Development of cultivation techniques of wild mushrooms based on compound culture system

1.阐明了松茸菌根苗木在培养瓶中添加干酵母可以改善松茸菌根苗木的不健康生长。明确了用新建立的复合培养体系制备的培养菌丝体直接接种于苗木松苗上，可以培育出首柔蘑菇。明确了松树菌根试管苗直接移栽到苗圃条件下是一项比较实用的技术，适合于Amitake(乳牛乳杆菌)、Shouro(R.ruesens)、Shimofuri-Shimeji(T.portentosum)、Honshimeji(Lyophyllum Shimeji)蘑菇的适宜培养菌株。在平皿培养条件下，在供试的等值线菌株上，成功地进行了松茸、皂角、苦马、半红口菇等属39种菌株的结实。更多的乳汁和乳汁。我们新建立了松茸、羊肚菌和黄伞的培养菌株。在松茸中，明确了培养的菌丝体与红松、小花松和云杉在体外形成了外生菌根结构。在自然大田条件下，以及商业生产的青梅苗木接种青梅担子菌并在露地盆栽条件下保持的实验条件下，Haru-shineji(Entoloma Saepium)能够与一年生的山李幼苗形成菌根，这表明这种菌根苗是可以生产的。微型加速器观测结果表明，受扰松茸菌丝菌落恢复不快，受扰区域首先被其他菌根真菌种类侵染。明确了栗子菇(Nematoloma Subateritium)在土壤中形成根状菌，对其在土壤中的菌落扩散起着重要的作用。此外，通过显微观察，明确了根状结构由外层和内层组成。较少

1. We have clarified the unhealthy growth of mycorrhizal pine seedlings with Matsutake mushroom (Tricholoma matsutake) in vitro can be improved by the addition of dried yeast in the couture bottle.2. We have clarified that Shouro mushroom (Rhizopogon rubesens) could be cultivated by direct inoculation of nursery pine seedlings with cultured mycelia that were prepared by newly established compound culture system.3. We have clarified that direct transplantation of in vitro mycorrhizal pine seedlings to the nursery condition could be a practical technique in relation suitable culture strains of Amitake (Suillus bovinus), Shouro (R.rubesens), Shimofuri-shimeji (T.portentosum), Honshimeji (Lyophyllum shimeji) mushrooms.4. We have succeeded in the fruiting of Shimofuri-shimeji, Mine-shimeji (T.sapoaceum), Kuma-shimeji (T.terreum), and Sumizome-shimeji (L.semitale) under open-pot culture condition in the tested 64 contoured strains belonging to 39 species in the genera such as Tricholoma, Sui … More llus, and Lactarius.5. We have newly established cultured strains of Matsutake, Amigasatake (Morchella esculenta) and Chaname-tsumutake (Pholiota lubrica). In Matsutake it is clarified that cultured mycelium forms ectomycorrhizal structure in vitro with Pinus koraiensis, P. parviflora, and Picea abies.6. We have clarified that Haru-shineji (Entoloma saepium) can form mycorrhizas with one-year old Prunus mune seedlings under natural field condition as well as an experimental condition where commercially produced P.mume seedlings were inoculated with basidiospres of E.saepium and maintained in the open pot culture, indicating that such mycorrhizal seedlings could be practically produced.7. It was clarified by the minirhizotron observation that disturbed mycelial colony of matsutake does not recover rapidly and the disturbed areas are firstly colonized by other mycorrhizal fungal species.8. We have clarified that Kuritake mushroom (Naematoloma sublateritium) forms rhizomrphs in soil, which could play an important role in their colony dispersal in soil. In addition, it was clarified by the microscopic observation that the rhizomorphs consist of its outer and inner layers. Less