Discovery of new DNA polymorphic markers and those applications to forensic medicine and humah genetics.

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1. Development and application of DNA polymorphic markersWe collected 869 chromosome data on our developed marker DXS10011, and found three new mutants.Our developed DNA marker Yfm1 was localized on long arm of Y chromosome as a multi-copy polymorphic marker.New five short tandem repeats markers on Y chromosome (DYS441, DYS442, DYS443, DYS444, DYS445) were found and developed multiplex PCR method. The multiplex PCR method for these STR markers was useful for forensic materials like old samples or mixed samples, and applicable on the distinction of the race from Japanese, white American and African American.2. Development of age related markers for forensic medicineWe found new and functionally unknown gene named as M-LP (Mpv17-like protein) on mouse kidney and spleen by differential display method. The M-LP gene was expressed as age dependent manner, and possibly regulated activities and gene expressions of antioxidant enzymes from experiments of the M-LP gene expressions in COS-7 cells.We developed a new method for the estimation of mitochondrial DNA (mtDNA) amounts on mouse organs. Amounts of mtDNA in bone marrow, spleen and lung were relatively low, and relatively high in heart and skeletal muscles. Amounts of mtDNA, translational products of mtDNA and protein expressions of mtDNA showed gradual increase according to aging in heart, lung, kidney, spleen and skeletal muscles.