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Discovery of new DNA polymorphic markers and those applications to forensic medicine and humah genetics.

新 DNA 多态性标记的发现及其在法医学和人类遗传学中的应用。

基本信息

批准号：
14207022
负责人：
MATSUKI Takasumi
金额：
$ 26.71万
依托单位：
University of Fukui (2004-2005)福井医科大学 (2002-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

1. Development and application of DNA polymorphic markersWe collected 869 chromosome data on our developed marker DXS10011, and found three new mutants.Our developed DNA marker Yfm1 was localized on long arm of Y chromosome as a multi-copy polymorphic marker.New five short tandem repeats markers on Y chromosome (DYS441, DYS442, DYS443, DYS444, DYS445) were found and developed multiplex PCR method. The multiplex PCR method for these STR markers was useful for forensic materials like old samples or mixed samples, and applicable on the distinction of the race from Japanese, white American and African American.2. Development of age related markers for forensic medicineWe found new and functionally unknown gene named as M-LP (Mpv17-like protein) on mouse kidney and spleen by differential display method. The M-LP gene was expressed as age dependent manner, and possibly regulated activities and gene expressions of antioxidant enzymes from experiments of the M-LP gene expressions in COS-7 cells.We developed a new method for the estimation of mitochondrial DNA (mtDNA) amounts on mouse organs. Amounts of mtDNA in bone marrow, spleen and lung were relatively low, and relatively high in heart and skeletal muscles. Amounts of mtDNA, translational products of mtDNA and protein expressions of mtDNA showed gradual increase according to aging in heart, lung, kidney, spleen and skeletal muscles.

1.利用我们开发的DNA多态性标记DXS 10011收集了869条染色体的数据，发现了3个新的突变体，我们开发的DNA多态性标记Yfm 1定位在Y染色体长臂上，作为一个多拷贝多态性标记，我们还发现了5个新的Y染色体短串联重复序列标记建立了DYS 441、DYS 442、DYS 443、DYS 444、DYS 445的多重PCR方法。这些STR标记的多重PCR方法适用于陈旧或混合样本等法医学材料，并适用于区分日本人、美国白色人和非洲裔美国人.年龄相关法医学标记物的建立我们利用差异显示技术在小鼠肾脏和脾脏中发现了一个新的功能未知的基因，命名为M-LP（Mpv 17-like protein）。在COS-7细胞中进行的M-LP基因表达实验表明，M-LP基因的表达具有年龄依赖性，并可能调控抗氧化酶的活性和基因表达。骨髓、脾和肺中mtDNA含量相对较低，心脏和骨骼肌中mtDNA含量相对较高。心、肺、肾、脾和骨骼肌线粒体DNA的含量、翻译产物和蛋白质表达随增龄逐渐增加。