Clinical application of diagnosis method of bovine major histocompatibility complex genes associated with resistanceand susceptibility to bovine leukemia virus-induced lymphomain East Asia

牛主要组织相容性复合体基因与牛白血病病毒东亚淋巴瘤耐药和易感相关的诊断方法的临床应用

• 批准号: 22405040
• 负责人: AIDA Yoko
• 金额: $ 11.56万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22405040/
• 关键词: BoLA クラスIIDRB3; PCR-Sequence based typing(SBT)法; BLV,BLV nested PCR; フィリピン; ホルスタイン; ブラーマン; Native cattle; BoLAクラスIIDRB3; PCR-SBT法; 新規・既知アリル; 東南アジア; 固有品種; 交雑種; 多型性; BoLAクラスII DRB3・DQA1; BLV nested PCR; 中国山東省

We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.

我们先前证明了牛白细胞抗原(BoLA)-DRB3等位基因与牛白血病病毒（BLV）诱导的B淋巴瘤的抗性或易感性相关，并分析了这些等位基因在日本和南美的分布。然而，到目前为止，还没有一个东南亚品种通过pcr序列分型（SBT）鉴定出BoLA-DRB3基因。因此，我们在此对981头牛的BoLA II类DRB3基因外显子2进行了测序，这些牛包括荷斯坦牛、萨希瓦尔牛、婆罗门牛、菲律宾本地牛及其杂交牛，这些牛来自菲律宾的五个岛屿，使用适用于菲律宾牛的改良SBT方法进行了校正。此外，我们用新的BLV- cocomo - qpcr检测BLV。我们鉴定了75个先前报道的等位基因和6个新的等位基因。有趣的是，在每个品种中发现的数量都在一定程度上高水平，这表明亚洲品种的BoLA-DRB3分布具有一定程度的高多态性。我们的研究结果还显示，携带易感相关等位基因的blv感染牛明显低于日本。

项目成果

Characterization of bovine MHC class II DRB3 diversity in Latin America Creole cattle breeds

拉丁美洲克里奥尔牛品种中牛 MHC II 类 DRB3 多样性的表征

• 发表时间: 2013
• 期刊: Gene
• 影响因子: 3.5
• 作者: Giovambattista G.;Takeshima S-n.;Veronica M.R.;Matsumoto Y;Angela A. Franco;Saito H.;Onuma M.;and Aida Y
• 通讯作者: and Aida Y

Identification of bovine leukocyte antigen (BoLA) class II haprotypes that are regulated bovine leukemia virus (BLV) proviral load in Japanese Black cattle

日本黑牛中受牛白血病病毒 (BLV) 前病毒载量调节的牛白细胞抗原 (BoLA) II 类单体型的鉴定

• 发表时间: 2012
• 作者: Aida Y.;Miyasaka T.;Jimba M.;Matsumoto Y.;Kobayashi N.;Matsuhashi T.;Sentsui H.,Takeshima S-n.
• 通讯作者: Sentsui H.,Takeshima S-n.

Nuclear Import process as potential antiviral target for flu and AIDS

核进口过程作为流感和艾滋病的潜在抗病毒目标

• 发表时间: 2012
• 作者: Iwamoto N;Nishiyama A;Eiguren-Fernandez A;Hinds W;Kumagai Y;Froines JR;Cho AK;Shinyashiki M;間陽子
• 通讯作者: 間陽子

HIV-1 Vprによる細胞周期G2期停止に関与する宿主因子のハイスループットスクリーニング法の確立

HIV-1 Vpr诱导细胞周期G2期阻滞相关宿主因子高通量筛选方法的建立

• 发表时间: 2012
• 作者: 村上知行;間陽子
• 通讯作者: 間陽子

Bovine MHC class II DR molecule plays A key role in bovine leukemia virus (BLV)-induced lymphoma

牛 MHC II 类 DR 分子在牛白血病病毒 (BLV) 诱导的淋巴瘤中发挥关键作用

• 发表时间: 2011
• 作者: Yoko Aida;Shin-nosuke Takeshima;Yuki Matsumoto;Taku Miyasaka;Yoshiyuki Miyazaki;Yoshihiro Tanabe;William C.Davis;Kosuke Okada
• 通讯作者: Kosuke Okada