Clinical application of diagnosis method of bovine major histocompatibility complex genes associated with resistanceand susceptibility to bovine leukemia virus-induced lymphomain East Asia

我们先前证明了牛白细胞抗原(BOLA)-DRB3等位基因与牛白血病病毒(BLV)诱导的B淋巴瘤的抗性或易感性相关，并分析了这些等位基因在日本和南美的分布。然而，到目前为止，还没有一个东南亚品种的BOLA-DRB3基因的特征是基于序列的聚合酶链式反应(SBT)。因此，我们对981头来自菲律宾五个岛屿的荷斯坦牛、萨希瓦尔牛、婆罗曼牛及其杂交品种的BoLAⅡ类DRB3基因外显子2进行了测序，采用了适用于菲律宾牛的改良SBT法。此外，我们还建立了一种新的BLV-Cocomo-qPCR检测方法。我们鉴定了75个已报道的等位基因和6个新的等位基因。有趣的是，在每个品种中发现的数量都达到了一定程度的高水平，这表明亚洲品种在BOLA-DRB3分布上表现出一定程度的高度多态。我们的结果还表明，携带BLV易感相关等位基因的牛感染BLV的比例显著低于日本。

We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.