Confocal Microscope with Patch Clamp Set-Up

带膜片钳装置的共焦显微镜

• 批准号: 450471400
• 金额: --
• 依托单位: Medizinische Hochschule Hannover (MHH)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/450471400?language=en
• 关键词: Confocal; Microscope; Patch; Clamp; Set

We are applying for an upright confocal microscope for combination with electrophysiology equipment. The planned investment is intended to create the essential structural prerequisite for the newly-appointed director of the Institute of Neurophysiology at the MHH to be able to carry out the applicant's central research projects in the Collaborative Research Centres TRR and SFB as well as in existing and planned collaborations at the MHH in the fields of endo-lysosomal cation channels, infection research, cardiovascular physiology and neurophysiology. The investment is therefore not only of essential importance for the work at the Institute of Neurophysiology but also for the development of new cooperation networks and research alliances at the MHH and beyond. The requested confocal microscope will be made available to the MHH research community through its virtual integration into the Research Core Unit for Laser Microscopy. This will substantially contribute to the visibility of the MHH as a nationally and internationally recognized imaging location.With the requested upright confocal microscope we will 1.) perform experiments on fixed cells, 2.) study endo-lysosomal trafficking in permanent cell lines and primary cells, 3.) perform Ca2+ imaging and 4.) FRET experiments on vital sinus node preparations or heart sections combined with patch clamp, 5.) investigate interactions of immune cells with different interaction partners using high resolution microscopy in vitro and in vivo in the anesthetized animal.

我们正在申请一台立式共聚焦显微镜，用于与电生理设备相结合。计划的投资旨在为MHH神经生理学研究所新任命的主任创造必要的结构先决条件，以便能够在合作研究中心TRR和SFB以及MHH在内溶酶体阳离子通道，感染研究，心血管生理学和神经生理学领域的现有和计划的合作中开展申请人的中心研究项目。因此，这项投资不仅对神经生理学研究所的工作至关重要，而且对在MHH及其他地方发展新的合作网络和研究联盟也至关重要。所要求的共聚焦显微镜将通过其虚拟集成到激光显微镜的研究核心单元提供给MHH研究社区。这将大大有助于MHH作为国家和国际公认的成像位置的可见度。在固定的细胞上进行实验，2.）研究永久细胞系和原代细胞中的内溶酶体运输，3.）进行Ca 2+成像和4.）在重要窦房结制备物或心脏切片上的FRET实验与膜片钳结合，5.）使用高分辨率显微镜在体外和麻醉动物体内研究免疫细胞与不同相互作用伴侣的相互作用。