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Sustainable carbon cycle toward mitigation of carbon dioxide emission and regeneration of hydrocarbon resources

可持续碳循环，以减少二氧化碳排放和碳氢化合物资源再生

基本信息

批准号：
17206090
负责人：
SATO Kozo
金额：
$ 17.56万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17206090/
关键词：
global warming carbon dioxide bio-technology applied microbial engineering batch culture

项目摘要

Carbon dioxide capture and storage (CCS) is one of the feasible technological options for reducing net CO2emissions to the atmosphere. However, further studies on uncertainty parameter such as detention period and leakage CO2 are required toward the practical use. In order to establish sustainable carbon cycle, dPevelopment of carbon conversion processes capable of converting CO2 stored in subsurface by CCS into reusable sources of energy is desired. Regeneration of methane gas pool using microbial processes is thought to be one of the technical options addressing for this issue.The objectives of the study are to assess the effect of carbon dioxide on the mixed culture system, to increase methane yield by adding methane-producing bacteria that utilize metabolites of hydrogen-producer as substrates, and to accumulate knowledge in the mixed culture system with a view of future modeling. In the mixed culture of HYH-1 and M. thermolithotrophicus, methane production with carbon dioxide gas … More phase was 1.6-fold of that with nitrogen gas-phase. The result showed that more substrate becomes available for acetic acid fermentation, which is the most appropriate pathway for hydrogen production. Next, a methane-producing bacterium that assimilates acetic acid, a main metabolite of hydrogen-producing bacteria, was added to the culture in order to improve methane yield. Methane production increased to 1.5-fold by adding an acetic acid assimilating bacterium, Methanosarcina thermophila, to the two-species mixed culture.For recovering energy from carbon dioxide sequestrated in subsurface, geochemical effects of acidic and substrate- and nutrient-limiting conditions on methane production by a hydrogenotrophic methanogen Methanothermobacter thermautotrophicus were investigated under simulated in situ conditions of deep saline aquifers using two formation waters retrieved from petroleum reservoirs as medium. Volumetric production rate of methane by anaerobically growing Methanothermobacter thermautotrophicus cells in a defined medium was 2-fold lower at pH5.5 than at pH8.0 and was significantly reduced but still retained 1.5% of productivities at the low pH in combination with nutrient-limiting conditions employing two formation waters as growth media. Less

二氧化碳捕获与封存（CCS）是减少CO2净排放的可行技术方案之一。但滞留期、CO2泄漏量等不确定性参数还有待进一步研究。为了建立可持续的碳循环，需要开发能够将通过CCS储存在地下的CO2转化为可重复使用的能源的碳转化工艺。利用微生物法再生甲烷气藏是解决这一问题的技术选择之一，本研究的目的是评估二氧化碳对混合培养系统的影响，通过添加利用产氢菌代谢产物作为底物的产甲烷菌来提高甲烷产量，并为混合培养系统的建模积累知识。在HYH-1与M.嗜热石养菌，用二氧化碳气体生产甲烷 ...更多信息相是氮气相的1.6倍。结果表明，乙酸发酵可利用的底物较多，是最适宜的产氢途径。接下来，将同化乙酸（产氢细菌的主要代谢物）的产甲烷细菌添加到培养物中以提高甲烷产率。通过在两种混合培养物中添加乙酸同化细菌嗜热甲烷八叠球菌（Methanosarcina thermophila），甲烷产量增加到1.5倍。酸性、基质和养分的地球化学效应在模拟深层咸水层的原位条件下，使用两种不同的微生物对氢营养型甲烷菌产甲烷的限制条件进行了研究。从油藏中回收的地层沃茨为介质。在限定培养基中厌氧生长的热自养甲烷热杆菌细胞的甲烷体积生产率在pH5.5时比在pH8.0时低2倍，并且显著降低，但在低pH结合采用两种地层沃茨作为生长培养基的营养限制条件下仍保留1.5%的生产率。少