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Experimental Study of Osteoporosis

骨质疏松症的实验研究

基本信息

批准号：
63570693
负责人：
SATO Kozo
金额：
$ 1.54万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

[Purpose of the study] The effect of risk factors of developing osteoporosis including malabsorption, ovariectomy, low calcium diet, and diabetes was experimentally evaluated.[Materials and Methods] Malabsorption was induced by pancreatoduodenectomy in 10 adult beagles. Half of them were reconstructed by Child's procedure, and the remaining by modified one. Five dogs survived for 6 months, two of them operated by Child's procedure, and the others by modified method, were killed after giving tetracycline twice intravenously. Undecalcified cut sections of 3rd lumbar spine were evaluated histomorphometrically.Wistar-retired rats aging 7 to 8 months were sustained risk factors consisting of ovariectomy, low calcium diet, diabetes induced by streptozotocin injection. They were loaded either alone or in combination with one or two. Low calcium diet contains 6.0mg calcium/100g. Streptozotocin was given intraperitoneally at dose of 30mg/kg, and diabetes was detected by hyperglycemia more than … More 300mg/dl. Sham-operated rats were served as controls. They were sacrificed at 3 or 6 months of experiment after tetracycline labeling. Undecalcified ground sections of the proximal tibia, stained with Villanueva method, were histomorphometrically evaluated with Cosmozone 1S (Nikon).[Results and discussion] All dogs lost their weight by 0.3 to 5kg, and they all sustained malabsorption of fat and lost their tolerance to glucose. Mean value of bone volume did not show significant decrease in pancreatodudenectomized dogs, except for one operated by Child's procedure. Parameters relating to bone formation including an extent of osteoid surface and tetracycline labeling were depressed compared with controls. This impaired bone formation would eventually bring about bone loss.All rats sustained both single factor and in combination with one or two lost their bone volume significantly at 3 months of experiment. Decrease of bone volume at 6 months was more apparent, particularly in diabetes-sustaind rats. The effect of combination of risk factors on bone loss was more dominant compared with that of single factor. Bone formation rate at 3 months of experiment was depressed in diabetes rats in particular, mostly because of impaired matrix formation. Less

[研究目的]通过实验评估吸收不良、卵巢切除、低钙饮食和糖尿病等骨质疏松危险因素的影响。[材料与方法]对10只成年比格犬进行胰十二指肠切除术诱导吸收不良。其中一半是通过柴尔德手术重建的，其余的是改良手术。 5只狗存活了6个月，其中两只采用柴尔德手术，其余采用改良方法，静脉注射四环素两次后处死。对第三腰椎未脱钙切片进行组织形态计量学评价。7～8月龄Wistar退役大鼠为卵巢切除、低钙饮食、链脲佐菌素注射诱发糖尿病等持续危险因素。它们要么单独装载，要么一两个一起装载。低钙饮食含钙6.0mg/100g。腹腔注射链脲佐菌素，剂量为 30mg/kg，通过高血糖超过 300mg/dl 检测出糖尿病。假手术大鼠作为对照。在四环素标记后实验3或6个月时将它们处死。用Villanueva法染色的胫骨近端未脱钙地面切片，用Cosmozone 1S（Nikon）进行组织形态计量学评估。 [结果与讨论]所有狗的体重减轻了0.3至5kg，并且它们都持续脂肪吸收不良并失去对葡萄糖的耐受性。除了一只接受Child手术的狗外，胰十二指肠切除犬的骨体积平均值并未显示显着下降。与对照组相比，与骨形成相关的参数（包括类骨质表面的范围和四环素标记）均降低。这种受损的骨形成最终会导致骨质流失。在3个月的实验中，所有大鼠都经历了单一因素和其中一种或两种因素的组合，其骨量明显减少。 6 个月时骨量减少更为明显，尤其是患有糖尿病的大鼠。与单一因素相比，危险因素组合对骨丢失的影响更为显着。实验 3 个月后的骨形成率在糖尿病大鼠中尤其明显，主要是因为基质形成受损。较少的