Development of New Chemistry-based Methods for Protein Modification and Engineering That can be Applied under Crude Cellular Conditions

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Chemistry-based selective labeling of an intended target protein under very complicated crude systems like cell, tissues or in vivo is an important and challenging project in chemical biology for understanding and controlling the protein functions under such really working conditions. However, it is absolutely difficult because of many obstacles. My group in this research grant successful developed three distinct and new methods to label and modify a protein of interest under crude conditions like cell lysates, live cell or in vivo by elaborately combining the sophisticated molecular recognition events with orthogonal and selective organic reactions applicable even in aqueous medium.