Confocal Microscope with Patch Clamp Set-Up

我们正在申请一种立式共聚焦显微镜，用于与电生理设备相结合。计划中的投资旨在为MHH新任命的神经生理学研究所所长创造必要的结构前提，使其能够在合作研究中心TRR和SFB以及MHH在内切酶阳离子通道、感染研究、心血管生理学和神经生理学领域的现有和计划合作中开展申请人的中心研究项目。因此，这笔投资不仅对神经生理学研究所的工作至关重要，而且对于在卫生部及其他地方发展新的合作网络和研究联盟也是至关重要的。所要求的共焦显微镜将通过虚拟集成到激光显微镜研究核心单位向MHH研究界提供。这将大大提高MHH作为国家和国际公认的成像地点的可见度。使用所要求的直立式共聚焦显微镜，我们将1。在固定细胞上进行实验，2。)研究永久性细胞系和原代细胞中的内切溶酶体转运，3.执行钙离子成像和4.)重要的窦房结准备或心脏切片结合膜片钳的FRET实验，5。在麻醉动物体内和体外用高分辨率显微镜研究免疫细胞与不同相互作用伙伴的相互作用。

We are applying for an upright confocal microscope for combination with electrophysiology equipment. The planned investment is intended to create the essential structural prerequisite for the newly-appointed director of the Institute of Neurophysiology at the MHH to be able to carry out the applicant's central research projects in the Collaborative Research Centres TRR and SFB as well as in existing and planned collaborations at the MHH in the fields of endo-lysosomal cation channels, infection research, cardiovascular physiology and neurophysiology. The investment is therefore not only of essential importance for the work at the Institute of Neurophysiology but also for the development of new cooperation networks and research alliances at the MHH and beyond. The requested confocal microscope will be made available to the MHH research community through its virtual integration into the Research Core Unit for Laser Microscopy. This will substantially contribute to the visibility of the MHH as a nationally and internationally recognized imaging location.With the requested upright confocal microscope we will 1.) perform experiments on fixed cells, 2.) study endo-lysosomal trafficking in permanent cell lines and primary cells, 3.) perform Ca2+ imaging and 4.) FRET experiments on vital sinus node preparations or heart sections combined with patch clamp, 5.) investigate interactions of immune cells with different interaction partners using high resolution microscopy in vitro and in vivo in the anesthetized animal.