Experimental Study of Osteoporosis

[研究目的]通过实验评估吸收不良、卵巢切除术、低钙饮食和糖尿病等骨质疏松症危险因素的影响。[材料与方法] 10只成年比格犬行胰管切除术，造成吸收障碍。其中一半采用Child法重建，另一半采用改良法重建。5只犬存活6个月，其中2只采用Child法，其余采用改良法，静脉注射四环素2次后处死。7 ~ 8月龄Wistar退休大鼠卵巢切除、低钙饮食、注射链脲佐菌素诱发糖尿病为持续性危险因素。它们要么单独装载，要么与一个或两个组合装载。低钙日粮含钙6.0mg/100 g。腹腔注射链脲佐菌素30 mg/kg，血糖≥ 10 mg/kg， ...更多信息 300毫克/分升。假手术组大鼠作为对照。四环素标记后3个月或6个月处死。使用Cosmozone 1 S（Nikon）对采用Villanueva方法染色的胫骨近端未脱钙研磨切片进行组织形态计量学评价。[结果与讨论]所有实验犬体重减轻0.3 ~ 5 kg，脂肪吸收不良，糖耐量下降。除Child手术组外，胰十二指肠切除组的骨体积平均值均无明显下降。与对照组相比，与骨形成相关的参数（包括类骨质表面和四环素标记的程度）被抑制。实验3个月时，无论是单因素组还是单因素+双因素组，大鼠的骨量均明显减少。6个月时骨体积下降更明显，尤其是糖尿病大鼠。多因素联合作用对骨丢失的影响比单因素作用更显著。尤其是在糖尿病大鼠中，实验3个月时的骨形成率受到抑制，主要是因为基质形成受损。少

[Purpose of the study] The effect of risk factors of developing osteoporosis including malabsorption, ovariectomy, low calcium diet, and diabetes was experimentally evaluated.[Materials and Methods] Malabsorption was induced by pancreatoduodenectomy in 10 adult beagles. Half of them were reconstructed by Child's procedure, and the remaining by modified one. Five dogs survived for 6 months, two of them operated by Child's procedure, and the others by modified method, were killed after giving tetracycline twice intravenously. Undecalcified cut sections of 3rd lumbar spine were evaluated histomorphometrically.Wistar-retired rats aging 7 to 8 months were sustained risk factors consisting of ovariectomy, low calcium diet, diabetes induced by streptozotocin injection. They were loaded either alone or in combination with one or two. Low calcium diet contains 6.0mg calcium/100g. Streptozotocin was given intraperitoneally at dose of 30mg/kg, and diabetes was detected by hyperglycemia more than … More 300mg/dl. Sham-operated rats were served as controls. They were sacrificed at 3 or 6 months of experiment after tetracycline labeling. Undecalcified ground sections of the proximal tibia, stained with Villanueva method, were histomorphometrically evaluated with Cosmozone 1S (Nikon).[Results and discussion] All dogs lost their weight by 0.3 to 5kg, and they all sustained malabsorption of fat and lost their tolerance to glucose. Mean value of bone volume did not show significant decrease in pancreatodudenectomized dogs, except for one operated by Child's procedure. Parameters relating to bone formation including an extent of osteoid surface and tetracycline labeling were depressed compared with controls. This impaired bone formation would eventually bring about bone loss.All rats sustained both single factor and in combination with one or two lost their bone volume significantly at 3 months of experiment. Decrease of bone volume at 6 months was more apparent, particularly in diabetes-sustaind rats. The effect of combination of risk factors on bone loss was more dominant compared with that of single factor. Bone formation rate at 3 months of experiment was depressed in diabetes rats in particular, mostly because of impaired matrix formation. Less