FORENSIC, BIOCHEMICAL AND GENETIC STUDY ON GENOTYPES OF BLOOD GROUPS

血型基因型的法医、生物化学和遗传学研究

• 批准号: 03670294
• 负责人: MATSUKI Takasumi
• 金额: $ 1.34万
• 依托单位: GUNMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670294/
• 关键词: BLOOD GROUP; GENOTYPE; PHENOTYPE; ABO

(1) DEVELOPMENT OF PHENOTYPE DETERMINATION METHOD FROM DNAWE DEVELOPED A NEW SIMPLE AND RELIABLE METHOD FOR THE DETERMINATION OF O-GENE FROM DNA SAMPLES.DNA WAS EXTRACTED BY SDS-PROTEINASE K-PHENOL METHOD OR BY CHELEX-100 METHOD.O-GENE WAS AMPLIFIED BY ALLELE SPECIFIC POLYMERASE CHAIN REACTION.THE AMPLIFIED DNA WAS DETECTED BY 15% PAGE AND SILVER NITRATE STAINING.DNA FROM UNRELATED TWENTY-FIVE O PHENOTYPE PERSONS HAD O-GENE PRODUCT, AND THOSE FROM TEN AB PHENOTYPE PERSONS HAD NO PRODUCT.(2) SEROLOGIC STUDY OF ABO PHENOTYPE AND FAMILY ANALYSISSIXTY-FIVE SAMPLES FROM NINETEEN FAMILIES AND UNRELATED THREE HUNDRED EIGHTY-FIVE SAMPLES WERE COLLECTED IN THESE THREE YEARS.THERE WAS NO AA OR BB GENOTYPE PERSON FROM OUR SEROLOGIC AND FAMILY STUDIES.(3) FACTOR(S) AFFECTING ANTIGEN EXPRESSION FROM GENOME SEQUENCE IN THE ABO SYSTEMWE HAVE NO RESULT ON THIS SUBJECT IN THIS PERIOD.WE NEED MORE STUDIES TO GET THE ANSWER.

(1)DNAWE建立了一种简便、可靠的从DNA样品中检测O-基因的新方法。DNA用SDS-蛋白酶K-苯酚法或CHELEX-100法提取，用等位基因特异性聚合酶链式反应扩增O-基因。扩增出的DNA用15%PAGE和硝酸银稳定检测。25例无关O表型者的DNA有O-基因产物。(2)ABO表型和家系分析的血清学研究--这三年共收集了19个家系的5份样本和无关的385份样本，我们的血清学和家系研究中没有发现AA或BB基因型人。(3)ABO系统基因组序列中影响抗原表达的因素(S)在这一时期还没有结果，我们还需要更多的研究才能得出答案。

项目成果

Furukawa K.et al.: "Example of a woman with multiple intrauterine deaths due to anti-M who delivered a live child after plasmapheresis." Exp.Clin.Immunogenet.10. 161-167 (1993)

Furukawa K.等人：“一名因抗 M 抗体导致多例宫内死亡的妇女在血浆置换后产下活儿的例子。”

Nakajima T.et al.: "Blood group A and B glycosyltransferases in non-human primate plasma." Exp.Clin.Immunogenet.10. 21-30 (1993)

Nakajima T.et al.：“非人灵长类动物血浆中的 A 型和 B 型血糖基转移酶。”

Matsuki T.et.al.: "Approaches to Genotype Determination in the ABO System." Jpn.J.Legal Med.(suppl.). 46. 200- (1992)

Matsuki T.et.al.：“ABO 系统中基因型测定的方法”。

MIYAZAKi S et al: "Monoclonal AntiーA and AntiーA,B Antibodies from a mouse Immunized with A Secretor Saliva." Expl Clin Immunogenet. 8. 16-23 (1991)

MIYAZAKi S 等人：“来自用 A 秘书唾液免疫的小鼠的单克隆抗 A 和抗 A、B 抗体。”Expl Clin 免疫基因。

MATSUKI T et al: "Allele secific amplification method for O-gene detection" Jpn J Legal Med. 48 (suppl) (in the press). (1994)

MATSUKI T 等人：“O 基因检测的等位基因特异性扩增方法”Jpn J Legal Med。