国际相关科学成功的不断扩大做出重大贡献。