Clinical application of diagnosis method of bovine major histocompatibility complex genes associated with resistanceand susceptibility to bovine leukemia virus-induced lymphomain East Asia

我们以前证明了牛白细胞抗原（BoLA）-DRB 3等位基因与牛白血病病毒（BLV）诱导的B淋巴瘤的抗性或易感性相关，并分析了这些等位基因在日本和南美的分布。然而，到目前为止，没有一个东南亚品种的BoLA-DRB 3基因的特征是基于PCR序列分型（SBT）。因此，我们在这里测定了981头牛的BoLA II类DRB 3基因的外显子2，包括荷斯坦牛，Sahiwal牛，婆罗门牛，菲律宾本地牛及其杂交品种，从菲律宾的五个岛屿校正，使用适合于菲律宾牛的改良SBT方法。此外，我们通过新的BLV-CoCoMo-qPCR检测BLV。我们确定了75个先前报道的等位基因和6个新的等位基因。有趣的是，在每个品种中发现的数量都是高水平的，表明亚洲品种揭示了BoLA-DRB 3分布的高水平多态性。我们的研究结果还表明，BLV感染牛与易感性相关的等位基因显着低于日本。

We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.