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Genome analysis of bovine major histocompatibility complex region and development of cattle with resistance to disease

牛主要组织相容性复合体区域基因组分析及牛抗病能力的培养

基本信息

批准号：
14206035
负责人：
AIDA Yoko
金额：
$ 33.95万
依托单位：
RIKEN
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14206035/
关键词：
bovine MHC (BoLA) region BoLA-DRB3 BoLA-DQA mastitis BLV-induced lymphoma BLV epitope diseases susceptibility PCR-sequence based typing BoLA-DQA1及び-DQA2 多型性 PCR-Sequence Based Typing BoLA-DOA及び-DQB TAPトランスポーター BoLAクラスI遺伝子 BoLA-

项目摘要

Genomic organization of bovine major histocompatibility complex (BoLA) : The draft sequence of entire bovine genome has been announced by Human Genome Sequencing Center, and the information has been available in NCBI genome project database (http://www.ncbi.nlm.nih.gov/). Here, we analyzed the comparative map of human MHC region on chromosome 6 (HSP6p) and BoLA region on chromosome 23 (BTA23), using the draft sequence of BoLA and complete sequence of human MHC region. Although the existence of two class II subregions in cattle gene map was known before (cf.class IIa and IIb), and is also confirmed in our comparative gene map based on the whole genome draft sequencing, some of the newly constructed gene correspondences show differences from the previous linkage map.Relationship between BoLA class IIa region and development of mastitis and bovine leukemia virus (BLV)-induced lymphoma : BoLA class IIa region revealed to associate in various diseases and economic trait. The class IIa region contains BoLA-DRA, -DRB, -DQA and -DQB genes and shows a high degree of polymorphism. Recently, we developed new methods for sequence based typing (SBT) which allow identification of alleles of BoLA-DQA1 gene and BoLA-DRB3 gene of large numbers of animals with relative case, and showed that the DRB3^*0701-DQA1^*0101 and DRB3^*14012-DQA1^*10012 might protect against development of BLV-induced lymphoma. We now progress to identify BLV epitope which related to both alleles. Next, we performed the typing of DQA1 alleles of 188 cattle with mastitis and 95 normal Holstein cattle and calculated each allele frequency in two groups. The DQA1^*1201 were apparently low frequency in mastitis group as compared with a normal group (p=0.006663), suggesting that this allele might be positively related to resistance to mastitis. Collectively, the breeding by the marker selection using BoLA class II haplotype might probably contribute to the improvement of disease resistance traits in domestic cattle.

牛主要组织相容性复合体(BOLA)的基因组组织：人类基因组测序中心已经公布了牛全基因组的草案序列，并已在国家生物信息学研究所基因组计划数据库(http://www.ncbi.nlm.nih.gov/).)中获得在这里，我们分析了人类6号染色体上的MHC区(HSP6p)和23号染色体上的Bola区(BTA23)的比较图谱，并利用Bola区的草稿序列和人类MHC区的完整序列进行了分析。虽然牛基因图谱中存在两个II类亚区(参见IIa和IIb类)，在我们基于全基因组草图测序的比较基因图谱中也得到了证实，但一些新构建的基因对应关系与以前的连锁图谱有所不同。BOLA IIa亚区与乳房炎和牛白血病病毒(BLV)诱导的淋巴瘤的发生关系：BOLA IIa亚区与各种疾病和经济性状相关。IIa类基因包含BOLA-DRA、-DRB、-DQA和-DQB基因，表现出高度的多态。最近，我们发展了一种新的基于序列的分型方法，可以在大量有相对病例的动物中鉴定BOLA-DQA1基因和BOLA-DRB3基因的等位基因，并表明DRB3^*0701-DQA1^*0101和DRB3^*14012-DQA1^*10012可能对BLV诱导的淋巴瘤的发生具有保护作用。我们现在正在寻找与这两个等位基因相关的BLV表位。接下来，我们对188头乳房炎牛和95头正常荷斯坦牛的DQA1等位基因进行了分型，并计算了两组中每个等位基因的频率。DQA1^*1201基因频率在乳房炎组明显低于正常组(p=0.006663)，提示该等位基因可能与乳房炎抗性呈正相关。综上所述，利用博拉II类单倍型进行标记选择的育种可能有助于家牛抗病性状的改良。