Analysis and identification of anther-specific genes and male-sterility genes

花药特异性基因和雄性不育基因的分析和鉴定

基本信息

批准号：
14206001
负责人：
NISHIO Takeshi
金额：
$ 33.53万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14206001/
关键词：
male sterility anther-specific gene cytoplasmic male sterility fertility-restorer gene nuclear-genic male sterility mutated gene Brassicaceae plants rice 葯特異発現遺伝子

项目摘要

Exploiting the information on the genome sequences of rice and Arabidopsis, genes specifically expressed in anthers and those responsible for male sterility and fertility-restoration were analyzed using rice and Brassicaceae plants to develop novel techniques for F_1 hybrid breeding, which is presently the most reliable breeding method for improving plant productivity.Three genes controlling exine production were newly identified, and isolated from Arabidopsis. Since mutants having nonfunctional alleles of these genes produce pollen grains lacking germination ability, identification of these genes enables the production of male-sterile lines having high seed productivity in Brassicaceae crops. Using a Brassica oleracea line having a fertility-restorer gene for mur-CMS (cytoplasmic male sterility), which was selected in the present study, we found orf72 to be a candidate of the mitochondrial gene responsible for mur-CMS. We also isolated the fertility-restorer gene for Boro-CMS in rice, Rf1, first in the world, and revealed that Rf1 protein is transferred to mitochondria and interacts with mitochondrial mRNA. Fertility-restorer genes for two other types of CMS in rice were mapped on rice chromosomes, and regions harboring these genes were delimited into short parts of chromosomes. Identification of these genes will be achieved soon. In the study of mutant genes responsible for nuclear-genic male sterility, we developed a reverse genetic mutant-selection technique using Brassica petiole extract and an original, simple, efficient, and cost-effective technique for SNP analysis. These techniques are expected to contribute to the development of a novel F_1 hybrid breeding method.

使用水稻和铜科植物开发F_1混合繁殖的新技术，从而分析了在花药中专门表达的水稻和拟南芥基因组序列的信息，以及负责男性不育和生育率的基因，这是目前可从鉴定基因鉴定的，这是目前最可靠的繁殖方法。由于这些基因的非功能性等位基因的突变体产生缺乏发芽能力的花粉颗粒，因此对这些基因的鉴定可以产生胸骨科作物中具有较高种子生产力的雄性菌株。使用在本研究中选择的MUR-CMS（细胞质雄性不育）的生育能力基因（细胞质雄性不育）的胸肌橄榄酸线，我们发现ORF72是负责MUR-CMS的线粒体基因的候选者。我们还分离了世界上首先是大米，RF1中的硼-CM的生育率基因，并揭示了RF1蛋白转移到线粒体上并与线粒体mRNA相互作用。在水稻染色体上映射了针对大米中另外两种CMS的生育率成分基因，并将携带这些基因的区域界定为染色体的短部分。这些基因的识别将很快实现。在负责核基因雄性不育的突变基因的研究中，我们使用胸腔叶柄提取物开发了一种反向遗传突变体选择技术，以及一种用于SNP分析的原始，简单，高效，具有成本效益的技术。这些技术有望有助于开发一种新型的F_1混合育种方法。

项目成果

期刊论文数量（113）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Efficiency of PCR-RF-SSCP marker production in Brassica oleracea using Brassica EST seaqences.

使用甘蓝 EST 序列在甘蓝中产生 PCR-RF-SSCP 标记的效率。

DOI：
发表时间：
2004
期刊：
Euphytica 14
影响因子：
0
作者：
Inoue H;Nishio T
通讯作者：
Nishio T

Single nucleotide polymorphisms in randomly selected genes among japonica rice (Oryza sativa L.) varieties identified by PCR-RF-SSCP.

DOI：
10.1093/dnares/11.4.275
发表时间：
2004
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
0
作者：
K. Shirasawa;L. Monna;S. Kishitani;T. Nishio
通讯作者：
K. Shirasawa;L. Monna;S. Kishitani;T. Nishio

Identification of S haplotyppes in Brassica by dot blot analysis of SP11 alleles.

通过 SP11 等位基因的斑点印迹分析鉴定甘蓝中的 S 单倍型。