Genome-wide microsatellite mapping of multi-factorial ocular diseases.

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In order to identify susceptibility genes associated with ocular diseases and hypertension, we have performed association study, using 25000 microsatellites(MS) and pooled DNA. The progresses of each disease is as follows.High myopia : 534 samples were collected. After the first, second and third screen, 156 positive markers were passed. As a result of indivisual typing, 26 positive markers were obtained. SNP typings were performing in the surrounding each candidate regionBehcet disease : 427 samples were collected. The first, second and third MS screenings and individual genotyping were finished, and 11 positive markers were obtained. SNP typing are performing in the surrounding each candidate regions.Lattice degeneration : 397 samples were collected. In the first screening, we found significant association for 2851 markers.Hypertension : 508 samples were collected. MS screenings were finished and 135 positive markers were obtained. Individual typing then were performed, and 55 markers were passed. SNP genotyping are performing now.Glaucoma : 192 samples were collected. The first screen using 138 samples was performed and 13.8% positive markers were obtained. The second screen is performing now. Simultaneously, SNP typings using GeneChip are performing.