Structural Studies of Molecular Recognition by Proteins : X-ray Analyses of Complex Crystals

蛋白质分子识别的结构研究：复杂晶体的 X 射线分析

• 批准号: 09308025
• 负责人: HAKOSHIMA Toshio
• 金额: $ 21.31万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09308025/
• 关键词: transcription; signal transduction; G protein; protein; DNA; molecular complex; X-ray; structural biology; 転写因子; G蛋白質; 二成分系

Crystal structures of three transcription factor/DNA complexes, PHO4/DNA, IRF-2/DNA and Pap1/DNA, were determined. Based on these structures, molecular mechanisms of base recognition by these proteins were elucidated. In the mammalian signaling pathways, crystal structures of the small G protein RhoA in the GTPγS-MgィイD12+ィエD1 bound from and its complex with the effector protein PKN were determined. The structures reveals Rho-specific switching mechanisms and its recognition of the effector protein. Moreover, crystal structures of the thioredoxin-peroxidase HBP23 and a hippocampal serine protease, neuropsin, were determined. In the bacterial His-Asp phosphorelay signaling pathways, crystal structures of the HPt domain of a sensor kinase ArcB and its protein phosphatase SixA were determined.

确定了三种转录因子/DNA复合物的晶体结构，即PHO4/DNA，IRF-2/DNA和PAP1/DNA。基于这些结构，阐明了这些蛋白质碱识别的分子机制。在哺乳动物信号通路中，确定了GTPγS-MGYI D12+IYE D1中小G蛋白RhoA的晶体结构，并确定其与效应蛋白PKN的复合物。这些结构揭示了Rho特异性开关机制及其对效应蛋白的识别。此外，确定了硫氧还蛋白 - 过氧酶HBP23和海马丝氨酸蛋白神经蛋白的晶体结构。在细菌HIS-ASP磷化信号通路中，确定了传感器激酶ARCB的HPT结构域及其蛋白磷酸酶SIXA的晶体结构。

项目成果

Y.Fujii: "Crystallographic characterization of Pap1-DNA..." Acta Cryst.D54. 1014-1016 (1998)

Y.Fujii：“Pap1-DNA 的晶体学表征……”Acta Cryst.D54。

Kishi,T., Kato,M., Shimizu,T., Kato,K., Matsumoto,K., Yoshida,S., Shiosaka,S. and Hakoshima,T.: "Crystallization and Preliminary X-ray Analysis of a serine protease expressed in the limbic system of brain, Neuropsin"J. Struct. Biol.. 118(3). 248-251 (1997

岸T.、加藤M.、清水T.、加藤K.、松本K.、吉田S.、盐坂S.

M.Kato: "Crystallization and Preliminary X-ray Analysis..." Acta Cryst.D^-. D54. 140-142 (1998)

M.Kato：“结晶和初步 X 射线分析……”Acta Cryst.D^-。

T.Shimizu: "Crystal Structure of PHO4 bHLH domain-DNA complex" EMBO Journal. 16・15. 4689-4697 (1997)

T.Shimizu：“PHO4 bHLH结构域-DNA复合物的晶体结构”EMBO杂志16・15（1997）。

Y.Sugimoto: "Dechloroacutumine, a possible biosynthetic..." Phytochemistry. 49. 1293-1297 (1998)

Y.Sugimoto：“脱氯尖刺碱，一种可能的生物合成物……”植物化学。