Two-dimensional gas chromatography system, coupled to olfactometry, flame ionization detector as well as a time of flight mass spectrometer.

为了在感官科学(例如，香气和气味表征、代谢物特征)、食品化学(例如，活性食品成分、脂类组学)和地质学(例如，水生生物地球化学过程的挥发性成分)等领域的大量正在进行和应用的项目中进行全面的样品分析，需要一种强大的非直接分析。使用GCxGC-ToF-MS进行分析是必不可少的，以便根据最新技术对各个样品提取物进行分析并实现最佳分离结果。对于研究组目前可用的心脏切割系统来说，这是不可能的，因为这些系统只能通过两个柱分离选定的洗脱液，这就是为什么目标化合物分析是这里的主要关注点。然而，这些系统的灵敏度和分辨率不适合于样品筛选(非目标分析)，特别是对痕量组分。GCxGC调制允许将复杂的提取物与洗脱分析物分离。由于高分辨率与由此产生的多变量数据分析的有用应用相结合，有可能检测复杂成分的样本之间的统计差异。样品的组成可以完全复杂地确定，未知的活性成分可以定性和定量地评估。特别是，作为感官科学分析的一个组成部分，计划开展的“挥发性组学”和“代谢组学”领域的研究活动，这项技术与飞行时间质谱仪(ToF-MS)相结合是一个先决条件。通过在FAU实验室实施GCxGC-TOF-MS技术，将产生对非目标分析的这一要求。这对于食品化学(食品安全和质量、香气和气味研究)、环境科学(地质学)和职业医学的成功研究至关重要。拟议的GCxGC-ToF-MS是解决FAU研究面临的几个分析挑战的理想和高度灵活的解决方案，并将大大有助于不断扩大国际相关的科学成就。

For a comprehensive sample analysis within a large number of ongoing and applied projects in the fields of sensory sciences (e.g. aroma and odor characterization, metabolite profiles), food chemistry (e.g. reactive food ingredients, lipidomics) and geology (e.g. volatile components of aquatic biogeochemical processes), a powerful, undirected analysis is required. An analysis using GCxGC-ToF-MS is indispensable in order to analyse the respective sample extracts according to the state of the art and to achieve optimal separation results. This is not possible with the heart-cut systems currently available in the research group, as these only separate selected eluents via two columns, which is why the target-compound analysis represents the primary focus here. However, the sensitivity and resolution of these systems is not suitable for sample screening (non-target analysis), especially for trace components. GCxGC modulation allows the separation of complex extracts with coeluting analytes. Due to the high resolution paired with the resulting useful application of multivariate data analysis, it is possible to detect statistical differences between samples with complex compositions. The sample composition can be determined in its full complexity and unknown active components can be evaluated qualitatively as well as quantitatively. Especially, for the planned research activities in the field of "volatilomics" and "metabolomics" as an integral part of analytics in the sensory sciences, this technology coupled with a time-of-flight mass-spectrometer (ToF-MS) is a prerequisite. By implementing the GCxGC-ToF-MS technology in the FAU laboratories, this requirement for non-target analytics will be created. This is essential for successful research in food chemistry (food safety and quality, aroma and odor research), environmental sciences (geology) and occupational medicine. The proposed GCxGC-ToF-MS is an ideal and highly flexible solution for several analytical challenges faced by research at FAU and will contribute significantly to the continuous expansion of the internationally relevant scientific success.