Development of a respiratory circuit for high sensitive mass spectrometry and its clinical application

高灵敏质谱呼吸回路的研制及其临床应用

• 批准号: 13358017
• 负责人: SHIMOUCHI Akito
• 金额: $ 30.78万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13358017/
• 关键词: Respiratory Circuit; Mass Spectrometry; High sensitivity; Breath; Skin gas; Ambient Pressure Ionization Mass Spectrometer; 運動負荷試験; 嫌気性代謝閾値; 呼気ガス診断; 質量分析; 高純度ガス

Cardiopulmonary exercise testing is widely used in the filed of sports medicine. Among the indices for the evaluation of exercise capacity, anaerobic threshold is one of the most important parameters, which is, however, not easy to determine. On the other hands, several studies using gas-chromatography reported that exhaled air in humans contained more than 300 unknown substances with low mass numbers. To explore the chemical compounds that may be related to anaerobic metabolism during exercise, we therefore developed a clean air-circuit system that was available for atmospheric pressure ionization mass spectrometer (APIMS) with high sensitivity. We could not detect lactic acid in exhaled air. However, we observed that exercise caused increases in the ion intensities of 60 mass numbers, or more. This finding suggests that there still remains possibility to exist unknown chemicals in the exhaled air associated with the anaerobic thresholds during exercise.Chemical compounds released fro … More m the surface of the skin are not well known, possibly because appropriate sampling methods to detect the low levels of the skin gas have not been established. Atmospheric pressure ionization mass spectrometers (APIMS) can detect very low levels of gaseous compounds. The combined use of a clean gas-trap system and APIMS has the potential of detecting low levels of metabolites in the body. In the present study, we developed a new gas-trap system for the analysis of skin gas that may contain numerous kinds of volatile compounds. The developed system consists mainly of mass-flow controllers, gas cleaners and a thermo-controlled chamber in which the cleaned gases pass. Heat sensors wee attached to the surfaces of the chamber and connecting-tube, which were surrounded by a heater. The surface of the skin was completely covered with the camber. While maintaining a constant mixing ratio, the skin gas was diluted with the same carrier gas and introduced into the APIMS. The mass spectrum of the skin gas from the palm was analyzed using both negative and positive charge modes of the APIMS. In both ionization states the spectrums were well reproduced. We next compared mass spectrums of the skin gas with those of exhaled air in the same healthy subjects. There was a significant linear relationship between ion intensities of skin gas and exhaled air. In conclusion, a new type of gas-trap system was developed for biological application of an APIMS with high sensitivity. The system successfully detected numerous kinds of volatile compounds with low mass numbers released from the skin. Less

心脏运动试验在运动医学领域有着广泛的应用。在评价运动能力的指标中，无氧阈是一个重要的参数，但它的测定并不容易。另一方面，几项使用气相色谱法的研究报告说，人类呼出的空气中含有300多种低质量数的未知物质。为了探索运动过程中可能与无氧代谢有关的化合物，因此，我们开发了一种清洁的空气回路系统，可用于高灵敏度的大气压电离质谱仪（APIMS）。我们在呼出的空气中检测不到乳酸。然而，我们观察到，运动导致60质量数或更多的离子强度增加。这一发现表明，在运动过程中呼出的空气中仍可能存在与无氧阈值相关的未知化学物质。 ...更多信息 在皮肤表面中的气体浓度并不为人所知，这可能是因为还没有建立检测低水平皮肤气体的适当取样方法。大气压电离质谱仪（APIMS）可以检测到非常低水平的气态化合物。清洁气体捕集系统和APIMS的组合使用具有检测体内低水平代谢物的潜力。在本研究中，我们开发了一种新的气体捕集系统，用于分析可能含有多种挥发性化合物的皮肤气体。所开发的系统主要由质量流量控制器，气体净化器和一个温控室，其中清洁的气体通过。热传感器贴附在腔室和连接管的表面上，腔室和连接管的周围被加热器包围。皮肤的表面完全被拱覆盖。在保持恒定混合比的同时，用相同的载气稀释表皮气体并引入APIMS。使用APIMS的负电荷和正电荷模式分析来自手掌的皮肤气体的质谱。在两种电离状态下，光谱都能很好地再现。接下来，我们比较了相同健康受试者的皮肤气体和呼出气体的质谱。皮肤气体离子强度与呼出气离子强度呈显著线性关系。总之，开发了一种新型的气体捕集系统，用于高灵敏度的APIMS生物应用。该系统成功地检测了多种低质量数的挥发性化合物从皮肤释放。少

项目成果

Ukai K, Shimouchi A et al.: "Evaluation of non-physical stress based on the relationship between heart rate and physical activity."Jpn J Physiol. 54. S94 (2004)

Ukai K、Shimouchi A 等人：“基于心率和身体活动之间的关系评估非身体压力。”Jpn J Physiol。

Shimouchi A et al.: "Factors related to life satisfaction and subjective healthy feelings in respiratory patients during home oxygen therapy"American Journal of Respiratory and Critical Care Medicine. 163(5). A59 (2001)

Shimouchi A等人：“呼吸系统患者家庭氧疗期间生活满意度和主观健康感受的相关因素”美国呼吸与重症监护医学杂志。

Shimouchi A et al.: "Factors related to the feelings of burden in care-givers for respiratory"American Journal of Critical and Care Medicine. 165:8. A832 (2002)

Shimouchi A 等人：“与呼吸系统护理人员负担感相关的因素”美国重症与护理医学杂志。

Hasuda T, Shimouchi A et al.: "Improvement in exercise capacity with nitric oxideinhalation in patients with pulmonary hypertension."Circulation. 101. 2066-2070 (2000)

Hasuda T、Shimouchi A 等人：“吸入一氧化氮可改善肺动脉高压患者的运动能力。”循环。

Shirai M, Shimouchi A.et al.: "Two-week, but not 1-week, hypoxic exposure enhances nitric oxide-mediated basal tone regulation in rat resistance pulmonary arteries"Japanese Journal of Physiology. 51(3). 395-398 (2001)

Shirai M、Shimouchi A.等人：“两周而非一周的缺氧暴露增强了大鼠阻力肺动脉中一氧化氮介导的基础张力调节”《日本生理学杂志》。