Development of functional composite membrane and its application to the materials for dental regeneration

在含钙和磷酸盐离子的电解液中，采用水热电化学方法成功地在钛板、钛棒和钛网上形成针状磷灰石。沉积的针状磷灰石呈均匀状，垂直于基体表面。沉积的磷灰石的形态和结晶度容易受到电解条件（如电解质的组成和温度、电流密度和衬底）的调节。利用电化学石英晶体微天平对磷灰石的电化学沉积过程进行了实时监测。可见，电化学磷灰石沉积可能受电极与电解质交界处离子扩散的控制，而电流负载可能降低其活化能。我们提出了一种由定向针状磷灰石和可生物降解聚合物组成的复合膜的制备方法，用于牙科、医学和医疗组织再生。复合膜的性能易于控制；例如，这种膜的降解速率可以通过共聚物的厚度来调节，共聚物的厚度可以通过浓度和下降体积来控制。结果表明，该复合膜与软组织和硬组织均具有良好的生物相容性。将BMP应用于复合膜上，制备出生物活性膜。采用水热电化学方法将针状磷灰石涂覆在钛网上，并通过小鼠MC3T3-E1成骨样细胞的行为研究其生物相容性。成骨细胞样细胞在沉积的磷灰石上附着并伸展，对细胞的发育无不利影响。通过测定细胞外基质的碱性磷酸酶活性、胶原蛋白和骨钙素，电化学磷灰石涂层钛网的数值高于未涂层钛网。结果表明，沉积在钛网上的磷灰石具有良好的生物相容性。少

Needle-like apatites were successfully formed on titanium plate, bar, and mesh by a hydrothermal-electrochemical method in an electrolyte containing calcium and phosphate ions. The deposited needle-like apatites were homogeneously and vertically oriented to the substrate surface. Morphology and crystallinity of the deposited apatite were easily regulated by the electrolytic conditions such as composition and temperature of electrolyte, current density, and substrate.The electrochemical deposition process of apatite was real-time monitored using an electrochemical quartz crystal microbalance. It seemed that the electrochemical apatite deposition may be controlled by ion diffusion at the boundary between the electrode and the electrolyte, and the electric current loading may reduce the activation energy for it.We applied to produce a novel fabrication method of a composite membrane consisting of oriented needle-like apatite and biodegradable polymer for tissue regeneration in dentistry a … More nd medicine. The properties of the composite membrane can be easily controlled ; e.g. the degradation rate of this membrane can be regulated by the thickness of the copolymer, which can be controlled by concentration and dropped volume. It was confirmed that this composite membrane has a good biocompatibility with soft tissue and hard tissue. Furthermore, BMP was applied on this composite membrane and produced the biologically active membrane.Needle-like apatites were coated on titanium mesh using hydrothermal-electrochemical method, and its biocompatibility was investigated through the behavior of mouse MC3T3-E1 osteoblast-like cells. The osteoblast-like cells attached and extended on the deposited apatite and no detrimental effects were observed on the development of the cell. After measuring alkaline phosphatase activity as well as collagen and osteocalcin of the extracellular matrix, the electrochemically apatite-coated titanium mesh indicated higher values than the titanium mesh without coating. These results suggest that the deposited apatite on titanium mesh has excellent biocompatibility. Less