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Development of a high sensitivity analytical system for breath and its clinical use

高灵敏度呼吸分析系统的研制及其临床应用

基本信息

批准号：
16209020
负责人：
SHIMOUCHI Akito
金额：
$ 29.7万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

In this project, we developed a high sensitivity analytical system for human breath and applied it to clinical use. Chemical compounds in breath originate mainly from volatile metabolic products in health and diseases. Among them, hydrogen and methane are believed to represent the conditions of gastro-intestinal systems in the presence of bacterial flora. On the other hands, carbon monoxide and nitric oxide are well-known bioactive chemicals to play important roles in physiological and pathophysiological conditions. Fasted 1011 subjects aged from 20 to 88 years old volunteered for the study. After tooth brushing and gargling, fasted subjects breathed purified artificial air via a mouthpiece in a sitting position for 12 min, during which exhaled air was collected via one-way valve into the Douglas bag. Minute ventilation volumes of carbon dioxide(CO2), oxygen(O2), hydrogen(H2), methane(CH4), carbon monoxide(CO), nitric oxide(NO) and nitrogen oxide(NOX) were measuredby the standardmethod … More s. All data were normalized by body surface area. First, V02, VCO2 and VH2 were significantly decreased in age-dependent manner. Contrarily, VCH4 was increased in aged subjects. VCO was significantly increased to aging especially in women, while VNO did not significantly changed. Significant increases in VCO and decrease in VNO were observed in heavy smokers. VCO was increased in the 4th weeks after the starting date of menstruation. Age, sex, smoking habits and menstruation cycle must be concerned for the evaluation of low chemical compounds in breath. In addition to the above results, we found that (1) Dietary turmeric activated bowel motility and colonic fermentation, (2) Ethanol and acetaldehyde were detected in the skin gas after ingestion of alcohol. Their peaks appeared 30 min after those in breath and venous blood, (3) Carbon monoxide and nitrogen oxide in breath and skin gas could be reversibly and acutely altered during 2-day smoking cessation and restart even in case of a long-term cigarette smoker. Less

本研究开发了一种高灵敏度的人体呼吸分析系统，并将其应用于临床。呼吸中的化合物主要来源于健康和疾病中的挥发性代谢产物。其中，氢气和甲烷被认为代表了细菌植物群存在下胃肠道系统的状况。另一方面，一氧化碳和一氧化氮是众所周知的生物活性化学物质，在生理和病理生理条件下发挥重要作用。1011名年龄在20岁至88岁之间的志愿者禁食。空腹受试者刷牙漱口后，通过口承以坐位吸入净化人工空气12 min，期间呼出的空气通过单向阀收集到道格拉斯袋中。用标准方法测定了二氧化碳（CO2）、氧气（O2）、氢气（H2）、甲烷（CH4）、一氧化碳（CO）、一氧化氮（NO）和氮氧化物（NOX）的分钟通气量 ...更多信息 S.所有数据均通过体表面积进行标准化。首先，V02、VCO2和VH2以年龄依赖性方式显著降低。VCH_4在老年人中明显增高。随着年龄的增长，VCO显著增加，尤其是在女性中，而VNO没有显著变化。在重度吸烟者中观察到VCO显著增加和VNO降低。VCO在月经开始后第4周增加。对呼吸中低浓度化合物的评价应考虑年龄、性别、吸烟习惯、月经周期等因素。除上述结果外，我们还发现（1）膳食姜黄激活了肠道运动和结肠发酵，（2）摄入酒精后，在皮肤气体中检测到乙醇和乙醛。（3）戒烟2天后，呼气和皮肤气体中的一氧化碳和一氧化氮可发生可逆的急性变化，即使长期吸烟者也可重新开始吸烟。少