Structural biology of cytoskeletal control by dynamic protein complex formation.

点击翻译按钮获取中文摘要

We pursued structural studies of signaling proteins important in cell biology and enzymes that play a role in maintenance of genome stability. Using X-ray crystallography and NMR, we revealed three-dimensional structures of the PH-C1-PH domain of Rho-kinase, the radixin FERM domain-CD44 complex, the PHCCEx domain of RacGEF Tiam1,the CAP-Gly domain of CLIP-170 complexed with the acidic tail of α-tubulin, the gibberellin receptor bound to the effector protein DELLA, the trimeric G protein Gq-YM254890 complex and the WRN-DNA complex and elucidated their structure-function relationships at atomic resolution.